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2011 Fiscal Year Final Research Report

A modular strategy to construct functional RNA-protein complexes

Research Project

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Project/Area Number 20241051
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionKyoto University

Principal Investigator

MORII Takashi  京都大学, エネルギー理工学研究所, 教授 (90222348)

Co-Investigator(Kenkyū-buntansha) TAINAKA Kazuki  京都大学, エネルギー理工学研究所, 助教 (80506113)
Project Period (FY) 2008 – 2011
KeywordsRNA / ペプチド / インビトロセレクション / リセプター / センサー / 分子認識
Research Abstract

A stable complex of a peptide and RNA, ribonucleopeptide(RNP), provides a new framework to construct a macromolecular receptor for small molecules. The RRE RNA and the Rev peptide form a structurally well-characterized stable RNP complex that is suitable for a stepwise functionalization. In vitro selection of the RNP pool originating from an RRE-based RNA library and the Rev peptide affords RNP receptors specific for nucleotide triphosphates, for a phosphotyrosine residue in defined amino acid sequence, and for dopamine. A new ATP-binding motif for the ATP-binding RNP was characterized by a combination of biochemical and NMR studies. The RNP receptor functionalized by a fluorophore-labeled Rev peptide exerts optical signals associated with the ligand binding events. Replacing the Rev peptide of the ATP-binding RNP with a fluorophore-modified Rev peptide affords a series of fluorescent ATP sensors. This strategy to generate tailor-made fluorescent sensors is applied for a selective detection of a specific phosphorylated tyrosine residue within a defined amino acid sequence.

  • Research Products

    (23 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (10 results) Presentation (11 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Positional effects of phosphory lation on the stability and morphology of tau-related amyloid fibrils2012

    • Author(s)
      T. Morii, et al
    • Journal Title

      Biochemistry

      Volume: Vol.51, No.7 Pages: 1396-1406

  • [Journal Article] Zinc-Finger Proteins for Site-Specific Protein Positioning on DNA-Origami Structures2012

    • Author(s)
      T. Morii, et al
    • Journal Title

      Zinc-Finger Proteins for Site-Specific Protein Positioning on DNA-Origami Structures

      Volume: Vol.51, No.10 Pages: 2421-2424

  • [Journal Article] A ribonucleo peptide module for effective conversion of an RNA aptamer to a fluorescent sensor2011

    • Author(s)
      T. Morii, et al
    • Journal Title

      Bioorg. Med. Chem

      Volume: Vol.19, No.19 Pages: 5771-5775

  • [Journal Article] Construction of Dopamine Sensors by Using Fluorescent Ribonucleo peptide Complexes2011

    • Author(s)
      T. Morii, et al
    • Journal Title

      Bioorg. Med. Chem

      Volume: Vol.19, No.15 Pages: 4473-4481

    • DOI

      DOI:10.1016/j.bmc.2011.06.031

  • [Journal Article] Facile conversion of RNA aptamers to modular fluorescent sensors with tunable detection wavelengths2011

    • Author(s)
      T. Morii, et al
    • Journal Title

      Bioorg. Med. Chem. Lett

      Volume: Vol.21, No.15 Pages: 4503-4506

    • DOI

      DOI:10.1016/j.bmc1.2011.05.120

  • [Journal Article] Structural Aspects for the Recognition of ATP by Ribonucleopeptide Receptors2010

    • Author(s)
      T. Morii, et al
    • Journal Title

      J. Am. Chem. Soc

      Volume: Vol.133 Pages: 4567-4579

    • DOI

      DOI:10.1021/ja110725d

  • [Journal Article] An in vitro fluorescent sensor reveals intracellular Ins(1, 3, 4, 5) P4 dynamics in single cells2010

    • Author(s)
      T. Morii, et al
    • Journal Title

      Angew Chem. Mt. Ed

      Volume: Vol.49 Pages: 2150-2153

    • DOI

      DOI:10.1002/anie.200903951

  • [Journal Article] A Single Circularly Permuted GFP Sensor for Inosito1-1, 3, 4, 5-tetrakisphosphate Based on a Split PH Domain2009

    • Author(s)
      T. Morii, et al
    • Journal Title

      Bioorg. Med. Chem

      Volume: Vol.17 Pages: 7381-7386

    • DOI

      DOI:10.1016/j.bmc.2009.08.015

  • [Journal Article] Charge-Pairing Mechanism of Phosphorylation Effect upon Amyloid Fibrillation of Human Tau Core Peptide2008

    • Author(s)
      T. Morii, et al
    • Journal Title

      Biochemistry

      Volume: Vol.47 Pages: 11847-11857

    • DOI

      DOI:10.1021/bi8010994

  • [Journal Article] Context-dependent fluorescence detection of a phosphory lated tyrosine residue by a ribonucleo peptide

    • Author(s)
      T. Morii, et al
    • Journal Title

      J. Am. Chem. Soc

      Volume: (864)(1)

  • [Presentation] Assembling functional protein-nucleic acids complexes2012

    • Author(s)
      T. Morii
    • Organizer
      Dongguk University
    • Place of Presentation
      Korea
    • Year and Date
      2012-03-16
  • [Presentation] A Modular Strategy for Assembling Receptors and Sensors from Ribonucleopeptides2011

    • Author(s)
      T. Morii
    • Organizer
      2nd Asian 3 Round Table on Nucleic Acids
    • Place of Presentation
      China
    • Year and Date
      2011-10-15
  • [Presentation] Modular Functionalization of Ribonucleopeptide Assemblies2011

    • Author(s)
      T. Morii
    • Organizer
      School of Physical & Mathematical Sciences
    • Place of Presentation
      Singapore
    • Year and Date
      2011-04-13
  • [Presentation] Molecular Recognitiom by Ribonucleopeptides2010

    • Author(s)
      T. Morii
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      Hawaii
    • Year and Date
      2010-12-19
  • [Presentation] A Modular Strategy for Tailoring Fluorescent Biosensors from Ribonucleopeptides2010

    • Author(s)
      T. Morii
    • Organizer
      Asian 3 Roundtable on Nucleic Acids
    • Place of Presentation
      大阪
    • Year and Date
      2010-10-20
  • [Presentation] Selective Detection of Cellular Signaling Molecules.2010

    • Author(s)
      T. Morii
    • Organizer
      1st Asian Chemical Biology Conference
    • Place of Presentation
      Korea
    • Year and Date
      2010-06-27
  • [Presentation] Chemical approaches untangling dynamics of cellular signaling molecules, Kyoto-Oxford Univ2010

    • Author(s)
      T. Morii
    • Organizer
      Collaborative Forum
    • Place of Presentation
      U. K.
    • Year and Date
      2010-02-05
  • [Presentation] A Modular Strategy for Tailoring Fluorescent Sensors From Ribonucleopeptides2009

    • Author(s)
      T. Morii
    • Organizer
      3rd Asia-Pacific Int.
    • Place of Presentation
      Korea
    • Year and Date
      20091108-11
  • [Presentation] A Modular Strategy for Tailoring Functional Ribonucleo peptide Complexes2009

    • Author(s)
      T. Morii
    • Organizer
      2nd Swiss-Japan Biomolecular Chemistry Symposium 2009
    • Place of Presentation
      東京
    • Year and Date
      20090911-12
  • [Presentation] Design of Ribonucleopeptide-based Receptors and Fluorescent Sensors2008

    • Author(s)
      T. Morii
    • Organizer
      The IUMRS International Conference in Asia 2008
    • Year and Date
      20081209-13
  • [Presentation] A Modular Strategy for Tailoring Ribonucleopeptide-based Fluorescent Sensor2008

    • Author(s)
      T. Morii
    • Organizer
      Int. Sympo. On Molecular Recognition of DNA : Biological Applications
    • Year and Date
      2008-09-18
  • [Remarks] ホームページ

    • URL

      http://www.iae.kyoto-u.ac.jp/materia1/a-12_j.htm

  • [Patent(Industrial Property Rights)]2011

    • Inventor(s)
      森井孝,中田栄司,松本桂彦
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      特許、特願2011-244709
    • Filing Date
      2011-11-08

URL: 

Published: 2013-07-31  

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