Medical innovation for diabetes care by introducing non-invasive diagnostic system against small tissues

2011 Fiscal Year Final Research Report

• Project/Area Number: 20249057
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Tohoku University
• Principal Investigator: SATOMI Susumu 東北大学, 病院, 教授 (00154120)
• Co-Investigator(Kenkyū-buntansha): MATSUE Tomokazu 東北大学, 原子分子材料科学高等研究機構, 教授 (70173797) ; FUJIMORI Keise 東北大学, 病院, 准教授 (50238622) ; GOTO Masafumi 東北大学, 未来科学技術共同研究センター, 教授 (50400453)
• Project Period (FY): 2008 – 2011
• Keywords: 糖尿病 / 細胞 / 組織 / 膵島 / 移植

Research Abstract

In the present study, clinically applicable new system in which the respiratory activity of multiple small tissues can be measured accurately in 15 minutes was successfully established. By applying this novel system, it was demonstrated that the glucose stimulated respiratory activity of isolated pancreatic islets was significantly correlated with the graft function after islet transplantation, suggesting that this could be used as a reliable potency test for isolated islets. Furthermore, it was also proved that established system in the present study could be useful for developing a new enzyme of islet isolation and a new culture device of isolated islets.

Research Products

• [Journal Article] Assessment for revascularization of transplanted pancreatic islets at subcutaneous site in mice with a highly sensitive imaging system (2011)
  • Author(s): Nishimura R, Goto M, Sekiguchi S, Fujimori K, Ushiyama A, Satomi S.
  • Journal Title: Transplantation Proc Volume: 43 Pages: 3239-3240
  • Peer Reviewed
• [Journal Article] Effect of synthetic protease inhibitor gabexate mesilate on attenuation of coagulant activity and cytokine release in a rat model of islet transplantation (2011)
  • Author(s): Tokodai K, Goto M, Inagaki A, Imura T, Satomi S
  • Journal Title: Transplantation Proc Volume: 43 Pages: 3176-3178
  • Peer Reviewed
• [Journal Article] 吸入麻酔イソフルランが膵島移植に及ぼす影響 (2011)
  • Author(s): 山谷英之、後藤昌史、Feng Zhaoqiang、猪村武弘、小林英司、里見進
  • Journal Title: 移植 Volume: 46 Pages: 154-162
  • Peer Reviewed
• [Journal Article] ここまで進んだ膵島移植 (2010)
  • Author(s): 後藤昌史
  • Journal Title: 糖尿病診療マスター Volume: 8 Pages: 203-207
• [Journal Article] Chronoamperometric characterization of secreted alkaline phosphates from single cell entrapped in a poly(dimethylsiloxisane)micro well (2010)
  • Author(s): H. Shiku, J. Suzuki, T. Murata, K. Ino, T. Matsue
  • Journal Title: Electrochim. Acta Volume: 55 Pages: 8263-8267
  • Peer Reviewed
• [Journal Article] Simultaneous non-contact topography and electrochemical imaging by SECM/SICM featuring ion current feedback regulation (2010)
  • Author(s): Y. Takahashi, A. I. Shevchuk, P. Novak, Y. Murakami, H. Shiku, Y. E. Korchev, T. Matsue
  • Journal Title: J. Am. Chem. Soc Volume: 132 Pages: 10118-10126
  • Peer Reviewed
• [Journal Article] C5a-Inhibitory peptide combined with gabexate mesilate prevents the Instant Blood-Mediated Inflammatory Reaction in a rat model of islet transplan (2010)
  • Author(s): Tokodai K, Goto M, Inagaki A, Nakanishi W, Okada N, Okada H, Satomi S
  • Journal Title: Transplantation Proc Volume: 42 Pages: 2102-2103
  • Peer Reviewed
• [Journal Article] Attenuation of cross-talk between the complement and coagulation cascades by C5a blockade improves early outcomes after intraportal islet transplantation (2010)
  • Author(s): Tokodai K, Goto M, Inagaki A, Nakanishi W, Ogawa N, Satoh K, Kawagishi N, Sekiguchi S, Nilsson B, Okada N, Okada H, Satomi S
  • Journal Title: Transplantation Volume: 90 Pages: 1358-1365
  • Peer Reviewed
• [Journal Article] The impact of ischemic stress on the quality of isolated pancreatic islets (2010)
  • Author(s): Goto M, Imura T, Inagaki A, Ogawa N, Yamaya H, Fujimori K, Kurokawa Y, Satomi S
  • Journal Title: Transplantation Proc Volume: 42 Pages: 2040-2042
  • Peer Reviewed
• [Journal Article] Quantification of the islet product : Presentation of a standardized current Good Manufacturing Practices compliant system with minimal variability (2010)
  • Author(s): Friberg A, Brandhorst H, Buchwald P, Goto M, Ricordi C, Brandhorst D, Korsgren O
  • Journal Title: Transplantation Volume: 91 Pages: 677-683
  • Peer Reviewed
• [Journal Article] A novel predictive method for assesing the quality of isolated pancreatic islets using a scanning electrochemical microscopy (2009)
  • Author(s): Goto M, Abe H, Ito-Sasaki T, Goto M, Inagaki A, Ogawa N, Fujimori K, Kurokawa Y, Matsue T, Satomi S
  • Journal Title: Transplantation Proc Volume: 41(1) Pages: 311-313
  • Peer Reviewed
• [Journal Article] The influence of brain death on tissue factor expression in the pancreatic tissues and isolated islets in rats (2009)
  • Author(s): Saito Y, Goto M, Maya K, Ogawa N, Fujimori K, Kurokawa Y, Satomi S
  • Journal Title: Transplantation Proc Volume: 41(1) Pages: 307-310
  • Peer Reviewed
• [Journal Article] Optimization of a Prominent Oxygen-Permeable Device for Pancreatic Islets (2009)
  • Author(s): Goto M, Yoshikawa Y, Matsuo K, Shirasu A, Ogawa N, Takahashi H, Saitoh Y, Fujimori K, Kurokawa Y, Tamai M, Satomi S
  • Journal Title: Transplantation Proc Volume: 40(2) Pages: 411-412
  • Peer Reviewed
• [Journal Article] 膵島移植の現状と展望 (2009)
  • Author(s): 後藤昌史
  • Journal Title: 最新医学 Volume: 64(2) Pages: 45-52
• [Journal Article] ラット膵島移植モデルにおける移植前培養膵島に対する新鮮膵島の優位性の検証 (2009)
  • Author(s): 高橋英幸、後藤昌史、小川則彦、藤盛啓成、黒川良望、土井秀之、里見進
  • Journal Title: 移植 Volume: 44(1) Pages: 82-90
  • Peer Reviewed
• [Journal Article] Superiority of fresh islets compared with cultured islets (2009)
  • Author(s): Takahashi H, Goto M, Ogawa N, Saito Y, Fujimori K, Kurokawa Y, Doi H, Satomi S
  • Journal Title: Transplantation Proc Volume: 41(1) Pages: 350-351
  • Peer Reviewed
• [Journal Article] Entrapment and Measurement of a Biologically Functionalized Micro bead with a Micro well Electrode. (2009)
  • Author(s): C. Y. Chang, Y. Takahashi, T. Murata, H. Shiku, H. C. Chang, T. Matsue
  • Journal Title: Lab Chip Volume: 9 Pages: 1185-1192
  • Peer Reviewed
• [Presentation] 高度炎症を伴う膵動静脈奇形に対する自家膵島移植3例の検証 (2012)
  • Author(s): 後藤昌史
  • Organizer: 第39回日本膵膵島移植研究会
  • Place of Presentation: 旭川
  • Year and Date: 2012-03-10
• [Presentation] 糖尿病治療のメディカルイノベーション (2011)
  • Author(s): 後藤昌史
  • Organizer: 第47回日本移植学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-05
• [Presentation] 臨床応用を目的とした大動物モデルにおける新規膵島評価法の有用性に関する検証 (2011)
  • Author(s): 後藤昌史
  • Organizer: 第47回日本移植学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-05
• [Presentation] A novel potency test for assessing the quality of isolated pancreatic islets using a scanning electrochemical microscopy (2011)
  • Author(s): Masafumi Goto
  • Organizer: International Pancreas and Islet Transplant Association
  • Place of Presentation: プラハ(チェコスロバキア)
  • Year and Date: 2011-06-02
• [Presentation] Development of a highly efficient collagenase for isolating pancreatic islets (2011)
  • Author(s): Masafumi Goto
  • Organizer: International Pancreas and Islet Transplant Association (IPITA)
  • Place of Presentation: プラハ(チェコスロバキア)
  • Year and Date: 2011-06-02
• [Presentation] Successful islet autotransplantation under severe inflammatory status due to a rupture of the pancreatic arteriovenous malformation by introducing a novel graft protecting protocol (2011)
  • Author(s): Masafumi Goto
  • Organizer: International Pancreas and Islet Transplant Association (IPITA)
  • Place of Presentation: プラハ(チェコスロバキア)
  • Year and Date: 2011-06-02
• [Presentation] 膵島移植の現状と今後の展望(依頼講演) (2010)
  • Author(s): 後藤昌史
  • Organizer: 第13回近畿膵移植検討会
  • Place of Presentation: 大阪
  • Year and Date: 2010-12-04
• [Presentation] 現状における臨床グレードの膵島分離用酵素剤の検証 (2010)
  • Author(s): 後藤昌史
  • Organizer: 第46回日本移植学会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Presentation] No Need of Tryptic-like Activity for Successful Porcine Islet Isolation (2010)
  • Author(s): Masafumi Goto
  • Organizer: XXIII International Congress Of The Transplantation Society
  • Place of Presentation: Vancouver (Canada)
  • Year and Date: 2010-08-16
• [Presentation] 膵島分離用酵素剤の活性評価システムの構築 (2010)
  • Author(s): 後藤昌史
  • Organizer: 第37回膵膵島移植研究会
  • Place of Presentation: 宇都宮
  • Year and Date: 2010-03-12
• [Presentation] 膵島移植の現状と今後の展望 (2009)
  • Author(s): 後藤昌史
  • Organizer: 第8回日本再生医療学会
  • Place of Presentation: 東京
  • Year and Date: 20090305-06
• [Presentation] 今後の膵島移植発展への試み (2009)
  • Author(s): 後藤昌史
  • Organizer: 第36回膵膵島移植研究会
  • Year and Date: 20090227-28
• [Presentation] The impact of ischemic stress on the quality of isolated pancreatic islets (2009)
  • Author(s): Masafumi Goto
  • Organizer: 2009 International Pancreas and Islet Transplant Association
  • Place of Presentation: Venice (Italy)
  • Year and Date: 2009-10-13
• [Presentation] Potency tests for assessing the quality of isolated pancreatic islets (2009)
  • Author(s): Masafumi Goto
  • Organizer: The 10th Cell Transplant Society Congress
  • Place of Presentation: Okayama
  • Year and Date: 2009-04-20
• [Presentation] Improvement of preservation and evaluation for pancreatic islets by introducing bioengineering approach (2008)
  • Author(s): Masafumi Goto
  • Organizer: 第35回日本低温医学会総会
  • Place of Presentation: Tokyo
  • Year and Date: 20081122-23
• [Presentation] 東北大学における膵島移植の取り組み (2008)
  • Author(s): 後藤昌史
  • Organizer: 第44回日本移植学会
  • Place of Presentation: 大阪
  • Year and Date: 20080919-21
• [Presentation] 有用な膵島評価法である呼吸活性計測法の実際 (2008)
  • Author(s): 後藤昌史
  • Organizer: 第44回日本移植学会
  • Place of Presentation: 大阪
  • Year and Date: 20080919-21
• [Presentation] Predictive methods for assessing the quality of isolated pancreatic islets (2008)
  • Author(s): Masafumi Goto
  • Organizer: XXII International Congress Of The Transplantation Societ
  • Place of Presentation: Sydney
  • Year and Date: 20080810-14
• [Presentation] A NOVEL PREDICTIVE METHOD FOR ASSESING THE QUALITY OF ISOLATED PANCREATIC ISLETS USING A SCANNING ELECTROCHEMICAL MICROSCOPY (2008)
  • Author(s): Masafumi Goto
  • Organizer: XXII International Congress Of The Transplantation Society
  • Place of Presentation: Sydney
  • Year and Date: 20080810-14