2010 Fiscal Year Final Research Report
Study on the common ancestors of Archaea, Bacteria and Commonote based on the resurrected enzymes.
| Project/Area Number |
20370094
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
YAMAGISHI Akihiko Tokyo University of Pharmacy and Life Science, 生命科学部, 教授 (50158086)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOBORI Shin-Ichi 東京薬科大学, 生命科学部, 講師 (40291702)
AKANUMA Satoshi 東京薬科大学, 生命科学部, 助教 (10321720)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 機能進化 |
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| Research Abstract |
Ancestral enzymes of translation system (GlyRS), metabolic system (IPMDH and NDK) and replication system (gyrase) were resurrected, their genes were constructed and expressed in E. coli. The purified enzymes of translation and replication systems showed higher thermal stability than counter parts of an extreme thermophile. Several mutants of previously constructed ancestral NDH were analyzed. The results showed that ancestral enzymes of Bacteria, Archaea and Commonote are extremely (>100℃) thermostable.
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