Analysis of multi-oscillator structure of biological clock

2010 Fiscal Year Final Research Report

• Project/Area Number: 20249010
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Hokkaido University
• Principal Investigator: HONMA Ken-Ichi Hokkaido University, 大学院・医学研究科, 特任教授 (40113625)
• Co-Investigator(Renkei-kenkyūsha): TANAHASHI Yuhsuke 北海道大学, 大学院・医学研究科, 助教 (50374228) ; NISHIDE Shinya 北海道大学, 大学院・医学研究科, 助教 (40451398) ; NAKAMUEA Tomoko 北海道大学, 大学院・医学研究科, 特任助教 (30451397) ; YAMADA Yoshiko 北海道大学, 大学院・医学研究科, 学術研究員 (10466451) ; YASUDA Tsubura 北海道大学, 大学院・医学研究科, 技術職員 (50399820) ; SHUDO Miwako 北海道大学, 大学院・医学研究科, 技術補助員 (20466452) ; YAMANAKA Yuhjiro 北海道大学, 大学院・医学研究科, 助教 (20528343) ; HASHIMOTO Satoko 北海道大学, 大学院・医学研究科, 博士研究員 (80374247) ; TOKUMARU Nobuko 北海道大学, 大学院・医学研究科, 博士研究員 (90567439)
• Project Period (FY): 2008 – 2010
• Keywords: 生物時計 / 時計遺伝子 / システム / 行動リズム / リズム同調

Research Abstract

Oscillating neurons in the suprachiasmatic nucleus (SCN), the center of biological clock, constructs more than one regional pacemakers of different characteristics through mutual synchronization. The roles of regional pacemakers depend on the area of SCN, and their interaction determines the length of activity period, showing seasonal changes in behavioral rhythms. The function of biological clock is disrupted by restricted daily feeding or methamphetamine treatment, which is supposed to be due to desynchronization between the central and peripheral clocks. The peripheral clock(s) regulating behavioral rhythms is suggested to reside in the midbrain.

Research Products

• [Journal Article] Physical exercise accelerates re-entrainment of human sleep-wake cycle but not of plasma melatonin rhythm to 8 h phase-advanced sleep schedule. (2010)
  • Author(s): Yamanaka Y., S.Hashimoto, Y.Tanahashi, S.Nishide, S.Honma, K.Honma
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 298 Pages: R681-R691
  • Peer Reviewed
• [Journal Article] Loss of circadian rhythm and light-induced suppression of pineal melatonin levels in Cry1 and Cry2 double-deficient mice. (2010)
  • Author(s): Yamanaka Y., Suzuki Y., Todo T, Honma K, Honma S.
  • Journal Title: Genes to Cells 10 Pages: 1063-1071
  • Peer Reviewed
• [Journal Article] The SCN-independent clocks, methamphetamine and food restriction. (2009)
  • Author(s): Honma K, Honma S.
  • Journal Title: Eur J Neurosci. 30 Pages: 1707-1717
  • Peer Reviewed
• [Journal Article] Effects of intracranial surgery on pineal lipid droplets, on other structures, and on melatonin secretion. (2009)
  • Author(s): Kuroshima M., G.Takahashi, T.Suzuki, S.Hashimoto, K.Honma, T.Kachi
  • Journal Title: Anal Sci Int 84 Pages: 17-26
  • Peer Reviewed
• [Journal Article] Liver X factors (LXRαand LXRβ) are potent regulators for hepatic DEC1 expression. (2009)
  • Author(s): Noshiro M., E.Usui, T.Kawamoto, F.Sato, A.Nakashima, T.Ueshima, K.Honda, K.Fujimoto, S.Honma, K.Honma, M.Makishima, Y.Kato
  • Journal Title: Genes to Cells 14 Pages: 29-40
  • Peer Reviewed
• [Journal Article] On the role of calcium and potassium currents in circadian modulation of firing rate in rat suprachiasmatic nucleus neurons : Multielectrode dish analysis. (2008)
  • Author(s): Kononenko N., S.Honma, F.D. Dudek, K.Honma
  • Journal Title: Neurosci.Research 62 Pages: 51-57
  • Peer Reviewed
• [Journal Article] The circadian pacemaker in the cultured suprachiasmatic nucleus from pup mice is highly sensitive to external perturbation. (2008)
  • Author(s): Nishide S., S.Honma, K.Honma
  • Journal Title: Eur J Neurosci 27 Pages: 2686-2690
  • Peer Reviewed
• [Journal Article] Scheduled exposures to a novel environment with a running-wheel differentially accelerate re-entrainment of mice peripheral clocks to new light-dark cycles. (2008)
  • Author(s): Yamanaka Y., S.Honma, K.Honma
  • Journal Title: Genes to Cells 13 Pages: 497-507
  • Peer Reviewed
• [Journal Article] Circadian behavioral rhythms in Cry1/Cry2 doubledeficient mice. (2008)
  • Author(s): Honma S., T.Yasuda, A.Yasui, G.T.J. van der Horst, K.Honma
  • Journal Title: J Biol Rhythm 23 Pages: 91-94
  • Peer Reviewed
• [Journal Article] A TTX sensitive local circuit is involved in the expression of PK2 and BDNF circadian rhythms in the mouse suprachiasmatic nucleus. (2008)
  • Author(s): Baba K., D.Ono, S.Honma, K.Honma
  • Journal Title: Eur J Neurosci 27 Pages: 909-916
  • Peer Reviewed
• [Presentation] Sleep and Circadian Rhythms (2010)
  • Author(s): K.Honma
  • Organizer: 11^<th> Annual Meeting of Sleep Medicine, Ozsetleri
  • Place of Presentation: Antaiya, Turkey
  • Year and Date: 2010-11-09
• [Presentation] Spatiotemporal analysis of Clock gene Per1 and Per2 expression in the rat suprachiasmatic nucleus. (2010)
  • Author(s): N.Tokumaru, S.Honma, K.Honma
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka
  • Year and Date: 2010-05-21
• [Presentation] Perturbation of circadian oscillation in cultured mouse suprachiasmatic nucleus by protein synthesis inhibitor (2010)
  • Author(s): S.Nishide, S.Honma, K.Honma
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka
  • Year and Date: 2010-05-21
• [Presentation] Acceleration of photic entrainment by non-photic time cues in mouse : effects of exercise and/or stress on central and peripheral clocks (2010)
  • Author(s): Y.Yamanaka, S.Honma, K.Honma
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka
  • Year and Date: 2010-05-21
• [Presentation] Rapid shift of social schedule induces an internal desynchronization between sleep-wake cycle and plasma melatonin rhythm. (2009)
  • Author(s): Yamanaka Y.
  • Organizer: The ASRS summit and symposium on sleep research and medicine, Abst., p.31
  • Place of Presentation: Nago (Japan)
  • Year and Date: 2009-10-31
• [Presentation] Two differential molecular mechanisms for circadian oscillation in the suprachiasmatic nucleus. (2009)
  • Author(s): Ono D. Honma S., Honma K.
  • Organizer: ASRS summit and symposium on sleep research and medicine, Abst., p.53
  • Place of Presentation: Nago (Japan)
  • Year and Date: 2009-10-30
• [Presentation] Molecular events associated with resetting the circadian oscillation by protein synthesis inhibitor in mouse suprachiasmatic nucleus. (2009)
  • Author(s): Nishida S., Honma S., Honma K.
  • Organizer: ASRS summit and symposium on sleep research and medicine, Abst., p.54
  • Place of Presentation: Nago (Japan)
  • Year and Date: 2009-10-30
• [Presentation] Seasonality in sleep and wakefulness. (2009)
  • Author(s): Honma S., Yoshikawa T., Hashimoto S, Honma K.
  • Organizer: 6th Congress of Asian Sleep Research Society, Symposium 'Sleep as a Biological Rhythm', Abstract p.99
  • Place of Presentation: Osaka (Japan)
  • Year and Date: 2009-10-26
• [Presentation] Non-photic time cues phase-dependently accelerate phase-shifts of mouse peripheral clocks. (2009)
  • Author(s): Yamanaka Y., Honma S., Honma K.
  • Organizer: XI Congress of the European Biological Rhythms Society, Symposium 'Entrainment by non-photic cues', Abst.P.58
  • Place of Presentation: Strasburg (France)
  • Year and Date: 2009-08-25
• [Presentation] Molecular mechanism of circadian oscillation reset by prolonged treatment of cycloheximide in mouse suprachiasmatic nucleus. (2009)
  • Author(s): Nishide S., Honma S., Yamada Y., Honma K.
  • Organizer: XI Congress of the European Biological Rhythms Society, Poster Session, Abst.P.124
  • Place of Presentation: Strasburg (France)
  • Year and Date: 2009-08-25
• [Presentation] Circadian firing rhythms in Cry1/Cry2 double-deficient mice in the cultured suprachiasmatic nucleus. (2009)
  • Author(s): Ono D., Honma S., Honma K.
  • Organizer: XI Congress of the European Biological Rhythms Society, Symposium 'Exploring spatoptemporal organization of SCN circuits', Abst.P.64
  • Place of Presentation: Strasburg (France)
  • Year and Date: 2009-08-24
• [Presentation] SCN-independent clocks : a possible mechanism of non-photic entrainment in humans. (2009)
  • Author(s): Honma K.
  • Organizer: International Symposium on Biological Rhythm, Abstr..14
  • Place of Presentation: Sapporo (Japan)
  • Year and Date: 2009-08-03
• [Presentation] Molecular mechanisms of terminating and restarting circadian rhythms in the mouse suprachiasmatic nucleus. (2009)
  • Author(s): Nishide S., Honma S., Yamada Y., Hamada T., Honma K.
  • Organizer: 36^<th> International Congress of Physiological Sciences, Poser Session
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2009-08-01
• [Presentation] Effects of ambient temperature during maternal deprivation on circadian rhythms of neonatal rats. (2009)
  • Author(s): Yoshikawa T., Matsuno A., Yamanaka Y., Nishide S., Honma S., Honma K.
  • Organizer: 36^<th> International Congress of Physiological Sciences, Poser Session
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2009-08-01
• [Presentation] Re-entrainment of circadian rhythms after 8 h advanced light-dark cycles depends on the timing of exposure to novel environment in mice. (2009)
  • Author(s): Yamanaka Y., Honma S., Honma K.
  • Organizer: 36^<th> International Congress of Physiological Sciences, Poser Session
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2009-08-01
• [Presentation] Differential responses of the circadian rhythms in mouse suprachiasmatic nucleus cells to protein synthesis inhibitor, cyclohezimide. (2009)
  • Author(s): Ono D., Honma S., Honma K.
  • Organizer: 36^<th> International Congress of Physiological Sciences, Poser Session
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2009-08-01
• [Presentation] Clock mechanisms in mammals encoding photoperiods. (2009)
  • Author(s): Honma S., Yoshikawa T., Honma K.
  • Organizer: 36^<th> International Congress of Physiological Sciences, Whole-day Symposium 'Temporal organization of physiology and behavior'
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2009-07-30
• [Presentation] Circadian organization in humans : is there any oscillator(s) for behavioral rhythms independent of those in the SCN? (2009)
  • Author(s): Honma K.
  • Organizer: 36^<th> International Congress of Physiological Sciences, Whole-day Symposium 'Temporal organization of physiology and behavior'
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2009-07-30
• [Book] Bioluminescent Imaging for Assessing Heterogenous Cell Functions in the Mammalian Central Circadian Clock.((eds) N.Tamaki and Y. Kuge)(Molecular Imaging for Integrated Medical Therapy and Drug Development) (2010)
  • Author(s): Honma S., T.Yoshikawa, S.Nishide, D.Ono, K.Honma
  • Total Pages: 189-196
  • Publisher: Springer, Tokyo
• [Remarks]
  • URL: http://babu.med.hokudai.ac.jp/~phys~1w/
• [Patent(Industrial Property Rights)] ターゲット位置追跡装置およびターゲット発光検出装置 (2010)
  • Inventor(s): 石川正純、浜田俊幸、本間研一、本間さと
  • Industrial Property Rights Holder: 北海道大学
  • Industrial Property Number: 特許,特願2010-245624
  • Filing Date: 2010-11-01