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2011 Fiscal Year Final Research Report

MOLECULAR BASIS OF S-RNASE-BASED GAMETOPHYTIC SELF-INCOMPATIBILITY IN PRUNUS IN THE ROSACEAE

Research Project

  • PDF
Project/Area Number 20248004
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionKyoto University

Principal Investigator

TAO Ryutaro  京都大学, 大学院・農学研究科, 准教授 (10211997)

Co-Investigator(Kenkyū-buntansha) YAMANE Hisayo  京都大学, 大学院・農学研究科, 講師 (80335306)
HABU Tsuyoshi  京都大学, 大学院・農学研究科, 助教 (60335304)
Project Period (FY) 2008 – 2011
Keywordsアーモンド / アンズ,ウメ / オウトウ / 核果類 / スモモ / S-Rnase / SFB
Research Abstract

Prunus in the Rosaceae show the gametophytic self-incompatibility system, which hinders effective fruit production and breeding in Prunus. This study was started to elucidate the molecular basis of the gametophytic self-incompatibility in Prunus. We identified pollen expressed actin that binds to the pistil Sdeterminant, S-RNase. Furthermore, we identified a Skp1-like protein called SSK1 that binds to the pollen Sdeterminant, SFB. We have cloned Cul1 that forms the SCF complex with SSK1 and SFB. Based on the results obtained, we have developed the working hypothesis for the molecular recognition of the gametophytic self-incompatibility in Prunus.

  • Research Products

    (16 results)

All 2012 2011 2010 2009 2008

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (9 results)

  • [Journal Article] Identification of a Skp1-like protein interacting with SFB, the pollen Sdeterminant of the gametophytic self-incompatibility in Prunus2012

    • Author(s)
      Matumoto, D., H. Yamane, K. Abe, and R. Tao
    • Journal Title

      Plant Physiology

    • DOI

      DOI:10.1104/pp.112.197343

    • Peer Reviewed
  • [Journal Article] Isolation of pollen-expressed actin as a candidate protein interacting with S-RNase in Prunus aviumL.2012

    • Author(s)
      Matsumoto, D. and R. Tao.
    • Journal Title

      J. Japan. Soc. Hort. Sci.

      Volume: 81 Pages: 41-47

    • Peer Reviewed
  • [Journal Article] Molecular and genetic analyses of four non-functional Shaplotype variant derived from a common ancestral S haplotype identified in sour cherry (Prunus cerasusL.).2010

    • Author(s)
      Tsukamoto, T., N.R. Hauck, R. Tao, N. Jiang, and A.F. Iezzoni.
    • Journal Title

      Genetics

      Volume: 184 Pages: 411-427

    • Peer Reviewed
  • [Journal Article] Molecular basis of self-(in)compatibility and current status of S-genotyping in rosaceous fruit trees2009

    • Author(s)
      Yamane, H. and R. Tao.
    • Journal Title

      J. Japan. Hort. Sci

      Volume: 78 Pages: 137-157

    • Peer Reviewed
  • [Journal Article] A modifier locus affecting the expression of the S-RNase gene could be the cause of breakdown of self-incompatibility in almond. Sex2009

    • Author(s)
      Fernandez i Marti, A., T. Hanada, J.M. Alonso, H. Yamane, R. Tao, and R. Socias i Company.
    • Journal Title

      Plant Reprod.

      Volume: 22 Pages: 179-186

    • Peer Reviewed
  • [Journal Article] Cloning and characterization of a self-compatible Sfhaplotype in almond (Prunus dulcis(mill.) D. A. Webb. syn. P. amygladusBatsch) to ressolve previous confusion in its Sf-RNasesequence.2009

    • Author(s)
      Hanada, T., K. Fukuta., H. Yamane, T. Esumi, and R. Tao.
    • Journal Title

      HortScience

      Volume: 44 Pages: 609-613

    • Peer Reviewed
  • [Journal Article] Characterization of SLFL1, a pollen-expressed F-box gene located in the Prunus Slocus. Sex2008

    • Author(s)
      Matsumoto, D., H. Yamane, and R. Tao
    • Journal Title

      Plant Reprod

      Volume: 21 Pages: 113-121

    • Peer Reviewed
  • [Presentation] バラ科サクラ属における自家不和合性花粉側因子SFBとSCF複合体構成要素間の相互作用解析2012

    • Author(s)
      松本大生・田尾龍太郎
    • Organizer
      平成24年度園芸学会春季大会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス
    • Year and Date
      20120328-29
  • [Presentation] 不和合および和合受粉したウメ雌ずいのRNA-seq解析2012

    • Author(s)
      羽生剛・尾畑智哉・田尾龍太郎
    • Organizer
      平成24年度園芸学会春季大会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス
    • Year and Date
      20120328-29
  • [Presentation] カンカオウトウにおけるS-RNase結合花粉タンパク質としてのアクチンホモログの単離2011

    • Author(s)
      松本大生・田尾龍太郎
    • Organizer
      平成23年度園芸学会秋季大会
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      20110924-26
  • [Presentation] 育種・栽培化に伴うモモのS locusの進化2011

    • Author(s)
      赤木剛士・花田俊男・八重垣英明・山口正巳・田尾龍太郎
    • Organizer
      平成23年度園芸学会秋季大会
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      20110924-26
  • [Presentation] アーモンドの自家和合性に関わるSf ハプロタイプの解析2010

    • Author(s)
      田尾龍太郎・Fernandez, A. ・赤木剛士・花田俊男・Alonso, J.・Socias i Company, R
    • Organizer
      平成22年度園芸学会秋季大会
    • Place of Presentation
      大分大学旦野原キャンパス
    • Year and Date
      20100919-20
  • [Presentation] モモとモモ近縁種におけるSハプロタイプの共通性2009

    • Author(s)
      花田俊男・木部隆則・江角智也・山根久代・笹辺幸男・八重垣秀明・山口正己・Dandekar, A.M., Gradziel, T.M.・田尾龍太郎.
    • Organizer
      平成21年度園芸学会秋季大会
    • Place of Presentation
      秋田大学手形キャンパス
    • Year and Date
      20090926-28
  • [Presentation] Sハプロタイプの解析により示唆されたモモの自家和合化機構2009

    • Author(s)
      花田俊男・木部隆則・渡晶子・江角智也・山根久代・笹辺幸男・八重垣秀明・山口正己・Dandekar・A.M., Gradziel・T.M., Wunschi, A. ・田尾龍太郎
    • Organizer
      平成21年度園芸学会秋季大会
    • Place of Presentation
      秋田大学手形キャンパス
    • Year and Date
      20090926-28
  • [Presentation] モモにおける自家和合性Sハプロタイプのクローニングと塩基配列解析2009

    • Author(s)
      花田俊男・渡晶子・笹辺幸男・八重垣秀明・山口正己・田尾龍太郎
    • Organizer
      平成21年度園芸学会春季大会
    • Place of Presentation
      明治大学駿河台キャンパス
    • Year and Date
      20090319-20
  • [Presentation] サクラ属果樹における花粉側因子SFBと相互作用するSkp1の同定2008

    • Author(s)
      松本大生・山根久代・田尾龍太郎.
    • Organizer
      平成20年度園芸学会秋季大会
    • Place of Presentation
      三重大学生物資源学部
    • Year and Date
      20080927-29

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Published: 2014-08-29  

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