Studies of formation and function of neuronal circuits using alpha-chimerin mutant mice

2010 Fiscal Year Final Research Report

• Project/Area Number: 20300118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute of Genetics
• Principal Investigator: IWASATO Takuji 国立遺伝学研究所, 個体遺伝研究系, 教授 (00311332)
• Co-Investigator(Renkei-kenkyūsha): ITOHARA Shigeyoshi 独立行政法人理化学研究所, 行動遺伝学技術開発チーム, シニアチームリーダー (60252524) ; KATOH Hironori 京都大学, 生命科学研究科, 准教授 (50303847) ; NISHIMARU Hiroshi 筑波大学, 大学院・人間総合科学研究科, 准教授 (20302408)
• Project Period (FY): 2008 – 2010
• Keywords: 神経科学 / マウス / 遺伝学 / 神経回路 / 発達

Research Abstract

We aim to reveal roles of alpha-chimerin Rac-GTPase activating protein(GAP) in formation and function of neuronal circuit in the mammalian brain. We generated global and Cre-mediated conditional knockout mice of alpha-chimerin. Furthermore, we generated knockout mouse lines specific to alpha1 and alpha2 isoforms. By analyzing these mice behaviorally and histologically, we found important roles of alpha-chimerin in hippocampus function.

Research Products

• [Journal Article] Role of adenylate cyclase 1 in retinofugal map development (2011)
  • Author(s): Dande, OS., Bhatt, S., Anishchenko, A, Elstrott, J., Iwasato, T., Swindell, EC, Xu, HP., Jamrich, M., Itohara, S., Feller, MB, Crair, MC.
  • Journal Title: J. Comp. Neurol
  • DOI: doi:10.1002/cne.23000
  • Peer Reviewed
• [Journal Article] Molecular characterization and gene disruption of a novel zinc-finger protein, HIT-4, expressed in rodent brain (2010)
  • Author(s): Tanabe, Y., Hirano, A., Iwasato, T., Itohara, S., et al.
  • Journal Title: J. Neurochem Volume: 112巻 Pages: 1035-1044
  • Peer Reviewed
• [Journal Article] 中枢神経回路の活動依存的精緻化-マウス体性感覚野に「バレル」が形成される仕組み (2009)
  • Author(s): 岩里琢治
  • Journal Title: 生物の科学「遺伝」 Volume: 63巻 Pages: 79-85
• [Journal Article] Altered mnemonic functions and resistance to N-METHYL-d-Aspartate receptor antagonism by forebrain conditional knockout of glycine transporter (2009)
  • Author(s): Singer, P., Yee, BK., Feldon, J., Iwasato, T., Itohara, S., et al.
  • Journal Title: Neuroscience Volume: 161巻 Pages: 635-654
  • Peer Reviewed
• [Journal Article] Beta2-chimaerin binds to EphA receptors and regulates cell migration (2009)
  • Author(s): Takeuchi, S., Yamaki, N., Iwasato, T., Negishi, M., Katoh, H.
  • Journal Title: FEBS Lett Volume: 583巻 Pages: 1237-1242
  • Peer Reviewed
• [Journal Article] Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layerIVbarrel development (2008)
  • Author(s): Iwasato, T., Inan, M., Kanki, H., Erzurumlu, RS., Itohara, S., et al.
  • Journal Title: J. Neurosci Volume: 28巻 Pages: 5931-5943
  • Peer Reviewed
• [Journal Article] BMP signaling through BMPRIA in astrocytes is essential for proper cerebral angiogenesis and formation of the blood-brain-barrier (2008)
  • Author(s): Araya, R., Kubo, M., Ishii, K., Hashikawa, T., Iwasato, T., Itohara, S., Terasaki, T., Oohira, A., Mishina, Y., Yamada, M.
  • Journal Title: Mol. Cell Neurosci Volume: 38巻 Pages: 417-430
  • Peer Reviewed
• [Presentation] Loss of Rac-GAP a-chimerin leads to alterations of emotional and cognitive behaviors in mice (2011)
  • Author(s): Iwata, R., Goto, H., Tanaka, M., Itohara, S., Iwasato, T.
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington, DC
  • Year and Date: 20111112-26
• [Presentation] Loss of Rac-GAP a-chimerin leads to alterations of emotional and cognitive behaviors in mice (2011)
  • Author(s): Iwata, R., Goto, H., Tanaka, M., Itohara, S., Iwasato, T.
  • Organizer: Molecular and cellular cognition society
  • Place of Presentation: Washington, DC
  • Year and Date: 20111110-11
• [Presentation] Rac GAP a-キメリン欠損マウスの網羅的行動解析Comprehensive behavioral analysis of Rac-GAP a-chimaerin deficient mice (2011)
  • Author(s): 岩田亮平.後藤大道.田中三佳.糸原重美
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 20110914-17
• [Presentation] Circuit refinement during postnatal development of the mouse somatosensory cortex (2010)
  • Author(s): 岩里琢治
  • Organizer: BMB2010第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] Comprehensive behavioral analysis of miffy mice, a spontaneous mutant of Rac-GAPα-chimerin (2010)
  • Author(s): Iwata, R., Goto, H., Tanaka, M., Itohara, S., Iwasato, T.
  • Organizer: Molecular and Cellular Cognition Society-Society for Neuroscience Satellite Symposium 2010
  • Place of Presentation: San Diego Convention Center(アメリカ合衆国サンディエゴ)
  • Year and Date: 2010-11-11
• [Presentation] 左右の手足の非対象な動きを可能にする神経回路形成の分子機構 (2008)
  • Author(s): 岩里琢治
  • Organizer: BMB2008第31回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-12
• [Presentation] Dissociation of pre-and postsynaptic mechanisms in maturation of the barrel cortex (2008)
  • Author(s): Iwasato, T.
  • Organizer: The 6th FENS forum of European Neuroscience
  • Place of Presentation: Geneva
  • Year and Date: 2008-07-15
• [Presentation] Dissociation of pre-and postsynaptic mechanisms of barrel cortex development (2008)
  • Author(s): 岩里琢治
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Book] Miffy変異マウス:ウサギのように両足をそろえて歩く自然発生変異マウス(分担執筆)<seriesモデル動物利用マニュアル>疾患モデルの作製と利用-脳・神経疾患(三品昌美編) (2011)
  • Author(s): 岩里琢治,糸原重美
  • Total Pages: 99-110
• [Remarks]
  • URL: http://www.nig.ac.jp/section/iwasato/iwasato-j.html
• [Remarks]
  • URL: http://homepage3.nifty.com/iwasato/