2010 Fiscal Year Final Research Report
Genome-wide analysis and its application in Chinese hamster ovary cells
| Project/Area Number |
20360373
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
OMASA Takeshi Osaka University, 大学院・ソシオテクノサイエンス研究部, 教授 (00252586)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIRAI Akihiro 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (40380117)
OHTAKE Hisao 大阪大学, 大学院・工学研究科, 教授 (10127483)
HONDA Kohsuke 大阪大学, 大学院・工学研究科, 准教授 (90403162)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | CHO細胞 / ゲノム / BACライブラリー / 染色体 |
|---|
| Research Abstract |
Chinese hamster ovary (CHO) cells are the most dependable host cells for the industrial production of therapeutic proteins. We constructed CHO genomic bacterial artificial chromosome (BAC) library from a gene-amplified CHO cell line for genome-wide analysis of CHO cell lines. CHO chromosomal markers were constructed by fluorescence in situ hybridization (FISH) imaging using BAC clones as hybridization probes (BAC-FISH). Using constructed tools, we could analyze chromosome rearrangement in CHO cell line.
|
Research Products
(16 results)
-
[Journal Article] Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells2010
Author(s)
Wook-Dong Kim, Miwako Tokunaga, Hiroyuki Ozaki, Takuya Ishibashi, Kohsuke Honda, Hiroyuki Kajiura, Kazuhito Fujiyama, Ryutaro Asano, Tzumi Kumagai, Takeshi Omasa, Hisao Ohtake
-
Journal Title
Applied Microbiology and Biotechnology Volume 85
Pages: 535-542
Peer Reviewed
-
-
-
-
-
[Journal Article] Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells2009
Author(s)
Takeshi Omasa, Yihua Cao,, Joon Young Park, Yasuhiro Takagi, Shuichi Kimura, Hidenori Yano, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Hisao Ohtake
-
Journal Title
Biotechnology and Bioengineering Volume 104, No.5
Pages: 986-994
Peer Reviewed
-
-
-
-
-
-
-
[Presentation] Chinese Hamster Ovary Cells BAC library : Application to the characterization of CRC chromosomes2008
Author(s)
Takeshi Omasa, Yihua Cao, Shuiichi Kimura, S.M.A. Haghparast, Yasuhiro Takagi, Kohsuke Ronda, Hisao Ohtake
Organizer
In ; The thirteenth international biotechnology symposium and exhibition (IBS2008), IL-116, Journal of Biotechnology, vol.136S, p. S141
Place of Presentation
Dalian, China
Year and Date
2008-10-16
-
[Presentation] Analysis of the specific chromosomal region adjacent to the DHFR gene amplied in CHO DR 1000L-4N cell line2008
Author(s)
Takeshi Omasa, Yihua Cao, Joon-Young Park, Yasuhiro Takagi, Hidenori Yano, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Hisao Ohtake
Organizer
In ; Engineering Conference International : Cell Culture Engineering XI, 1.B.11, p.105
Place of Presentation
Sunshine Coast, Quennsland, Australia
Year and Date
2008-04-14
-
-
