2010 Fiscal Year Final Research Report
Development of fundamental technology for accurate RNAomics
| Project/Area Number |
20360374
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
FUKUSAKI Eiichiro Osaka University, 工学研究科, 教授 (40273594)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAMURA Yuzuru 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 准教授 (20290877)
KUWAHARA Masayasu 群馬大学, 工学研究科, 准教授 (40334130)
OKAZAWA Atsushi 大阪大学, 工学研究科, 助教 (10294042)
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| Project Period (FY) |
2008 – 2010
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| Keywords | メタボロミクス / LC / MS / RNA / miRNA |
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| Research Abstract |
Fundamental technology including separation and mass spectrometry for miRNA measurement have been studied. Micro flow meter based separation system was optimized. LC system was established through optimization of elutant, matrix, column etc. De novo sequencing of miRNA (longer than 20 base) was achieved by means of iontrap-time of flight mass spectrometry based MS(N) analysis. Ion mobility based mass spectrometry was also performed to separate sequence isomers. In addition, RNA analogous MS probe was created.
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[Journal Article] Simultaneous quantification of lignans in Arabidopsisthaliana by highly sensitive capillary liquidchromatography-electrospray ionization-ion trapmass spectrometry2011
Author(s)
Okazawa, A., Hori, K., Okumura, R., Izumi, Y., Hata, N., Bamba, T., Fukusaki E., Ono, E., Satake, H., Kobayashi, A
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Journal Title
Plant Biotechnol. (in press)
Peer Reviewed
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[Journal Article] Metabolomics-based systematic predictionof yeast lifespan and its application for semirationalscreening of aging-related mutants2010
Author(s)
Yoshida, R., T, Tamura, C., Takaoka, K., Harada, A., Kobayashi, Y., Mukai, E., E.Fukusaki
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Journal Title
Aging Cell 9(4)
Pages: 616-625
Peer Reviewed
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[Journal Article] Influences of biofluid sample collection and handling procedureson GC-MS based metabolomic studies2010
Author(s)
Bando, K., R., Kawahara, T., Kunimatsu, J., Sakai, J., Kimura, H., Funabashi, T., Seki, T., Bamba, E., E.Fukusaki
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Journal Title
J Biosci Bioeng 110(4)
Pages: 491-499
Peer Reviewed
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[Journal Article] GC-MS-based metabolomics reveals mechanism of action for hydrazine induced hepatotoxicity in rats2010
Author(s)
Bando, K., T., Kunimatsu, J., Sakai, J., Kimura, H., Funabashi, T., Seki, T., Bamba, E., E.Fukusaki
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Journal Title
Peer Reviewed
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[Journal Article] Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves2009
Author(s)
Jumtee, K., Okazawa, A., Harada, K., Fukusaki E., Takano, M., Kobayashi, A
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Journal Title
J. Biosci.Bioeng 108
Pages: 151-159
Peer Reviewed
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[Journal Article] Unique phytochrome responses of the holoparasitic plant2009
Author(s)
Takagi, K., Okazawa, A., Wada, Y., Mongkolchaiphruek, A., Fukusaki E., Yoneyama, K., Takeuchi, Y., Kobayashi, A
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Journal Title
Orobanche minor, New Phytol 182
Pages: 965-974
Peer Reviewed
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[Journal Article] Transcription and reverse transcription of artificial nucleic acids involving backbone modification bytemplate-directed DNA polymerase reactions2009
Author(s)
Kuwahara, H., Takeshima, J., Nagashima,S., Minezaki, H., Ozaki, H., Sawai
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Journal Title
Bioorg. Med. Chem 17
Pages: 3772-3788
Peer Reviewed
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[Journal Article] High-resolution spatial and temporal analysis of phytoalexin production in oats2009
Author(s)
Izumi, Y., Kajiyama, S., Nakamura, R., Ishihara, A., Okazawa, A., Fukusaki E., Kanematsu, Y., Kobayashi, A.
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Journal Title
Planta 229(4)
Pages: 931-934
Peer Reviewed
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[Presentation]2010
Author(s)
Yuuya Kasahara, Yuuta Irisawa, Hidetaka Okonogi, Masayoshi Tajima, Atsushi Kobayashi, Masayasu Kuwahara, Hiroaki Ozaki, Seiji Tobita
Organizer
PACIFICHEM 2010
Place of Presentation
Hawaii Convention Center
Year and Date
2010-12-19
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