放射線、化学物質の継世代的(遺伝的)影響-マイクロサテライト突然変異誘発と疾病

2010 Fiscal Year Final Research Report

• Project/Area Number: 20241014
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: National Institute of Biomedical Innovation
• Principal Investigator: NOMURA Taisei National Institute of Biomedical Innovation, 難病・疾患資源研究所, プロジェクトリーダー (90089871)
• Co-Investigator(Kenkyū-buntansha): RYO Haruko 独立行政法人医薬基盤研究所, プロジェクトサブリーダー (90301267) ; ADACHI Shigeki 独立行政法人医薬基盤研究所, 特任研究員 (60379261) ; FUJIKAWA Kazuo 近畿大学, 理工学部, 教授 (90247958) ; HONGYO Tadashi 大阪大学, 大学院・医学系研究科, 准教授 (90271569) ; NAKAJIMA Hiroo 大阪大学, 大学院・医学系研究科, 助教 (20237275) ; OKUMURA Meinosin 大阪大学, 大学院・医学系研究科, 教授 (40252647)
• Project Period (FY): 2008 – 2010
• Keywords: 放射線・化学物質 / 継世代影響 / マイクロサテライト / 疾病 / 遺伝子発現 / 中性子線 / 白血病 / N5マウス

Research Abstract

Microsatellite mutations increased almost linearly in the F1 offspring of adult N5 males exposed to nuclear radiations (neutron 0.2Gy/h + γ-rays 0.2Gy/h) at spermatogonia stages. Mutation was inherited as if Menderian inheritance. Its rate was 3.5 x 10^<-2>/Gy/locus and RBE was about 16. Leukemia and changes in gene expression were also inherited in the offspring.

Research Products

• [Journal Article] A rapid and easy method for the qualitative detection of intracellular 2 deposition of inhaled nanoparticles Nanomedicine (2011)
  • Author(s): H.Nakajima, K.Ozaki, T.Hongyo, I.Narama, T.Todo
  • Journal Title: E.Pub.
  • Peer Reviewed
• [Journal Article] Measurement of relative biological effectiveness of protons in human cancer cells using a laser-driven quasimonoenergetic proton beamline. (2011)
  • Author(s): Yogo A.(1番目), Nomura T.(17番目), Kondo K.(19番目)(19名)
  • Journal Title: APPLIED PHYSICS LETTERS 98 Pages: 053701
  • Peer Reviewed
• [Journal Article] Biological Consequence and Health Concern from Low Dose and Low Dose Rate Radiations in Mice and Humans. (2011)
  • Author(s): Taisei Nomura.
  • Journal Title: Health Physics. 100 Pages: 266-268
  • Peer Reviewed
• [Journal Article] Excretion into feces of asialo GM1 in the murine digestive tract and Lactobacillus johnsonii exhibiting binding ability toward asialo GM1. A possible role of epithelial glycolipids in the discharge of intestinal bacteria. (2011)
  • Author(s): Iwamori M, Iwamori Y, Adachi S, Nomura T.
  • Journal Title: Glycoconj. J. 28 Pages: 21-30
  • Peer Reviewed
• [Journal Article] Effects of Fission Neutrons on Human Thyroid Tissues Maintained in SCID Mice. (2010)
  • Author(s): S.Adachi, H.Ryo, T.Hongyo, H.Nakajima, R.Tsuboi-Kikuya, Y.Tokita, F.Matsuzuka, K.Hiramatsu, K.Fujikawa, T.Itoh, T.Nomura
  • Journal Title: Mutat Res. 696 Pages: 107-113
  • Peer Reviewed
• [Journal Article] No evidence of increased mutation rates at microsatellite loci in offspring of A-bomb survivors. (2010)
  • Author(s): M.Kodaira, H.Ryo, N.Kamada, K.Furukawa, N.Takahashi, H.Nakajima, T.Nomura, N.Nakamura
  • Journal Title: Radiat.Res. 173 Pages: 205-213
  • Peer Reviewed
• [Journal Article] Clinical outcome of resected solid-type small-sized c-stage IA non-small cell lung cancer (2010)
  • Author(s): Inoue M , Minami M, Sawabata N, Utsumi T, Kadota Y, Shigemura N, Okumura M
  • Journal Title: Eur J Cardiothorac Surg. 37 Pages: 1445-9
  • Peer Reviewed
• [Journal Article] Radiation dose-rate effect on mutation induction in spleen and liver of gtp delta mice. (2010)
  • Author(s): Okudaira, N.(1番目), Fujikawa K(3番目), T. Ono(16番目)(16名)
  • Journal Title: Radiation Res. 171 Pages: 136-147
  • Peer Reviewed
• [Journal Article] Optimal sampling methods for margin cytology examination following lung excision (2009)
  • Author(s): Utsumi T , Sawabata N, Inoue M, Okumura M
  • Journal Title: Interact Cardiovasc Thorac Surg. 2010(10) Pages: 434-6
  • Peer Reviewed
• [Journal Article] Distribution of receptor glycolipids for Lactobacilli in murine digestive tract and production of antibodies cross-reactive with them by immunization of rabbits with Lactobacilli. (2009)
  • Author(s): Iwamori M, Shibagaki T, Nakata Y, Adachi S, Nomura T.
  • Journal Title: J Biochem. 146(2) Pages: 185-91
  • Peer Reviewed
• [Journal Article] Rapid diagnosis of lymph node metastasis in lung cancer with loop-mediated isothermal amplification assay using. (2009)
  • Author(s): Maeda J, Inoue M, Nakabayashi K, Otomo Y, Shintani Y, Ohta M, Okumura M, Matsuura N.
  • Journal Title: Carcinoembryonic antigen-mRNA Lung cancer 65 Pages: 324-327
  • Peer Reviewed
• [Journal Article] Microdosimetry on a mini-reactor UTR-KINKI for educational uses and biological researches (2009)
  • Author(s): Endo, S., K.Tanaka, K.Fujikawa, T.Horiguchi, T.Itoh, Y.Onizuka, M.Hoshi, A.Murakata, Y.Kojima, K.Shizuma
  • Journal Title: Journal of Radiation Research 50 Pages: 83-87
  • Peer Reviewed
• [Journal Article] Experimental Derivation of Relative Biological Effectiveness of A-Bomb Neutrons in Hiroshima and Nagasaki and Implications for Risk Assessment. (2008)
  • Author(s): Sasaki, M.S., Nomura, T., Ejima, Y., Utsumi, H., Endo, S., Saito, I., Itoh, T., Hoshi, M.
  • Journal Title: Radiat. Res. 170 Pages: 101-117
  • Peer Reviewed
• [Journal Article] Differential radiation sensitivity to morphological, functional and molecular changes of human thyroid tissues and bone marrow cells maintained in SCID mice. (2008)
  • Author(s): T.Nomura, T.Hongyo, H.Nakajima, L.Y.Li, M.Syaifudin, S.Adachi, H.Ryo, R.Baskar, K.Fukuda, Y.Oka, H.Sugiyama, F.Matsuzuka.
  • Journal Title: Mutat Res. 657(1) Pages: 68-76
  • Peer Reviewed
• [Journal Article] Trangenerational effects from exposure to environmental toxic substance. (2008)
  • Author(s): T.Nomura
  • Journal Title: Mutat.Res.Rev. 659 Pages: 185-193
  • Peer Reviewed
• [Journal Article] Nasal NK/T-cell lymphoma : epidemiology and pathogenesis. (2008)
  • Author(s): K.Aozasa, T.Katakuwa, T.Hongyo, W-I.Yang
  • Journal Title: Int J Hematol 87 Pages: 110-117
  • Peer Reviewed
• [Journal Article] Heterologous carcinosarcoma of Douglas' pouch with adenocarcinomas of the fallopian tube and the peritoneal cavity (2008)
  • Author(s): T.R.Kataoka, H.Shinoda, M.Yanagawa, S.Kamiura, Y.Nishizawa, T.Hongyo
  • Journal Title: Pathology 40(6) Pages: 641-645
  • Peer Reviewed
• [Journal Article] Clinicopathologic factors influencing postoperative prognosis in patients with smallsized adenocarcinoma of the lung. (2008)
  • Author(s): I noue M, Takakura T, Minami M, Shiono H, Utsumi T, Kadota Y, Nasu T, Aozasa K, Okumura M.
  • Journal Title: J.Thorac Cardiovas.Surg. 135 Pages: 830-836
  • Peer Reviewed
• [Journal Article] Comparative susceptibility of kidney and gillcells of medaka fish (Oryzias latipes) to spontaneous and X-ray-induced micronucleus formation (2008)
  • Author(s): Takai, A., A.Kagawa, K.Fujikawa
  • Journal Title: Gene and Environment 30 Pages: 48-52
  • Peer Reviewed
• [Presentation] Carcinogenicity of Urethane (Ethyl Carbamate) in Mice And Humans. (2010)
  • Author(s): Nomura T.
  • Organizer: 2nd Asian Conference on Environmental Mutagens
  • Place of Presentation: Pattaya, Thailand.
  • Year and Date: 20101215-20101218
• [Presentation] Biological Consequences and Health Risk from Low and Low Dose Rate Radiation Exposures in Mice and Humans. (2010)
  • Author(s): Nomura T.
  • Organizer: LOWRAD 2010
  • Place of Presentation: Barcelona, Spain.
  • Year and Date: 20101213-20101215
• [Presentation] Novel approach for detection of isolated tumor cells in pulmonary vein using negative selection method : morphological classification and clinical implications (2010)
  • Author(s): Okumura M
  • Organizer: EACTS Annual Meeting (24th)
  • Place of Presentation: Geneva, Swiss
  • Year and Date: 20100911-20100915
• [Presentation] The use of Active Hexose Co-related Compound in the Treatment of Head & Neck Cancer Patients. (2010)
  • Author(s): Nomura T.
  • Organizer: The 18th International Congress on Nutrition and Integrative Medicine.
  • Place of Presentation: Sapporo, Japan.
  • Year and Date: 20100724-20100725
• [Presentation] Biological Consequences and Health Concern from Low Dose and Low Dose Rate Radiations in Mice and Human Tissues. (2010)
  • Author(s): Nomura T.
  • Organizer: International workshop "Biological consequences and health risks of low-level exposure to ionizing radiation"
  • Place of Presentation: Richland, USA.
  • Year and Date: 20100503-20100505
• [Presentation] Transgenerational health concerns from radiation in mice and humans. (2009)
  • Author(s): Taisei Nomura.
  • Organizer: In : The 15^<th> Alexander Hollaender Course ; Genome-Environment Interactions and Genetic Toxicology
  • Place of Presentation: Astana, Kazakhstan.
  • Year and Date: 20090923-20090927
• [Presentation] Ecological decrease, biological concentration and genome stress of radionuclides in plants and animals after Chernobyl catastrophe. (2009)
  • Author(s): Nakajima H
  • Organizer: 10th International Conference on Environmental Mutagens (ICEM)
  • Place of Presentation: Firenze, Italy.
  • Year and Date: 20090820-20090825
• [Presentation] Somatic stem cells of Atm-deficient mice are hypersensitive to X-rays for mutation but resistant for cell killing (2009)
  • Author(s): Fujikawa, K.
  • Organizer: The 10th International Conference on Environmental Mutagens
  • Place of Presentation: Firenze (Italy).
  • Year and Date: 20090820-20090825
• [Presentation] Surgical margin recurrence in cases of sublobar resection for small sized peripheral lung cancer (2009)
  • Author(s): Okumura M
  • Organizer: World Congress of Lung Cancer (13th)
  • Place of Presentation: San Francisco, USA.
  • Year and Date: 20090731-20090804
• [Presentation] Isolated tumor cells in pulmonary vein blood of resected lung with non-small cell lung cancer: a pilot study of morphological qualification. (2009)
  • Author(s): Okumura M.
  • Organizer: World Congress of Lung Cancer (13th)
  • Place of Presentation: San Francisco, USA.
  • Year and Date: 20090731-20090804
• [Presentation] Dietary Modulation to Prevent Cancer and Malformation in Mice. In : Dietary Modulation of Xenobiotics Transport and Metabolism. (2009)
  • Author(s): Taisei Nomura.
  • Organizer: 3rd Asian Pacific Regional Meeting of The International Society for the Study of Xenobiotics (ISSX)
  • Place of Presentation: Bangkok, Thailand.
  • Year and Date: 20090510-20090512
• [Presentation] Trans-generational effects of Radiation and Chemicals on Mice and Men - Cancer, Malformation and Molecular Changes. In ; Mega-Science Symposium. (2009)
  • Author(s): Taisei Nomura.
  • Organizer: 96^<th> Indian Science Congress
  • Place of Presentation: Shillong, India.
  • Year and Date: 20090103-20090107
• [Presentation] Differential Sensitivity of Mice and Human Tissues to Radiation Sources. (2009)
  • Author(s): Taisei Nomura.
  • Organizer: In : HIBMC/PMRC Joint Meeting
  • Place of Presentation: Tatsuno, Japan.
  • Year and Date: 2009-12-15
• [Presentation] Transgenerational (hereditary) effects of radiation in mice and humans - molecular changes and cancer induction in the progeny. (2008)
  • Author(s): Taisei Nomura.
  • Organizer: 6^<th> LOW RAD International Conference
  • Place of Presentation: Lisbon, Portugal.
  • Year and Date: 20081127-20081229
• [Book] Transgenerational health concerns from radiation in mice and humans. Astana : Eurasian National University Press. In : Bersimbay RI, Au W, eds. Genome-environment interactions and genetic toxicology. (2010)
  • Author(s): Nomura T.
  • Total Pages: 19-23
  • Publisher: 15^<th> Alexander Hollaender Course
• [Book] 知っておいてほしいダウン症候群の成因、「ダウン症候群児・者のヘルスケアマネジメント」 (2010)
  • Author(s): 藤川和男(分担執筆)
  • Total Pages: 19-46
  • Publisher: かもがわ出版
• [Book] ショウジョウバエの再発見 (2008)
  • Author(s): 藤川和男
  • Total Pages: 182
  • Publisher: サイエンス社
• [Remarks] 報道関連情報
• [Remarks] 野村大成、東奥日報、神戸新聞他20紙以上、「識者評論:農産物放射線汚染」、内部被曝の防止必要、正確な測定値と説明を、他、3月22日、23日 2011年。
• [Remarks] 野村大成、日経産業新聞、薬事日報 他4紙、「レーザー駆動陽子線、医薬基盤研などがん治療へ実験」 2010年6月29日-7月9日朝刊
• [Remarks] 野村大成 産経新聞(夕刊)2009年11月26日 "2012年宇宙の旅-三菱重が衛星計画- 任務 新薬開発のため無重力に耐えよ"
• [Remarks] 野村大成 日刊薬業 2009年11月27日"基盤研 創薬研究で来年度から新プロジェクト"
• [Remarks] 野村大成 日経産業 2009年11月30日"疾患モデル動物開発参加 食品なども対象に"