2010 Fiscal Year Final Research Report
Development of a new virus-induced gene silencing vector using Apple latent spherical virus
| Project/Area Number |
20380025
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Iwate University |
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Principal Investigator |
YOSHIKAWA Nobuyuki Iwate University, 農学部, 教授 (40191556)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ウイルスベクター / リンゴ小球形潜在ウイルス(ALSV) / ウイルス誘導ジーンサイレンシング(VIGS) / 遺伝子機能解析 / ダイズ / バラ科果樹 |
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| Research Abstract |
A virus-induced gene silencing (VIGS) system using Apple latent spherical virus (ALSV) vectors was established in legume, cucurbit, Solanaceae species, and Rosaceae fruit trees. ALSV vectors could be successfully used to silence disease resistant N gene in tobacco and RCY1 gene in Arabidopsis thaliana. Infection with an ALSV vector having a fragment of soybean isoflavone synthase 2 gene also led to a reduction of the levels of an isoflavone content in the cotyledons of infected seeds.
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[Journal Article] Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes2009
Author(s)
Igarashi A., Yamagata K., Sugai T.,Takahashi Y., Sugawara E., Tamura A., Uaegasjo H., Yamagishi, N., Takahashi T., Isogai M., Takahashi H., Yoshikawa N.
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Journal Title
Virology 386
Pages: 407-416
Peer Reviewed
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