Improved breeding of fugu following whole genome Sequencing

2012 Fiscal Year Final Research Report

• Project/Area Number: 20228003
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: The University of Tokyo
• Principal Investigator: SUZUKI Yuzuru 東京大学, 大学院・農学生命科学研究科, 教授 (40107412)
• Co-Investigator(Renkei-kenkyūsha): KIKUCHI Kiyoshi 東京大学, 大学院・農学生命科学研究科, 助教 (20292790) ; SUETAKE Hiroaki 福井県立大学, 海洋生物資源学部, 准教授 (00334326)
• Project Period (FY): 2008 – 2012
• Keywords: 遺伝育種 / トラフグゲノム / 耐病性 / 体サイズ / 行動 / 棘 / 性決定

Research Abstract

As the first commercially valuable fish species, tora-fugu(Takifugu rubripes) genome was sequenced. We tried to utilize this geneme information for improved breeding of this species . We fond specific loci responsible for each phenotypes，such as body size, scale development, resistance against a specific parasite, and behavioral patterns, using inter-species hybridization among Takifugu species. In addition, we found sex determination gene, and developed sex control system of this species, in which male is more valuable.

Research Products

• [Journal Article] Genomic regions ofpufferfishes responsible for host specificity of a monogenean parasite Heterobothrium okamotoi (2013)
  • Author(s): Hosoya,S., Kido,S., Hirabayashi,Y., Kai,W., Kinami,R., Yoshinaga,T., Ogawa,K., Suetake,H., Kikuchi,K., and Suzuki,Y.
  • Journal Title: Intl. J. Parasitol
• [Journal Article] The genetic architecture of growth rate in juvenile Takifugu species (2013)
  • Author(s): Hosoya, S., Kai, W., Fujita, M., Miyaki, K., Suetake, H., Suzuki, Y., and Kikuchi, K.
  • Journal Title: Evolution Volume: 67 Pages: 590-598
  • DOI: doi:10.1111/j.1558-5646.2012.01781.x
• [Journal Article] A Trans-species Missense SNP in Amhr2 is Associated with Sex Determination in the Tiger Pufferfish, Takifugu rubripes (fugu) (2012)
  • Author(s): Kamiya, T., Kai, W., Tasumi, S., Oka, A., Matsunaga, T., Mizuno, N., Fujita, M., Suetake, H., Suzuki, S., Hosoya, S., Tohari, S., Brenner, S., Miyadai, T., Venkatesh, B., Suzuki, Y., and Kikuchi, K.
  • Journal Title: PLoS Genet Volume: 8 Pages: e1002798
  • DOI: doi:10.1371/journal.pgen.1002798
• [Journal Article] トラフグのゲノム地図の作製とその応用-性決定遺伝子,性統御,比較ゲノム解析を中心に- (2012)
  • Author(s): 菊池潔・甲斐渉・細谷将・田角聡志・末武弘章・宮台俊明・鈴木譲
  • Journal Title: 水産育種 Volume: 41 Pages: 141-151
• [Journal Article] Integration of the Genetic Map and Genome Assembly of Fugu Facilitates Insights into Distinct Features of Genome Evolution in Teleosts and Mammals (2011)
  • Author(s): Kai,W., Kikuchi,K., Tohari,S., Chew,S.K., Tay,A., Fujiwara,A., Hosoya,S., Suetake,H., Naruse,K., Brenner,S., Suzuki,Y., and Venkatesh,B.
  • Journal Title: Genome Biology and Evolution Volume: 3 Pages: 424-442
  • DOI: doi:10.1093/gbe/evr041
• [Journal Article] Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish (2011)
  • Author(s): Toda,H., Saito,Y., Koike,T., Takizawa,F., Araki,K., Yabu,T., Somamoto,T., Suetake,H., Suzuki,Y., Ototake,M., Moritomo,T., and Nakanishi,T.
  • Journal Title: Dev. Comp. Immunol Volume: 35 Pages: 650-660
  • DOI: doi:10.1016/j.dci.2011.01.013
• [Journal Article] The plasmablast-like leukocyte in the kidney of fugu (Takifugu rubripes) (2011)
  • Author(s): Odaka,T., Tsutsui,S., Sugamata,R., Suetake,H., Miyadai,T., Suzuki,Y., Watanabe,T., and Nakamura,O.
  • Journal Title: Fish Shellfish Immunol Volume: 30 Pages: 682-690
  • DOI: doi:10.1016/j.fsi.2010.12.018
• [Journal Article] フグ類の多様化をもたらした遺伝子の探索-種間交雑を利用したゲノムワイドマッピング- (2010)
  • Author(s): 菊池潔・甲斐渉・細谷将・末武弘章・鈴木譲
  • Journal Title: 月刊海洋 Volume: 42 Pages: 363-371
• [Journal Article] Teleost B7s expressed on monocytes regulate T cell responses (2009)
  • Author(s): Sugamata,R., Suetake,H., Kikuchi,K., and Suzuki,Y.
  • Journal Title: J. Immunol. Volume: 182 Pages: 6799-6806
  • DOI: doi/10.4049/jimmunol.0803371
• [Journal Article] Individual variations in behavior and free cortisol responses to acute stress in tiger pufferfish (Takifugu rubripes) (2008)
  • Author(s): Hosoya,S., Kaneko,T., Suzuki,Y. and Hino,A.
  • Journal Title: Fisheries Sci Volume: 74 Pages: 755-763
  • DOI: doi:10.1111/j.1444-2906.2008.01586.x
• [Journal Article] Characterization ofCD8-positive leukocytes in fugu (Takifugu rubripes) with antiserum against fugu CD8α (2008)
  • Author(s): Araki,K., Akatsu,K., Suetake,H., Kikuchi,K., and Suzuki,Y.
  • Journal Title: Dev. Comp. Immunol Volume: 32 Pages: 850-858
  • DOI: doi:10.1016/j.dci.2007.12.005
• [Journal Article] トラフグのゲノムマップ計画と魚類養殖におけるゲノム情報の利用法 (2008)
  • Author(s): 菊池潔,甲斐渉,末武弘章,鈴木譲
  • Journal Title: 水産育種 Volume: 38 Pages: 25-30
• [Journal Article] ゲノムデータベースを利用したトラフグ優良形質遺伝子の探索 (2008)
  • Author(s): 渡部終五・木下滋晴・池田大介・古川聡史・鈴木譲・菊池潔・甲斐渉
  • Journal Title: 水研センター研報 Volume: 26 Pages: 83-89
• [Presentation] 超雄トラフグ(YY型魚)の作出とその生存率 (2013)
  • Author(s): 菊池潔・野澤碧・城夕香・藤田真志・水野直樹・濱崎将臣・鈴木重則・田角聡志・鈴木譲
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20130326-30
• [Presentation] トラフグの雄型性決定遺伝子はクサフグを雄化する (2013)
  • Author(s): 家田梨櫻・穴井康崇・藤田真志・渥美九郁・田島祥太・中村修・田角聡志・鈴木譲・菊池潔
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20130326-30
• [Presentation] 連続戻し交雑家系を用いたトラフグ"噛み合い"遺伝子座の探索 (2013)
  • Author(s): 細谷将・丹羽悠二・水野直樹・鈴木譲・菊池潔
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20130326-30
• [Presentation] トラフグとクサフグの鰓において存在量の異なる膜タンパク質の単離 (2013)
  • Author(s): 田角聡志・山口晶・筒井繁行・中村修・菊池潔・鈴木譲
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20130326-30
• [Presentation] A fish chemokine expressed in the secondary lymphoid organs in fugu Takifugu rubripes (2012)
  • Author(s): Tauchi, H., Suetake, H., Odaka, T., Kaneda, M., Maeda, T., Kikuchi, K., Suzuki, Y., and Miyadai, T.
  • Organizer: 12^<th> Congress of ISDCI
  • Place of Presentation: Fukuoka
  • Year and Date: 20120709-13
• [Presentation] Candidate key molecule(s) determining host specificity of parasite on the pufferfish,Takifugu (2012)
  • Author(s): Tasumi, S., Yamaguchi, A.,Hirabayashi, Y., Kido, S., Kobayashi, K.,Kai, W., Hosoya, S., Tsutsui, S.,Nakamura, O., Suetake, H., Kikuchi, K.and Suzuki, Y.
  • Organizer: 12^<th> Congress of ISDCI
  • Place of Presentation: Fukuoka
  • Year and Date: 20120709-13
• [Presentation] 遡河回遊のゲノムワイド遺伝解析にむけて--河にさかのぼるフグと海産フグの遺伝学-- (2012)
  • Author(s): 菊池潔・濱崎将臣・宮木廉夫・土井啓行・合田知樹・藤田真志・鈴木譲
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 下関
  • Year and Date: 2012-09-15
• [Presentation] Genetic Basis of Parallel Evolution of Scale Loss in Takifugu Pufferfishes (2011)
  • Author(s): Yamanoue,Y., Kai, W., Kuroyanagi, W. Hosoya, S., Suetake, H., Suzuki, Y., and Kikuchi, K.
  • Organizer: Annual Meeting of the Society for Molecular Biology and Evolution
  • Place of Presentation: Kyoto
  • Year and Date: 20110726-30
• [Presentation] フグのゲノム地図作成と性統御および育種への応用.日本水産学会シンポジウム「水産育種におけるゲノム情報利用の現状と将来展望」 (2011)
  • Author(s): 菊池潔・甲斐渉
  • Organizer: 平成23年度日本水産学会春季大会講演要旨集
  • Year and Date: 20110300
• [Presentation] トラフグのゲノム育種.日本水産学会シンポジウム「フグ研究とトラフグ生産技術開発の最前線」 (2011)
  • Author(s): 鈴木譲・菊池潔
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎
  • Year and Date: 2011-10-02
• [Presentation] 近縁種間で脊椎骨数の差をもたらす遺伝子座 (2011)
  • Author(s): 合田知樹・細谷将・城夕香・菊池潔・鈴木譲
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎
  • Year and Date: 2011-09-30
• [Presentation] 寄生虫の宿主特異性に関わるフグ遺伝子の探索 (2011)
  • Author(s): 平林陽・木戸慎一・木南竜平・細谷将・甲斐渉・菊池潔・城夕香・末武弘章・良永知義・小川和夫・鈴木譲
  • Organizer: 日本比較免疫学会第23回学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-08-22
• [Presentation] Genetic Basis of Parallel Evolution for the Spiny Scale in the Rapid Radiation of Pufferfishes (2011)
  • Author(s): Kikuchi,K., Kai,W., Kuroyanagi,M., Nozawa,A., Hosoya,S., Suetake,H., and Suzuki,Y.
  • Organizer: The 20th CDB Meeting Molecular Bases for Evolution of Complex Traits
  • Place of Presentation: Kobe
  • Year and Date: 2011-02-23
• [Presentation] Genetic architecture of interspecific differences in growth of pufferfishes (2010)
  • Author(s): Hosoya,S., Kikuchi,K., Kai,W., Mizuno,N., Suetake,H., and Suzuki,Y.
  • Organizer: 9th International Congress for the Biology of Fish
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-05
• [Presentation] Fuguversion5-トラフグのゲノム地図- (2010)
  • Author(s): 甲斐渉・菊池潔・末武弘章・鈴木譲
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 神奈川
  • Year and Date: 2010-03-29
• [Presentation] フグ類の多様化をもたらした遺伝子の探索:種間交雑を利用したゲノムワイドQTL解析 (2009)
  • Author(s): 菊池潔・甲斐渉・細谷将・木戸慎一・末武弘章・鈴木譲
  • Organizer: 2009年度日本魚類学会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-10-12
• [Presentation] Genetic approach foridentifying the genes responsible for resistance and susceptibility against a parasite, Heterobothrium okamotoi, in fugu, Takifugu rubripes (2009)
  • Author(s): Kido,S., Kai,W., Suetake,H., Kikuchi,K., and Suzuki,Y.
  • Organizer: 11th Congress of ISDCI
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2009-07-03
• [Presentation] Teleost B7 molecules on monocytes regulate T cell responses (2009)
  • Author(s): Sugamata R., Suetake H., Jo Y., Kikuchi K., and Suzuki Y.
  • Organizer: 11th Congress of International Society of Developmental and Comparative Immunology
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2009-06-29
• [Presentation] Fugu genome map and its application in aquaculture 5th World Fisheries Congress (2008)
  • Author(s): Kikuchi,K., Kai,W., Kido,S., Kamiya,T., Hosoya,S., Suetake,H., and Suzuki,Y.
  • Place of Presentation: Yokohama
  • Year and Date: 20081020-24
• [Presentation] トラフグのゲノム解読が魚類養殖を変える (2008)
  • Author(s): 鈴木譲
  • Organizer: 第5回東京大学の生命科学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-04-19
• [Book] トラフグのゲノム育種.長島裕二・村田修・渡部終五編「フグ研究とトラフグ生産技術の最前線」 (2012)
  • Author(s): 鈴木譲・菊池潔
  • Total Pages: 69-83
  • Publisher: 恒星社厚生閣
• [Remarks] Fifth Genome Assembly(IMCBのサイト)
  • URL: http://www.fugu-sg.org/
• [Remarks] トラフグ連鎖地図
  • URL: http://www.se.a.u-tokyo.ac.jp/Fugu-Map/map.html