2011 Fiscal Year Final Research Report
Basic research of high-throughput-technology which adds a small amount of liquid reagents using impact force generated with femtosecond laser
| Project/Area Number |
20310075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Tohoku Fukushi University (2010-2011)
Nara Institute of Science and Technology (2008-2009) |
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Principal Investigator |
OKANO Kazunori 東北福祉大学, 感性福祉研究所, 研究員 (00443250)
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| Co-Investigator(Kenkyū-buntansha) |
MASUHARA Hiroshi 奈良先端科学技術大学院大学, 特任教授 (60029551)
HOSOKAWA Yoichiroh 奈良先端科学技術大学院大学, 特任准教授 (20448088)
UWADA Takayuki 奈良先端科学技術大学院大学, 研究員 (30455448)
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| Project Period (FY) |
2008 – 2011
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| Keywords | マイクロバイオシステム / フェムト秒レーザー / 極微量液中試薬添加 / レーザー誘起応力波 / 細胞刺激 / 細胞アレイ / 液中レーザーリソグラフィー |
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| Research Abstract |
We developed fundamental technologies by using a femtosecond laser(fs laser) on living-cell stimulation and manipulation. 1) Drags were delivered to limited area in culture medium by applying an fs laser-induced impact for bombing targeted cells with the drags. 2) The impact could release a targeted cell from a culture plate and delivered to a desired position. 3) Surface characteristic of the culture platform was changed from cytophobic to cytophilic when the fs laser was focused to the platform coated with cytophobic polymer. In conclusion, this fs-laser process in aqueous solution achieved the limited cell stimulation and the multiple cell arrangement.
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[Remarks] (1) K. Okano, Induction of Cell-Cell Connections by Using in situ Laser Lithography and Monitoring of Cell Activities on a Solid Platform, The 3rd Workshop on Laser Bio/Nano Sci., March 4, 2011, National Chiao-Tung Univ.(Hsinchu, Taiwan)(Invit ed)
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[Remarks] (2)坪川宏,岡野和宣,細胞間ネットワーク構築の時空間的制御が可能なプラットホームの開発,電気通信研究所共同プロジェクト研究会「ナノ・バイオの融合による新規バイオデバイスに関する研究」第16回情報バイオトロニクス研究会, 2011.2.3,電気通信研究所(仙台) Invited)
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[Remarks] (3) K. Okano, Induction of the intercellular connections by using in-situ laser lithography and monitoring of cells responding to drags on a solid platform, The 2nd Workshop on Laser Bio/Nano Sci., Sept. 6, 2010, Nara Inst. Sci. Technol.(Ikoma, Japan)(Invited)
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[Remarks] (4) K, Okano, D. Yu, I. Liau, Cell arrangement and connection of individual cells on a cell array platform by femtosecond laser, The 1st NCTU-NAIST Workshop on "Molecular/Nano Sci." Nov. 11-13, 2009, National Chiao-Tung Univ.(Hsinchu, Taiwan)(Invited).
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[Remarks] (5) Y. Yasuyo, Y. Hosokawa, K. Okano, M. Matsubara, H. Masuhara, Fluorescence visualization of cell detachment process from a substrate by femtosecond laser "tsunami", The 1st NCTU-NAIST Workshop on "Molecular/Nano Sci.", Nov. 11-13, 2009, National Chiao-Tung Univ.(Hsinchu, Taiwan)
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[Remarks] (6) A. Matsui, Y. Maezawa, Y. Hosokawa, K. Okano, M. Matsui, H. Masuhara, Fabrication of cell-array platform by femtosecond laser process in culture medium, The 1st NCTU-NAIST Workshop on "Molecular/Nano Sci." Nov. 11-12, 2009, National Chiao-Tung Univ.(Hsinchu, Taiwan)
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[Remarks] (7) Y. Maezawa, Y. Hosokawa, K. Okano, M. Matsubara, H. Masuhara, Individual sorting of animal cells by femtosecond laser induced-stress wave, The 8th Gist/NIST joint symposium on advanced materials, Nov. 26-27, 2008, Nara Inst. Sci. Technol.(Ikoma, Japan
