Multidisciplinary studies on the biological functions of GnRH peptidergic neurons

2013 Fiscal Year Final Research Report

• Project/Area Number: 20247005
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: The University of Tokyo
• Principal Investigator: OKA Yoshitaka 東京大学, 理学(系)研究科(研究院), 教授 (70143360)
• Co-Investigator(Renkei-kenkyūsha): AKAZOME Yasuhisa 東京大学, 大学院理学系研究科, 助教 (50302807) ; KANDA Shinji 東京大学, 大学院理学系研究科, 助教 (50634284)
• Project Period (FY): 2008-04-08 – 2013-03-31
• Keywords: ペプチド / 生殖 / 性行動 / 神経生理学 / トランスジェニック / メダカ

Research Abstract

We generated GnRH1, GnRH2, and GnRH3 GFP-transgenic medaka to visualize each neuron in a whole brain in vitro preparation. We recorded spontaneous electrical activities and responses to various neurotransmitter/neuromodulator substances and analyzed their physiological functions. We also generated a transgenic medaka for monitoring the release activity of pituitary gonadotropic hormones and analyzed the central mechanisms of hormone release. Our research achievements enabled us to understand the multiple biological functions of GnRH peptidergic neurons that are based on their evolutionary history.

Research Products

• [Journal Article] Neurobiological study of fish brains gives insights into the nature of gonadotropin-releasing hormone 1-3 neurons (2013)
  • Author(s): Karigo, T., and Oka, Y.
  • Journal Title: Frontiers in Endocrinology (Experimental Endocrinology). "Biology of Gonadotropin-Releasing Hormone Neurons" Volume: 4:177(Article 177) Pages: 1-10
  • DOI: 10.3389/fendo.2013.00177
  • Peer Reviewed
• [Journal Article] Anatomical distribution of sex steroid hormone receptors in the brain of female medaka (2013)
  • Author(s): Zempo, B., Kanda, S., Okubo, K., Akazome, Y., and Oka, Y.
  • Journal Title: Journal of Comparative Neurology Volume: 521 Pages: 1760-1780
  • DOI: 10.1002/cne.23255
  • Peer Reviewed
• [Journal Article] Neuropeptide RFRP inhibits the pacemaker activity of terminal nerve GnRH neurons (2013)
  • Author(s): Umatani, C., Abe, H., and Oka, Y.
  • Journal Title: Journal of Neurophysiology Volume: 109 Pages: 2354-2363
  • DOI: 10.1152/jn.00145.2012
  • Peer Reviewed
• [Journal Article] Neuroanatomical evidence that kisspeptin directly regulates isotocin and vasotocin neurons (2013)
  • Author(s): Kanda, S., Akazome, Y., Mitani, Y., Okubo, K., and Oka, Y.
  • Journal Title: PLosOne Volume: 8 Pages: e62776
  • DOI: 10.1371/journal.pone.0062776
  • Peer Reviewed
• [Journal Article] Expression and putative function of kisspeptins and their receptors during early development in medaka (2013)
  • Author(s): Hodne, K., Weltzien, F.-A., Oka, Y., and Okubo, K.
  • Journal Title: Endocrinology Volume: 154 Pages: 3437-3446
  • DOI: 10.1210/en.2013-1065
  • Peer Reviewed
• [Journal Article] Burst generation mediated by cholinergic input in terminal nerve-gonadotrophin releasing hormone neurones of the goldfish (2013)
  • Author(s): Kawai, T., Abe, H., and Oka, Y.
  • Journal Title: Journal of Physiology-London Volume: 591 Pages: 5509-5523
  • DOI: 10.1113/jphysiol.2013.258343
  • Peer Reviewed
• [Journal Article] Evolutionary insights into the steroid sensitive kiss1 and kiss2 neurons in the vertebrate brain (2012)
  • Author(s): Kanda, S., and Oka, Y.
  • Journal Title: Frontiers in Genomic Endocrinology "Estrogenic control of hypothalamic GnRH neurons" Volume: 3:28(Article 28) Pages: 1-10
  • DOI: 10.3389/fendo.2012.00028
  • Peer Reviewed
• [Journal Article] Dopaminergic neuromodulation of synaptic transmission between the mitral and granule cells in the teleost olfactory bulb (2012)
  • Author(s): Kawai, T., Abe, H., and Oka, Y.
  • Journal Title: Journal of Neurophysiology Volume: 107 Pages: 1313-1324
  • DOI: 10.1152/jn.00536.2011
  • Peer Reviewed
• [Journal Article] Steroid sensitive kiss2 neurones in the goldfish: evolutionary insights into the duplicate kisspeptin gene-expressing neurones (2012)
  • Author(s): Kanda, S., Karigo, T., and Oka, Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 24 Pages: 897-906
  • DOI: 10.1111/j.1365-2826.2012.02296.x
  • Peer Reviewed
• [Journal Article] Time-of-day dependent changes in GnRH1 neuronal activities and gonadotropin mRNA expression in a daily spawning fish, medaka (2012)
  • Author(s): Karigo, T., Kanda, S., Abe, H., Okubo, K., and Oka, Y.
  • Journal Title: Endocrinology Volume: 153 Pages: 3394-3404
  • DOI: 10.1210/en.2011-2022
  • Peer Reviewed
• [Journal Article] Female-specific target sites for both estrogen and androgen in the teleost brain (2012)
  • Author(s): Hiraki, T., Takeuchi, A., Tsumaki, T., Zempo, B., Kanda, S., Oka, Y., Nagahama, Y., and Okubo, K.
  • Journal Title: Proceedings of the Royal Society. B, Biological sciences Volume: 279 Pages: 5014-5023
  • DOI: 10.1098/rspb.2012.2011
  • Peer Reviewed
• [Journal Article] Mechanisms of neuromodulation by a nonhypophysiotropic GnRH system controlling motivation for reproductive behavior in the teleost brain (2011)
  • Author(s): Abe, H., and Oka, Y.
  • Journal Title: Journal of Reproduction and Development Volume: 57 Pages: 665-674
  • Peer Reviewed
• [Journal Article] Sex differences in aromatase gene expression in the medaka brain (2011)
  • Author(s): Okubo, K., Takeuchi, A., Chaube, R., Paul-Prasanth, B., Kanda, S., Oka, Y., and Nagahama, Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 23 Pages: 412-423
  • DOI: 10.1111/j.1365-2826.2011.02120.x
  • Peer Reviewed
• [Journal Article] Expression of vesicular glutamate transporter-2.1 in medaka terminal nerve gonadotrophinreleasing hormone neurones (2011)
  • Author(s): Akazome, Y., Kanda, S., and Oka, Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 23 Pages: 570-576
  • DOI: 10.1111/j.1365-2826.2011.02142.x
  • Peer Reviewed
• [Journal Article] Differential regulation of luteinizing hormone genes in teleosts and tetrapods due to their distinct genomic environments-insights into gonadotropin beta subunit evolution (2011)
  • Author(s): Kanda, S., Okubo, K., and Oka, Y.
  • Journal Title: General and Comparative Endocrinology Volume: 173 Pages: 253–258
  • DOI: 10.1016/j.ygcen.2011.05.015
  • Peer Reviewed
• [Journal Article] Anatomical relations between neuropeptide Y, galanin, and gonadotropin-releasing hormone in the brain of chondrostean, the Siberian sturgeon Acipenser baeri (2011)
  • Author(s): Amiya, N., Amano, M., Tabuchi, A., and Oka, Y.
  • Journal Title: Neuroscience Letters Volume: 503 Pages: 87-92
  • DOI: 10.1016/j.neulet.2011.08.008
  • Peer Reviewed
• [Journal Article] Central distribution of kiss2 neurons and peri-pubertal changes in their expression in the brain of the male and female red seabream Pagrus major (2011)
  • Author(s): Shimizu, Y., Tomikawa, J., Hirano, K., Nanikawa, Y., Akazome, Y., Kanda, S., Kazeto, Y., Okuzawa, K., Uenoyama, Y., Ohkura, S., Tsukamura, H., Maeda, K.-I., Gen, K., Oka, Y., and Yamamoto, N.
  • Journal Title: General and Comparative Endocrinology Volume: 175 Pages: 432-442
  • DOI: 10.1016/j.ygcen.2011.11.038
  • Peer Reviewed
• [Journal Article] Functional and evolutionary insights into vertebrate kisspeptin systems from studies of fish brain (2010)
  • Author(s): Akazome, Y., Kanda, S., Okubo, K., and Oka, Y.
  • Journal Title: Special Issue of the Journal of Fish Biology; Reproductive Physiology STATE-OF-THE-SCIENCE, Journal of Fish Biology Volume: 76 Pages: 161-182
  • DOI: 10.1111/j.1095-8649.2009.02496.x
  • Peer Reviewed
• [Journal Article] Electrophysiological characteristics of gonadotrophinreleasing hormone 1-3 neurones: insights from a study of fish brains (2010)
  • Author(s): Oka, Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 22 Pages: 659-663
  • DOI: 10.1111/j.1365-2826.2010.02035.x
  • Peer Reviewed
• [Journal Article] Transgenic medaka in neurobiological studies (In Japanese) (2010)
  • Author(s): Kanda, S., and Oka, Y.
  • Journal Title: Laboratory Animal Technology and Science Volume: 22(5) Pages: 16-22
  • Peer Reviewed
• [Journal Article] Neurobiological mechanisms underlying GnRH pulse generation by the hypothalamus (2010)
  • Author(s): Maeda, K.-I., Ohkura, S., Uenoyama, Y., Wakabayashi, Y., Oka, Y., Tsukamura, H., and Okamura, H.
  • Journal Title: Brain Research 1364 (Review, Special Issue: New Insights into the Neurobiology of Reproduction and Puberty) Pages: 103-115
  • DOI: 10.1016/j.brainres.2010.10.026
  • Peer Reviewed
• [Journal Article] Regular pacemaker activity characterizes gonadotropinreleasing hormone 2 neurons recorded from green fluorescent protein-transgenic medaka (2010)
  • Author(s): Kanda, S., Nishikawa, K., Karigo, T., Okubo, K., Isomae, S, Abe, H., Kobayashi, D., and Oka, Y.
  • Journal Title: Endocrinology Volume: 151 Pages: 695-701
  • DOI: 10.1210/en.2009-0842
  • Peer Reviewed
• [Journal Article] Hypothalamic kiss1 but not kiss2 neurons are involved in estrogen feedback in medaka (Oryzias latipes) (2010)
  • Author(s): Mitani, Y., Kanda, S., Akazome, Y., Zempo, B., and Oka, Y.
  • Journal Title: Endocrinology Volume: 151 Pages: 1751–1759
  • DOI: 10.1210/en.2009-1174
  • Peer Reviewed
• [Journal Article] Excitatory action of GABA in the terminal nerve gonadotropinreleasing hormone neurons (2010)
  • Author(s): Nakane, R., and Oka, Y.
  • Journal Title: Journal of Neurophysiology Volume: 103 Pages: 1375-1384
  • DOI: 10.1152/jn.00910.2009
  • Peer Reviewed
• [Journal Article] Biochemical and immunohistochemical analyses of a GnRH-like peptide in the neural ganglia of the Pacific abalone Haliotis discus hannai (Gastropoda) (2010)
  • Author(s): Amano, M., Moriyama, S., Okubo, K., Nagai, Y., Amiya, N., Takahashi, A., and Oka, Y.
  • Journal Title: Zoological Science Volume: 27 Pages: 656–661
  • DOI: 10.2108/zsj.27.656
  • Peer Reviewed
• [Journal Article] Biochemical and immunohistochemical analyses of GnRH like peptides in the nerve ganglion of the chiton (2010)
  • Author(s): Amano, M., Yokoyama, T., Amiya, N., Hotta, M., Takakusaki, Y., Kado, R., and Oka, Y.
  • Journal Title: Acanthopleura japonica. Zoological Science Volume: 27 Pages: 924-930
  • DOI: 10.2108/zsj.27.924
  • Peer Reviewed
• [Journal Article] Electrophysiological analysis of the inhibitory effects of FMRFamide-like peptides on the pacemaker activity of gonadotropin-releasing hormone neurons (2010)
  • Author(s): Saito, T., Nakane, R., Akazome, Y., Abe, H., and Oka, Y.
  • Journal Title: Journal of Neurophysiology Volume: 104 Pages: 3518-3529
  • DOI: 10.1152/jn.01027.2009
  • Peer Reviewed
• [Journal Article] Neuromodulatory effect of GnRH on the synaptic transmission of the olfactory bulbar neural circuit in goldfish, Carassius auratus (2010)
  • Author(s): Kawai, T., Abe, H., Akazome, Y., and Oka, Y.
  • Journal Title: Journal of Neurophysiology Volume: 104 Pages: 3540-3550
  • DOI: 10.1152/jn.00639.2010
  • Peer Reviewed
• [Journal Article] Three types of gonadotrophin-releasing hormone neurones and steroid-sensitive sexually dimorphic kisspeptin neurones in teleosts (2009)
  • Author(s): Oka, Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 21 Pages: 334-338
  • DOI: 10.1111/j.1365-2826.2009.01850.x
  • Peer Reviewed
• [Journal Article] The role of the terminal nerve and GnRH in olfactory system neuromodulation (2009)
  • Author(s): Kawai, T., Oka, Y., and Eisthen, H.
  • Journal Title: Zoological Science Volume: 26 Pages: 669-680
  • DOI: 10.2108/zsj.26.669
  • Peer Reviewed
• [Journal Article] Primary culture of the isolated terminal nerve-gonadotrophin releasing hormone (GnRH) neurones derived from adult teleost (dwarf gourami, Colisa lalia) brain for the study of peptide release mechanisms (2009)
  • Author(s): Abe, H., and Oka, Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 21 Pages: 489-505
  • DOI: 10.1111/j.1365-2826.2009.01866.x
  • Peer Reviewed
• [Journal Article] Possible role of oestrogen in pubertal increase of Kiss1/Kisspeptin expression in discrete hypothalamic areas of female rats (2009)
  • Author(s): Takase, K., Uenoyama, Y., Inoue, N., Matsui, H., Yamada, S., Shimizu, M., Homma,T., Tomikawa, J., Kanda, S., Matsumoto, H., Oka, Y., Tsukamura, H., and Maeda, K.-I.
  • Journal Title: Journal of Neuroendocrinology Volume: 21 Pages: 527–537
  • DOI: 10.1111/j.1365-2826.2009.01868.x
  • Peer Reviewed
• [Journal Article] Interaction between neuropeptide Y immunoreactive neurons and galanin immunoreactive neurons in the brain of the masu salmon, Oncorhynchus masou (2009)
  • Author(s): Amano, M., Amiya, N., Hiramatsu, M., Tomioka, T., and Oka, Y.
  • Journal Title: Neuroscience Letters Volume: 462 Pages: 33-38
  • DOI: 10.1016/j.neulet.2009.06.067
  • Peer Reviewed
• [Journal Article] Calcium oscillations in the olfactory nonsensory cells of the goldfish, Carassius auratus (2009)
  • Author(s): Kawai, T., Abe, H., Wakabayashi, K., and Oka, Y.
  • Journal Title: Biochimica et Biophysica Acta-General Subjects Volume: 1790 Pages: 1681-1688
  • DOI: 10.1016/j.bbagen.2009.09.009
  • Peer Reviewed
• [Journal Article] Biochemical analysis and immunohistochemical examination of a GnRH-like immunoreactive peptide in the central nervous system of a decapod crustacean the kuruma prawn (Marsupenaeus japonicus) (2009)
  • Author(s): Amano, M., Okumura, T., Okubo , K., Amiya, N., Takahashi, A, and Oka, Y.
  • Journal Title: Zoological Science, Zoological Science Award. 2010 Volume: 26 Pages: 840-845
  • DOI: 10.2108/zsj.26.840
  • Peer Reviewed
• [Journal Article] Identification of KiSS-1 product kisspeptin and steroid-sensitive sexually-dimorphic kisspeptin neurons in medaka (Oryzias latipes) (2008)
  • Author(s): Kanda, S., Akazome, Y., Matsunaga, T., Yamamoto, N., Yamada, S., Tsukamura, H., Maeda, K., and Oka, Y.
  • Journal Title: Endocrinology Volume: 149 Pages: 2467-2476
  • DOI: 10.1210/en.2007-1503
  • Peer Reviewed
• [Journal Article] Coordinated synchronization in the electrically coupled network of terminal nerve gonadotropinreleasing hormone neurons as demonstrated by double patch-clamp study (2008)
  • Author(s): Haneda, K., and Oka, Y.
  • Journal Title: Endocrinology Volume: 149 Pages: 3540-3548
  • DOI: 10.1210/en.2008-0299
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of a GnRH-like peptide in the brain of the cephalopod spear-squid, Loligo bleekeri (2008)
  • Author(s): Amano, M., Oka, Y., Nagai, Y., Amiya, N., and Yamamori, K.
  • Journal Title: General and Comparative Endocrinol. Volume: 156 Pages: 277-284
  • DOI: 10.1016/j.ygcen.2008.01.008
  • Peer Reviewed
• [Journal Article] Identification and expression analysis of peroxisome proliferatoractivated receptors cDNA in a reptile, the leopard gecko (Eublepharis macularius) (2008)
  • Author(s): Kato, K., Oka, Y., and Park, M.K.
  • Journal Title: Zoological Science Volume: 25 Pages: 492-502
  • DOI: 10.2108/zsj.25.492
  • Peer Reviewed
• [Presentation] Morphological analysis of the early development of GnRH neuron systems in the EGFP-expressing transgenic medaka lines (2013)
  • Author(s): Takahashi, A., Islam, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
  • Organizer: The 43th Annual Meeting of the Society for Neuroscience 2013
  • Place of Presentation: San Diego
  • Year and Date: 20131109-13
• [Presentation] Neurobiological analysis of dopaminergic inhibition in the HPG axis regulation (2013)
  • Author(s): Karigo, T., Oka, Y.
  • Organizer: The 43th Annual Meeting of the Society for Neuroscience 2013
  • Place of Presentation: San Diego
  • Year and Date: 20131109-13
• [Presentation] 生殖の中枢制御に関わるペプチドニューロン系の研究 (2013)
  • Author(s): 岡良隆
  • Organizer: 日本動物学会第84回大会, 平成25年度日本動物学会賞受賞者講演
  • Place of Presentation: 岡山
  • Year and Date: 20130926-28
• [Presentation] 生殖・性行動調節にかかわる脳部位における性差 (2013)
  • Author(s): 岡良隆
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 20130926-28
• [Presentation] 終神経GnRHニューロンが視覚神経回路に及ぼす作用の解析 (2013)
  • Author(s): 馬谷千恵、阿部秀樹、岡良隆
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 20130926-28
• [Presentation] メダカの配偶者選択の分子神経基盤 (2013)
  • Author(s): 奥山輝大、横井佐織、阿部秀樹、磯江泰子、末廣勇司、今田はるか、島田敦子、川崎隆史、弓場俊輔、谷口善仁、亀井保博、田中実、成瀬清、武田洋幸、岡良隆、久保健雄、竹内秀明
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 20130926-28
• [Presentation] RFRP ニューロンの自発活動とGnRH1ニューロン発火抑制に関する電気生理学的解析 (2013)
  • Author(s): 貝瀬峻、神田真司、岡良隆
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 20130926-28
• [Presentation] 性ステロイド感受性を示すKiss1 ニューロンの電気生理学的解析 (2013)
  • Author(s): 長谷部政治、神田真司、島田洋之、岡良隆
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 20130926-28
• [Presentation] GnRH neurons involved in central regulation of reproduction and reproductive behavior (2013)
  • Author(s): Oka, Y.
  • Organizer: 2013 Gordon Research Conference, Neuroethology: Behavior, Evolution & Neurobiology; Networks, Circuits, and Modules
  • Place of Presentation: Mount Snow Resort, West Dover, VT, USA
  • Year and Date: 20130817-25
  • Invited
• [Presentation] 生殖の中枢制御の鍵を握るキスペプチンとその受容体GPR54を発現するニューロンへの新たな生理学的アプローチ (2013)
  • Author(s): 岡良隆
  • Organizer: 第10回GPCR 研究会
  • Place of Presentation: 東京
  • Year and Date: 20130510-11
  • Invited
• [Presentation] Functional and evolutionary diversity of vertebrate kisspeptin neuron systems 脊椎動物キスペプチン神経系の進化と多様な機能 (2013)
  • Author(s): Kanda, S. and Oka, Y.
  • Organizer: Hiroshi and Aya Irisawa Memorial Award Symposium, The 90th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Tokyo
  • Year and Date: 20130327-29
  • Invited
• [Presentation] 脊椎動物キスペプチン神経系の進化と未知の機能 (2013)
  • Author(s): 神田真司、岡良隆
  • Organizer: 東京大学大気海洋研究所共同利用研究集会
  • Place of Presentation: 柏
  • Year and Date: 2013-06-22
  • Invited
• [Presentation] gpr54-1-EGFP Transgenic Medaka - a New Approach towards the Understanding of Novel Functions of Kisspeptin Neurons (2012)
  • Author(s): Kanda, S., Nakajo, M., Lee, J., Oka, Y.
  • Organizer: The 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
  • Invited
• [Presentation] Embryonic Expression and Function of Kisspeptins and their Receptors in a Teleost Fish, medaka (2012)
  • Author(s): Okubo, K., Hodne, K., Kanda, S., Shimada, H., Oka, Y., Weltzien, F.A.
  • Organizer: The 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Electrophysiological and Morphological Analysis of Kiss1 Neurons in Trasgenic Medaka, Oryzias latipes (2012)
  • Author(s): Shimada, H., Kanda, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
  • Organizer: The 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Effects of Kisspeptins on LH Secretion and GnRH1 Neuronal Activities in Goldfish and Medaka (2012)
  • Author(s): Karigo, T., Uenoyama, Y., Oishi, S., Fujii, N., Mitani, Y., Kanda, S., Oka, Y.
  • Organizer: The 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Generation of kiss1 Knockout Medaka using Efficient New Genetic Tools, TALENs (2012)
  • Author(s): Takahashi, A., Kanda, S., Akazome, Y., Oka, Y.
  • Organizer: The 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Steroid Sensitive Hypothalamic Kiss1 Neurons in Medaka do not Co-express NKB or DYN (2012)
  • Author(s): Zempo, B., Kanda, S., Akazome, Y., Oka, Y.
  • Organizer: The 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Kiss2 may Activate Receptors for Neuropeptide FF (NPFF) / RF Amide Related Peptide (RFRP) in Medaka (2012)
  • Author(s): Akazome, Y., Yamamoto, E., Oka, Y.
  • Organizer: The 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Inhibitory Regulation of GnRH3 Neurons by RFRP Neurons in Medaka (2012)
  • Author(s): Nakane, R., Kanda, S., Oka, Y.
  • Organizer: The 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Neuroanatomical evidence for direct kisspeptin regulation of vasotocin and isotocin neurons (2012)
  • Author(s): S. Kanda, Y. Oka
  • Organizer: The 42th Annual Meeting of the Society for Neuroscience 2012
  • Place of Presentation: New Orleans, U.S.A.
  • Year and Date: 20121013-17
• [Presentation] Neuropeptide RFRP inhibits the pacemaker activity of extrahypothalamic GnRH neurons (2012)
  • Author(s): C. Umatani, H. Abe, Y. Oka
  • Organizer: The 42th Annual Meeting of the Society for Neuroscience 2012
  • Place of Presentation: New Orleans, U.S.A.
  • Year and Date: 20121013-17
• [Presentation] RFRPによる終神経GnRHニューロン発火活動抑制作用の解析 (2012)
  • Author(s): 馬谷千恵、阿部秀樹、岡良隆
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] 終神経GnRHニューロンにおけるコリン作動性入力を介したバースト活動の誘起 (2012)
  • Author(s): 河合喬文、阿部秀樹、岡良隆
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] 終神経GnRHペプチドニューロン細胞塊におけるGnRHニューロン間結合様式 (2012)
  • Author(s): 阿部秀樹、西川穂高、末次翔太、岡良隆
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] 生殖中枢制御に対するドーパミンを介した抑制作用に関する神経生物学的解析 (2012)
  • Author(s): 苅郷友美、岡良隆
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 20120913-15
• [Presentation] 絶食によるメダカ視床下部NPYニューロンのNPYmRNA発現変動 (2012)
  • Author(s): 北原翔一、神田真司、岡良隆
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 20120913-15
• [Presentation] キスペプチン受容体発現ニューロンのGFP標識による未知のキスペプチン機能の探索 (2012)
  • Author(s): 神田真司、李惇馥、岡良隆
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 20120913-15
• [Book] Structure, synthesis, and phylogeny of kisspeptin and its receptor. In: " Kisspeptin Signaling in Reproductive Biology " (ed. by A. Kauffman and J. Smith), Chapter 2, Adv Exp Med Biol. (2013)
  • Author(s): Kanda, S., and Oka, Y.
  • Total Pages: 784:9-26
  • Publisher: Springer Verlag, New York(doi:10.1007/978-1-4614-6199-9_2 査読有)
  • DOI: 10.1007/978-1-4614-6199-9_2
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/naibunpi/lab.html