Functions of myeloid-lectin receptors

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: INABA Kayo Kyoto University, 大学院・生命科学研究科, 教授 (00115792)
• Project Period (FY): 2008 – 2010
• Keywords: 動物 / 細胞 / 分子 / 炎症 / レクチン / マクロファージ / 樹状細胞

Research Abstract

We generated SIGNR3-specific mAbs and investigated SIGNR3 expression in vivo. SIGNR3 was expressed on a vast majority of CD11b^+CD11c^<low> myeloid cells in various tissues, such as blood, dermis, lung, spleen and lymph nodes. By the cell transfer experiments using fluorescenated Ly6C^<high>SIGNR3^- bone marrow monocytes, SIGNR3 expression was upregulated along with the decrease in Ly6C expression during the circulation. Upon arrival at the peripheral LNs through HEV, the inoculated cells differentiated into CD11b^+ DCs in a couple of days. These results indicate that some DCs, especially in the subcutaneous lymph nodes, are possibly replenished by Ly6C^<high> monocytes.
DCIR1 was detected mainly intracellularly in various cell types, but transferred onto the cell surface by the activation with LPS and TNF-α.

Research Products

• [Journal Article] C-type lectin SIGNR1 enhances cellular oxidative burst responses against C. albicans in cooperation with Dectin-1. (2011)
  • Author(s): Takahara, K., Tokieda, T., Nagaoka, K., Takeda, T., Kimura, Y., Inaba.K.
  • Journal Title: Eur.J. Immunol. Feb24 (Epub ahead of print)
• [Journal Article] Expression of the C-type lectin, SIGNR3, on subsets of dendritic cells, macrophages and monocytes using a new monoclonal antibody. (2010)
  • Author(s): Nagaoka, K., Takahara, K., Minamino, K., Takeda, T., Yoshida, Y., Inaba, K.
  • Journal Title: J Leu.Biol. 88(5) Pages: 913-924
  • Peer Reviewed
• [Journal Article] Rho-mDial pathway is required for adhesion, migration, and T cell stimulation in dendritic cells. (2010)
  • Author(s): Tanizaki, H., Egawa, G., Inaba, K., Honda, T., Nakajima, S., Sagita Moniaga, C., Otsuka, A., Ishizaki, T., Tomura, M., Watanabe, T., Miyachi, Y., Narumiya, S., Okada, T., Kabashima, K.
  • Journal Title: Blood 116(26) Pages: 5875-5884
  • Peer Reviewed
• [Journal Article] Ly49Q plays a crucial role in neutrophil polarization and migration by regulating raft trafficking. (2010)
  • Author(s): Sasawatari, S., Yoshizaki, M., Taya, C., Tazawa, A., Furuyama-Tanaka, K., Yonekawa, H., Dohi, T., Makrigiannis, A.P., Sasazuki, T., Inaba, K., Toyama-Sorimachi, N.
  • Journal Title: Immunity 31(2) Pages: 200-213
  • Peer Reviewed
• [Journal Article] Dual role for Id2 in chemical carcinogen-induced skin tumorigenesis. (2009)
  • Author(s): Tokuriki, A., Iyoda, T., Inaba, K., Ikuta, K., Fijimoto, S., Kumakiri, M., Yokota, Y.
  • Journal Title: Carcinogenesis. 30(9) Pages: 1645-1650
  • Peer Reviewed
• [Journal Article] CD8^+CD205^+splenic dendritic cells are specialized to induce Foxp3^+ regulatory T cells. (2008)
  • Author(s): Yamazaki,S., Dudziak, D., Heidkamp,F.D., Fiorese, C., Bonito, A.J., Inaba,K., Nussenzweig, M.D., Steinman, R.M.
  • Journal Title: J.Immunol. 181(10) Pages: 6923-6933
  • Peer Reviewed
• [Journal Article] CD8^+CD205^+ splenic dendritic cells are specialized to induce Foxp3^+regulatory T cells. (2008)
  • Author(s): Yamazaki, S., Dudziak D., Heidkamp, GF., Fiorese, C., Bonito, AJ., Inaba, K., Nussenzweig, MC., Steinman, RM.
  • Journal Title: J.Immunol 181(10) Pages: 6923-6933
  • Peer Reviewed
• [Journal Article] Serial assessment of immune status by circulating CD8^+effector T cell frequencies for posttransplant infectious complications. (2008)
  • Author(s): Umenoto, S., Ozawa, K., Egawa, H., Takada, Y., Sato, H., Teramukai, S., Kasahara, M., Ofura, K., Ono, M., Takai, K., Fukushima, M., Inaba, K., Tanaka, K.
  • Journal Title: Clin.Dev.Immunol. Pages: 2008:718386(13)
  • Peer Reviewed
• [Journal Article] Dendritic cells derived from thioredoxin binding protein-2 deficient mice attenuate Th1 responses. (2008)
  • Author(s): Son, A., Nakamura, H., Oka, S-I., Takenaka, M., Yoshihara, E., Liu, W.m Matsuo, Y., Kondo, N., Masutani, H., Ishii, Y., Iyoda, T., Inaba, K., Yodoi, J.
  • Journal Title: Eur.J.Immunol. 38(5) Pages: 1358-1367
  • Peer Reviewed
• [Journal Article] Invariant NKT cell anergy is induced by a strong TCR-mediated signal plus costimulation.
  • Author(s): Iyoda, T., Ushida, M., Kimura, Y., Minamino, K., Hayuka, A., Yokohata, S., Ehara, H., Kayo Inaba, K.
  • Journal Title: Int.Immunol. 22(11) Pages: 905-913
  • Peer Reviewed
• [Presentation] Role of SIGNR1 molecules in innate immune responses. (2010)
  • Author(s): Inaba K., Takahara, K.
  • Organizer: In "Adaptive and Innate Immune Responses to Neglected Tropical Diseases Conference".
  • Place of Presentation: San Diego, Ca.
  • Year and Date: 20100110-20100111
• [Presentation] 樹状細胞-基礎から臨床へ- (2010)
  • Author(s): 稲葉カヨ
  • Organizer: 第11回国際統合医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-18
• [Presentation] Is SIGNR3 a new marker to identify intermeidated cell type differentiating into dendritic cells? (2010)
  • Author(s): 稲葉カヨ
  • Organizer: In "Asia Pacific Regional Meeting"
  • Place of Presentation: Osaka
  • Year and Date: 2010-02-04
• [Presentation] 樹状細胞研究の動向と展望 (2010)
  • Author(s): 稲葉カヨ
  • Organizer: 第9回リウマチ性疾患研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-01-23
• [Presentation] Determination of polysaccharides on C.albicans mannoprotein recognized by human C-type lectin, DC-SIGN. (2009)
  • Author(s): Takahara, K., Arita, T., Shibata, N., Okawa, Y, Inaba K.
  • Organizer: In "The 9^<th> Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai, Japan
  • Year and Date: 20090908-20090911
• [Presentation] Role of SIGN-related molecules in innate immune responses. (2009)
  • Author(s): Takahara, K., Inaba K.
  • Organizer: International Symposium "Acquired Immunity and Glycobiology"
  • Place of Presentation: Kazusa, Chiba
  • Year and Date: 20090323-20090324
• [Presentation] 樹状細胞研究の動向・展望と臨床応用への課題 (2009)
  • Author(s): 稲葉カヨ
  • Organizer: 第84回北野学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-25
• [Presentation] 樹状細胞 (2009)
  • Author(s): 稲葉カヨ
  • Organizer: 日本免疫学会サマースクール2009
  • Place of Presentation: 淡路
  • Year and Date: 2009-07-17
• [Presentation] 樹状細胞:免疫応答制御の視点から (2009)
  • Author(s): 稲葉カヨ
  • Organizer: 第5回千葉基礎・臨床免疫セミナー
  • Place of Presentation: 千葉
  • Year and Date: 2009-01-09
• [Presentation] C-type lectins expressed on macrophages and dendritic cells. (2008)
  • Author(s): Inaba K., Takahara, K.
  • Organizer: In the 57^<th> Fall conference of the Korean Association of Immunologist.
  • Place of Presentation: Seoul, Korea
  • Year and Date: 20081113-20081114
• [Presentation] Subsets of macrophages and dendritic cells defined by newly established monoclonal antibody against SIGNR3. (2008)
  • Author(s): 稲葉カヨ
  • Organizer: In 8^<th> Awaji International Forum for Infection and Immunity
  • Place of Presentation: Awaji Island, Hyogo
  • Year and Date: 20080908-20080911
• [Presentation] Role of SIGNR1 in anti-microbial responses. (2008)
  • Author(s): Inaba K., Takahara, K.
  • Organizer: In U.S.-Japan Cooperative Medical Science Program : Workshop on Aging&Immunosenescence
  • Place of Presentation: San Francisco, CA
  • Year and Date: 20080718-20080721
• [Book] 樹状細胞研究のトピックス『炎症と免疫』特集IVol.18, No.5. (2010)
  • Author(s): 稲葉カヨ
  • Total Pages: 1-38
  • Publisher: 先端医学社
• [Book] Isolation of Dendritic Cells. Aug ; Chapter 3 : Unit3.7 (2009)
  • Author(s): Inaba K., Swiggard, W.J., Steinman, R.M., Romani, N., Schuler, G., Brinster, C.
  • Publisher: Curr Protoc Immunol.
• [Book] 樹状細胞『炎症・再生医学事典』 (2009)
  • Author(s): 稲葉カヨ
  • Total Pages: 42-45
  • Publisher: 朝倉書店
• [Book] 生体における樹状細胞の前駆細胞とその分化『肝胆膵』Vol.58, No.2 (2009)
  • Author(s): 長岡孝治、稲葉カヨ
  • Total Pages: 157-166
  • Publisher: アークメディア
• [Book] 樹状細胞:発見・同定の歴史を今に見る『実験医学・増刊:樹状細胞による免疫制御と臨床応用』 (2008)
  • Author(s): 稲葉カヨ
  • Total Pages: 18-26
  • Publisher: 羊土社
• [Book] 第7章抗原提示「免疫学イラストレイテッド原著第7版」(訳) (2008)
  • Author(s): 稲葉カヨ
  • Total Pages: 145-162
• [Remarks] ホームページ等
  • URL: http://zoo.zool.kyoto-u.ac.jp/imm/lab.html