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2010 Fiscal Year Final Research Report

Analysis of structural elements controlling transglycosylation and its application

Research Project

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Project/Area Number 20380056
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionHokkaido University

Principal Investigator

KIMURA Atsuo  Hokkaido University, 大学院・農学研究院, 教授 (90186312)

Co-Investigator(Kenkyū-buntansha) OKUYAMA Masayuki  北海道大学, 大学院・農学研究院, 助教 (00344490)
Project Period (FY) 2008 – 2010
Keywords糖転移 / 構造因子 / 分子解析
Research Abstract

Glycosylases are enzymes, which are currently utilized for the production of oligosaccharides. Interestingly, production-ability is dependent on the kinds of glycosylases, meaning that their structural elements contribute to the reaction specificity. Our research aims at elucidating structural elements of glycosylase-catalyzed transglycosylation. Our research achieved i) elucidation of structural elements contributing to the oligosaccharide production ; ii) establishment of the theoretical background of oligosaccharide production ; iii) finding the ways to regulate the oligosaccharide production.

  • Research Products

    (28 results)

All 2011 2010 2009 2008 Other

All Journal Article (17 results) Presentation (9 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Bioengineered glucansucrase of Leuconostoc mesenteroides for preferred oligosaccharide synthesis using sucrose2011

    • Author(s)
      Kang HK, Kimura A, Kim D
    • Journal Title

      J Agric Food Chem (in press)

      Pages: IF=2.469

  • [Journal Article] Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity2011

    • Author(s)
      Kim YM, Shimizu R, Nakai H, Mori H, Okuyama M, Kang MS, Fujimoto Z, Funane K, Kim D, Kimura A
    • Journal Title

      Appl Microbiol Biotechnol (in press)

      Pages: IF=2.896

  • [Journal Article] Function of C-terminal region of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulams T-30402011

    • Author(s)
      Funane K, Kawabata Y, Kim YM, Kang HK, Fujimoto Z, Kimura A, Kobayashi M
    • Journal Title

      Biochim Biophys Acta 1814

      Pages: 428-434, IF=2.480

  • [Journal Article] Suicide substrate-based inactivation of endodextranase by 1-epoxyalkyl <-D-glucopyranosides.2010

    • Author(s)
      Kang HK, Kim YM, Nakai H, Kang MS, Hakamada W, Okuyama M, Mori H, Nishio T, Kimura A
    • Journal Title

      J Appl Glycosci 57

      Pages: 111-117

  • [Journal Article] Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities2010

    • Author(s)
      Ryu HJ, Jin X, Lee JH, Woo HJ, Kim YM, Kim GJ, Seo ES, Kang HK, Kim J, Cho DL, Kimura A, Kim D
    • Journal Title

      Enzyme Microb Technol 47

      Pages: 212-215, IF=2.638

  • [Journal Article] Ketudat-Cairns JR : Rice Os4BGlu12 is a wound-induced [○!R]-glucosidase that hydrolyzes cell-wall-[○!R]-glucan-derived oligosaccharides and glycosides.2010

    • Author(s)
      Opassiri R, Maneesan J, Akiyama T, Pomthong B, Jin S, Kimura A
    • Journal Title

      Plant Sci 179

      Pages: 273-280, IF=2.050

  • [Journal Article] Catalytic reaction mechanism based on α-secondary deuterium isotope effects in hydrolysis of trehalose by European honeybee trehalase.2009

    • Author(s)
      Mori H, Lee JH, Okuyama M, Nishimoto M, Oguchi M, Kim D, Kimura A, Chiba S
    • Journal Title

      Biosci Biotechnol Biochem 73

      Pages: 2466-2473, IF=1.326

  • [Journal Article] The first α-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 31.2009

    • Author(s)
      Kang MS, Okuyama M, Mori H, Kimura A
    • Journal Title

      Biochimie 91

      Pages: 1434-1442, IF=3.897

  • [Journal Article] Catalytic mechanism of retaining α-galactosidase belonging to glycoside hydrolase family 97.2009

    • Author(s)
      Okuyama M, Kitamura M, Hondoh H, Kang MS, Mori H, Kimura A, Tanaka I, Yao M
    • Journal Title

      J Mol Biol 392

      Pages: 1232-1241, IF=3.871

  • [Journal Article] Structural comparison of Streptococcus mutans dextran glucosidase with glucoside hydrolases in GH13.2009

    • Author(s)
      Hondoh H, Otsuka-Rachi H, Saburi W, Mori H, Okuyama M, Kimura A
    • Journal Title

      J Appl Glycosci 56(2)

      Pages: 111-117

  • [Journal Article] Construction of a fusion enzyme of dextransucrase and dextranase : Application for one-step synthesis of isomaltooligosaccharides.2009

    • Author(s)
      Kim YM, Seo MY, Kang HK, Kimura A, Kim D
    • Journal Title

      Enzyme Microb Technol 44

      Pages: 159-164, IF=2.638

  • [Journal Article] Structural and functional analysis of a glycoside hydrolase family 97 enzyme from Bacteroides thetaiotaomicron2008

    • Author(s)
      Kitamura M, Okuyama M, Tanzawa F, Mori H, Kitago Y, Watanabe N, Kimura A, Tanaka I, Yao M
    • Journal Title

      J Biol Chem 283

      Pages: 36328-36337, IF=5.520

  • [Journal Article] Isolation of Bacillus and Paenibacillus bacterial strains that produce large molecular of cyclic isomaltooligosaccharides.2008

    • Author(s)
      Funane K, Terasawa K, Mizuno Y, Ono H, Gibu S, Tokashiki T, Kawabata Y, Kim YM, Kimura A, Kobayashi M
    • Journal Title

      Biosci Biotechnol Biochem 72

      Pages: 3277-3280, IF=1.390

  • [Journal Article] Enzymatic synthesis of α-2-deoxyglucosyl derivatives catalyzed by organic solvent-resistant α-glucosidase.2008

    • Author(s)
      Kim YM, Kim D, Kimura A
    • Journal Title

      Biotechnol Bioprocess Eng 13

      Pages: 639-645, IF=1.653

  • [Journal Article] Structure-function relationship of substrate length specificity of dextran glucosidase from Streptococcus mutans.2008

    • Author(s)
      Saburi W, Hondoh H, Kim YM, Mori H, Okuyama M, Kimura A
    • Journal Title

      Biologia 63

      Pages: 1000-1005, IF=0.406

  • [Journal Article] Substrate recognition of Escherichia coli YicI (α-xylosidase)2008

    • Author(s)
      Okuyama M, Kang MS, Yaoi K, Mitsuishi Y, Mori H, Kimura A
    • Journal Title

      J Appl Glycosci 55

      Pages: 111-118

  • [Journal Article] Substrate recognition of α-1,6-glucosidic linkage hydrolyzing enzyme, dextran glucosidase from Streptococcus mutans2008

    • Author(s)
      Hondoh H, Saburi W, Mori H, Okuyama M, Nakada T, Matsuura Y, Kimura A
    • Journal Title

      J Mol Biol 378

      Pages: 911-920, IF=4.146

  • [Presentation] Structural element determining the diverse specificities for chain-length of substrate in glycoside hydrolase family 31 α-glucosidases.2011

    • Author(s)
      Tagami T, Okuyama M, Mori H, Kimura A
    • Organizer
      9th Carbohydrate Bioengineering Meeting.
    • Place of Presentation
      Calouste Gulbenkian Foundation (Lisbon, Portugal)(ポスター賞1位を受賞)
    • Year and Date
      20110515-20110518
  • [Presentation] Molecular Mechanism of novel enzymes degrading and forming α-1,6-glucosidic linkages, including megalosaccharide production.2011

    • Author(s)
      Kim YM, Kanegae M, Hondoh H, Nishimura T, Saburi W, Sadahiro J, Lang W, Shimizu R, Nakai H, Kang HK, Okuyama M, Mori H, Kang MS, Fujimoto Z, Funane K, Kim D, Kimura A
    • Organizer
      The 78th Annual Meeting and International Symposium in 2011 Annual Conference of Korean Society of Food Science and Technology.
    • Place of Presentation
      Daegu Exhibition Convention Center (Daegu, Korea).
    • Year and Date
      2011-06-09
  • [Presentation] Putative role of Tyr227, Gln349 and Leu350 near catalytic amino acids of honeybee α-glucosidase III2010

    • Author(s)
      Ngiwsara L, Iwai G, Mori H, Okuyama M, Kimura A
    • Organizer
      4th Symposium on the Alpha-Amylase Family
    • Place of Presentation
      Smolenice Cattle (Smolenice, Slovakia)(ポスター賞3位を受賞)
    • Year and Date
      20100925-20100930
  • [Presentation] Shifting specificity of Aspergillus niger α-glucosidase from short chain substrates to long chain substrates2010

    • Author(s)
      Tagami T, Nishimura T, Okuyama M, Mori H, Kimura A
    • Organizer
      Plant Polysaccharide and Applied Glycoscience Workshop 2010
    • Place of Presentation
      Sasagawa Hall (Tokyo, Japan).
    • Year and Date
      20100729-20100731
  • [Presentation] 糖質酵素の基礎研究とその展開2010

    • Author(s)
      木村淳夫
    • Organizer
      日本応用糖質科学会平成22年度大会(第59回)
    • Place of Presentation
      グランシップ(静岡県)
    • Year and Date
      2010-09-17
  • [Presentation] α-グリコシダーゼの機能と構造に関する研究2010

    • Author(s)
      奥山正幸
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都)(日本農芸化学会奨励賞受賞講演)
    • Year and Date
      2010-03-28
  • [Presentation] Transglucosylation pattern of wild-type and specificity-altered dextran glucosidase2009

    • Author(s)
      Hondoh H, Otsuka-Rachi H, Saburi W, Mori H, Okuyama M, Kimura A
    • Organizer
      15th European Carbohydrate Symposium
    • Place of Presentation
      University of Vienna (Vienna, Austria)
    • Year and Date
      20090719-20090724
  • [Presentation] Novel cycloisomaltooligosaccharides of large molecule found in culture supernatant of Bacillus and Paenibacillus strains2008

    • Author(s)
      Funane K, Ono H, Gibu S, Tokashiki T, Kawabata Y, Kim Y-M, Kimura A, Kobayashi M
    • Organizer
      24th International Carbohydrate Symposium
    • Place of Presentation
      University of Oslo (Oslo, Norway)
    • Year and Date
      20080727-20080801
  • [Presentation] Identification of essential catalytic residues in an Escherichia coli trehalase and an inverting glycosynthase reaction catalyzed by the catalytic basemutated enzyme2008

    • Author(s)
      Mori H, Saito S, Ogawa K, Maki M, Okuyama M, Kimura A
    • Organizer
      24th International Carbohydrate Symposium(July 27th to August 1st)
    • Place of Presentation
      University of Oslo (Oslo, Norway)
    • Year and Date
      20080727-20080801
  • [Book] Carbohydrate-active enzymes : structure, function, and applications(ed.by Park K-H)(Molecular mechanism of α-glucosidase.)2008

    • Author(s)
      Okuyama M, Mori H, Hondoh H, Nakai H, Saburi W, Kang MS, Kim YM, Nishimoto M, Wongchawalit J, Yamamoto T, Son M, Lee JH, Mar SS, Fukuda K, Chiba S, Kimura A
    • Total Pages
      64-76
    • Publisher
      Woodhead Publishing Ltd. (Cambridge, UK)
  • [Remarks] (1)アウトリーチ活動情報 (1)奥山正幸、木村淳夫:独立行政法人科学技術振興機構・平成22年度サイエンス・パートナーシップ・プロジェクト(SPP)事業(北海道札幌藻岩高校) (2)奥山正幸、木村淳夫:独立行政法人科学技術振興機構・平成21年度サイエンス・パートナーシップ・プロジェクト(SPP)事業(北海道札幌藻岩高校) (3)奥山正幸、木村淳夫:独立行政法人科学技術振興機構・平成20年度サイエンス・パートナーシップ・プロジェクト(SPP)事業(北海道札幌藻岩高校) (2)ホームページ等

    • URL

      http://hecate.general.hokudai.ac.jp/welcome/toppage-jpn.html

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Published: 2012-01-26   Modified: 2016-04-21  

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