2010 Fiscal Year Final Research Report
Development of sensory genomics for evaluation of the molecular basis of taste response and palatability distinction
| Project/Area Number |
20380183
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ABE Keiko The University of Tokyo, 大学院・農学生命科学研究科, 特任教授 (10151094)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Shinji 東京大学, 大学院・農学生命科学研究科, 助教 (50376563)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 味覚システム / トランスジェニック動物 / 味覚応答 / 味の嗜好性 / 味覚伝達路 / 味細胞系譜 |
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| Research Abstract |
Using mice with a WGA tracer introduced, we analyzed the genes expressions in taste cells, taste neurons and cerebral cells by transcriptome, and revealed the molecular properties of geniculate ganglion (GG), petrosal ganglion (PG), and nucleus of the solitary tract (NST) WGA-positive neurons transmitting from sweet/umami taste- and bitter taste-receiving cells. We also constructed transgenic medaka fish whose amino acid-receiving (T1Rs-positive) and bitter taste-receiving (T2Rs-positve) cells were made to have the WGA, and succeeded in visualizing their taste-transmitting pathways.
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[Journal Article] The single pore residue Asp (523) in PKD2L1 deter-mines Ca^<2+> permeation of a PKD1L3/PKD2L1 complex.2011
Author(s)
Fujimoto, C., Ishimaru, Y., Katano, Y., Misaka, T., Yamasoba, T., Asakura, T., Abe, K.
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Journal Title
Biochem.Biophys.Res.Commun. 404
Pages: 946-951
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[Journal Article] Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion : case of a nonglycosylated mutant of miraculin, a taste-modifying protein.2011
Author(s)
Ito, K., Sugawara, T., Koizumi, A., Nakajima, K.I., Shimizu-Ibuka, A., Shiroishi, M., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Asakura, T., Misaka, T., Iwata, S., Kobayashi, T., Abe, K.
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Journal Title
Biotechnol.Lett. 33
Pages: 103-107
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[Journal Article] The human bitter taste receptor, hTAS2R16, discriminates slight differences in the configuration of disaccharides.2010
Author(s)
Sakurai, T., Misaka, T., Ueno, Y Ishiguro, M., Matsuo, S., Ishimaru, Y., Asakura, T., Abe, K.
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Journal Title
Biochem.Biophys.Res.Commun. 402
Pages: 595-601
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[Journal Article] Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.2010
Author(s)
Ito, K., Ito, S., Shimamura, T., Kawarasaki, Y., Abe, K., Misaka, T., Kobayashi, T., Iwata, S.
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Journal Title
Acta Crystallogr.Sect.F Struct.Biol.Cryst.Commun. 66
Pages: 1086-1088
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[Journal Article] Surface plasmon resonance analysis on interactions of food components with a taste epithelial cell model.2010
Author(s)
Miyano, M., Yamashita, H., Sakurai, T., Nakajima, K., Ito, K., Misaka, T., Ishimaru, Y., Abe, K., Asakura, T.
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Journal Title
J.Agric.Food Chem. 58
Pages: 11870-11875
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[Journal Article] Characterization of the beta-D-glucopyranoside binding site of the human bitter taste receptor hTAS2R16.2010
Author(s)
Sakurai, T., Misaka, T., Ishiguro, M., Masuda, K., Sugawara, T., Ito, K., Kobayashi, T., Matsuo, S., Ishimaru, Y., Asakura, T., Abe, K.
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Journal Title
J.Biol.Chem. 285
Pages: 28373-28378
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[Journal Article] Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae.2010
Author(s)
Ishimaru, Y., Katano, Y., Yamamoto, K., Akiba, M., Misaka, T., Roberts, R.W., Asakura, T., Matsunami H., Abe, K.
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Journal Title
FASEB J. 24
Pages: 4058-4067
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[Journal Article] Amiloride reduces the sweet taste intensity by inhibiting the human sweet taste receptor.2010
Author(s)
Imada, T., Misaka, T., Fujiwara, S., Okada, S., Fukuda, Y., Abe, K.
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Journal Title
Biochem.Biophys.Res.Commun. 397
Pages: 220-225
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[Journal Article] Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin.2010
Author(s)
Ito, K., Sugawara, T., Koizumi, A., Nakajima, K., Shimizu-Ibuka, A., Shiroishi, M., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Asakura, T., Misaka, T., Iwata, S., Kobayashi, T., Abe, K.
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Journal Title
Biochim.Biophys.Acta 1800
Pages: 986-992
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[Journal Article] Photoactive ligands probing the sweet taste receptor.Design and synthesis of highly potent diazirinyl d-phenylalanine derivatives.2010
Author(s)
Masuda, K., Koizumi, A., Misaka, T., Hatanaka, Y., Abe, K., Tanaka, T., Ishiguro, M., Hashimoto, M.
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Journal Title
Bioorg.Med.Chem.Lett. 20
Pages: 1081-1083
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[Journal Article] The Hsp90 inhibitor geldanamycin abrogates colocalization of eIF4E and 4E-T into stress granules and association of eIF4E with eIF4G.2009
Author(s)
Suzuki, Y., Minami, M., Suzuki, M., Abe, K., Zenno, S., Tsujimoto, M., Matsumoto, K., Minami, Y.
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Journal Title
J.Biol.Chem. 284
Pages: 35597-35604
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[Journal Article] pH-dependent structural change in neoculin with special reference to its taste-modifying activity.2009
Author(s)
Morita, Y., Nakajima, K., Iizuka, K., Terada, T., Shimizu-Ibuka, A., Ito, K., Koizumi, A., Asakura, T., Misaka, T., Abe, K.
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Journal Title
Biosci.Biotechnol.Biochem. 73
Pages: 2552-2555
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[Journal Article] Spatial differences in molecular characteristics of the pontine parabrachial nucleus.2009
Author(s)
Maeda, N., Onimura, M., Ohmoto, M., Inui, T., Yamamoto, T., Matsumoto, I., Abe, K.
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Journal Title
Brain Res. 1296
Pages: 24-34
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[Journal Article] Acetic acid activates PKD1L3-PKD2L1 channel-A candidate sour taste receptor.2009
Author(s)
Ishii, S., Misaka, T., Kishi, M., Kaga, T., Ishimaru, Y. Abe, K.
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Journal Title
Biochem.Biophys.Res.Commun. 385
Pages: 346-350
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[Journal Article] Functional organization of the rodent parabrachial nucleus.2009
Author(s)
Yamamoto, T., Takemura, M., Inui, T., Torii, K., Maeda, N., Ohmoto, M., Matsumoto, I., Abe, K.
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Journal Title
Ann.N.Y.Acad.Sci. 1170
Pages: 378-382
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[Journal Article] Flourescence- based optimization of human bitter taste receptor expression in Saccharomyces cerevisiae.2009
Author(s)
Sugawara, T., Ito, K., Shiroishi, M., Tokuda, N., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Misaka, T., Nomura, N., Murata, T., Abe, K., Iwata, S., Kobayashi, T.
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Journal Title
Biochem.Biophys.Res.Commun. 382
Pages: 704-710
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[Journal Article] pH-Dependent inhibition of the human bitter taste receptor hTAS2R16 by a variety of acidic substances.2009
Author(s)
Sakurai, T., Misaka, T., Nagai, T., Ishimaru, Y., Matsuo, S., Asakura T., Abe, K.
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Journal Title
J.Agric.Food Chem. 57
Pages: 2508-2514
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[Journal Article] Umami taste dysfunction in patients receiving radiotherapy for head and neck cancer.2009
Author(s)
Yamashita, H., Nakagawa, K., Hosoi, Y., Kurokawa, A., Fukuda, Y., Matsumoto, I., Misaka, T., Abe, K.
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Journal Title
Oral Oncol. 45
Pages: e19-23
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