骨軟骨移植術および関節軟骨損傷のリハビリテーションに関する基礎的研究

2009 Fiscal Year Annual Research Report

• Project/Area Number: 20240057
• Research Institution: Kyoto University
• Principal Investigator: 黒木 裕士 京都大学, 医学研究科, 教授 (20170110)
• Co-Investigator(Kenkyū-buntansha): 小林 雅彦 京都大学, 医学研究科, 講師 (20378623) ; 中川 泰彰 独立行政法人国立病院機構(京都医療センター臨床研究センター), 臨床研究企画研究科, 研究員 (90293860)
• Keywords: 骨軟骨移植術 / 関節軟骨 / リハビリテーション / 超音波 / 硬度

Research Abstract

骨軟骨移植術の動物実験においては、現在まで、術後荷重と運動が移植された骨軟骨プラグの軟骨に及ぼす影響についての報告がなされていない。そこで家兎モデルを作製して骨軟骨移植術において術後の荷重と運動開始が骨軟骨プラグの軟骨の超音波硬度指標、および関節可動域に及ぼす影響等を調べることを目的とした。平成21年度には家兎の膝関節軟骨に骨軟骨移植モデルを作り、移植軟骨の術後の経過を、肉眼的に、組織学的に、および免疫組織学的に観察した。また超音波解析とFTIR解析によって調べた。力学的解析はまだ実施できていない。具体的には、動物実験委員会の承認を得て家兎30羽に骨軟骨移植術を施した。現有のOATS(Arthrex,USA)を用い家兎の左膝蓋大腿関節の大腿関節面側に直径5mmのレシピエントホールを作った。右膝蓋大腿関節の大腿面側から直径6mmの骨軟骨プラグを採取してレシピエントホールに移植した。術後は家兎を荷重/非荷重の2種類の条件下で飼育した。術後一定期間経過後に家兎を安楽死させて膝関節標本を採取し、超音波装置を用いて採取した骨軟骨移植術後の骨軟骨プラグの軟骨の硬度指標、表面粗さ指標、厚さ指標を測定した。また採取標本を肉眼的および組織学的所見、ならびに免疫組織学的に比較検討した。加えてFTIR解析を行った。また国内外の学会等に出席して研究に必要な最新で最先端の情報・研究資料を収集した。

Research Products

• [Journal Article] Positive effect of alendronate on subchondral bone healing and subsequent cartilage repair in a rabbit osteochondral defect model (2009)
  • Author(s): Nishitani K, Shirai T, Kobayashi M, Kuroki H, Azuma Y, Nakagawa Y, Nakamura T
  • Journal Title: American Journal of Sports Medicine Volume: 37 Pages: 139S-147S
  • Peer Reviewed
• [Journal Article] Ultrasound has the potential to detect degeneration of articular cartilage clinically, even if the information is obtained from an indirect measurement of intrinsic physical characteristics (2009)
  • Author(s): Kuroki H, Nakagawa Y, Mori K, Kobayashi M, Yasura K, Okamoto Y, Suzuki T, Nishitani K, Nakamura T.
  • Journal Title: Arthritis Research & Therapy Volume: 11 Pages: 408
  • Peer Reviewed
• [Presentation] Age-related behavior of collagen and proteoglycan in superficial, middle and deep zones of patellar cartilage : fourier transform infrared spectroscopy (FTIR) analysis of normal rabbits (2009)
  • Author(s): Kuroki H, Nakagawa Y, Kobayashi M, Mori K, Nishitani K, Shirai T, Satake T, Kobayashi-Miura M, Torii Y, Tsuchimoto K, Nakamura S, Arai R, Nakamura T.
  • Organizer: The 2009 World Congress on Osteoarthritis
  • Place of Presentation: Montreal
  • Year and Date: 2009-09-11
• [Presentation] Effect of Alendronate on articular cartilage and subchondral bone changes in a rabbit model of osteoarthritis (2009)
  • Author(s): Shirai T, Kobayashi M, Nishitani K, Satake T, Kuroki H, Nakagawa Y, Nakamura T.
  • Organizer: The 2009 World Congress on Osteoarthritis
  • Place of Presentation: Montreal
  • Year and Date: 2009-09-11
• [Presentation] The ultrasound can evaluate macroscopically undetectable cartilage degeneration in rabbit osteoarthritis model (2009)
  • Author(s): Nishitani K, Kobayashi M, Shirai T, Satake T, Kuroki H, Mori K, Nakagawa Y, Nakamura T.
  • Organizer: The 2009 World Congress on Osteoarthritis
  • Place of Presentation: Montreal
  • Year and Date: 2009-09-11
• [Presentation] Cartilage damage severity predicts clinical results after partial meniscectomy for horizontal degenerative tear of medical meniscus (2009)
  • Author(s): Kobayashi M, Nakamura S, Arai R, Nishitani K, Shirai T, Satake T, Mizuno Y, Furukawa T, Kuroki H, Nakagawa Y, Nakamura T.
  • Organizer: The 2009 World Congress on Osteoarthritis
  • Place of Presentation: Montreal
  • Year and Date: 2009-09-11
• [Presentation] Can signal intensity of ultrasound detect fragility of superficial layer of articular cartilage-like tissue?: change over time up to 1-year (2009)
  • Author(s): Kuroki H, Nakagawa Y, Mori K, Kobayashi M, Nishitani K, Shirai T, Satake T, Nakamura S, Arai R, Nakamura T.
  • Organizer: ICRS 2009, the 8th World Congress of the International Cartilage Repair Society
  • Place of Presentation: Miami
  • Year and Date: 2009-05-25
• [Presentation] Importance of early bone remodeling for cartilage regeneration in rabbit osteochondral defect model ; positive effect of Alendronate (2009)
  • Author(s): Kobayashi M, Nishitani K, Shirai T, Kuroki H, Mori K, Nakagawa Y, Satake T, Nakamura T.
  • Organizer: ICRS 2009, the 8th World Congress of the International Cartilage Repair Society
  • Place of Presentation: Miami
  • Year and Date: 2009-05-25
• [Presentation] Operative outcome of mosaicplasty with no procedure in exposing subchondral bone of tibia (2009)
  • Author(s): Nakagawa Y, Kobayashi M, Nakamura S, Kuroki H, Nakamura T.
  • Organizer: ICRS 2009, the 8th World Congress of the International Cartilage Repair Society
  • Place of Presentation: Miami
  • Year and Date: 2009-05-25
• [Presentation] Osteochondral autologous transfer for an early osteoarthritis of knee joint (2009)
  • Author(s): Kobayashi M, Nakagawa Y, Nakamura S, Arai R, Nishitani K, Shirai T, Satake T, Kuroki H, Nakamura T.
  • Organizer: ICRS 2009, the 8th World Congress of the International Cartilage Repair Society
  • Place of Presentation: Miami
  • Year and Date: 2009-05-25
• [Presentation] A comparison of ultrasound properties of articular cartilage immediately after osteochondral grafting surgery : In cases of traumatic cartilage lesions and osteonecrosis (2009)
  • Author(s): Kuroki H, Nakagawa Y, Mori K, Kobayashi M, Nakamura S, Nishitani K, Shirai T, Nakamura T.
  • Organizer: The 7th ISAKOS Congress
  • Place of Presentation: Osaka
  • Year and Date: 2009-04-05
• [Presentation] Osteochondral Autogenous transfer for an early stage of knee osteoarthritis. The surgical outcome of the osteochondral grafts for knee osteoarthritis (2009)
  • Author(s): Nakagawa Y, Kobayashi M, Okudaira S, Nakamura S, Kuroki H, Nakamura T.
  • Organizer: The 7th ISAKOS Congress
  • Place of Presentation: Osaka
  • Year and Date: 2009-04-05
• [Presentation] Mechanical damage and chemical contaminants on reprocessed arthroscopic shaver blades (2009)
  • Author(s): Kobayashi M, Nakamura T, Nakagawa Y, Okamoto Y, Nakamura S, Nishitani K, Shirai T, Arai R, Satake T, Kuroki H.
  • Organizer: The 7th ISAKOS Congress
  • Place of Presentation: Osaka
  • Year and Date: 2009-04-05