2010 Fiscal Year Final Research Report
Identification of host cell factors involved in diphtheria toxin sensitivity by using shRNA library.
| Project/Area Number |
20390127
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
MEKADA Eisuke 大阪大学, 微生物病研究所, 教授 (20135742)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ジフテリア毒素 / shRNA / エンドサイトーシス / 宿主因子 / 網羅的遺伝子同定法 |
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| Research Abstract |
We performed a large scale screening of genes encoding host cell factors involved in cell sensitivity to diphtheria toxin by using shRNA library. Total 11 genes were identified as genes which are possibly involved in DT sensitivity. Among them, genes encoding enzymes in cholesterol biosynthesis pathway were confirmed to be involved in DT sensitivity.
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[Journal Article] HB-EGF and PDGF mediate reciprocalinteractions of carcinoma cells withcancer-associated fibroblasts tosupport progression of uterine cervicalcancers2011
Author(s)
Murata T, Mizushima H, Chinen I, Moribe H, Yagi S, Hoffman RM, Kimura T, Yoshino K, Ueda Y, Enomoto T, Mekada E
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Journal Title
Cancer Research
Volume: 71
Pages: 6633-6642
Peer Reviewed
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[Journal Article] A novelanti-human HB-EGF monoclonal antibodywith multiple antitumor mechanismsagainst ovarian cancer cells.2011
Author(s)
Miyamoto S, Iwamoto R, Furuya A, Takahashi K, Sasaki Y, Ando H, YotsumotoF, Yoneda T, Hamaoka M, Yagi H, MurakamiT, Hori S, Shitara K, Mekada E
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Journal Title
Clinical Cancer Research
Volume: 17
Pages: 6733-6741
Peer Reviewed
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[Journal Article] Assessment ofHB-EGF levels in peritoneal fluid andserum of ovarian cancer patients usingELISA2011
Author(s)
Hikita S, Yotsumoto F, Fukami T, Horiuchi S, Sanui A, Miyata K, Nam SO, Tsujioka H, Ueda T, Shirota K, YoshizatoT, Maeda K, Ishikawa T, Okuno Y, Kuroki M, Mekada E, Miyamoto S
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Journal Title
Anticancer Res
Volume: 31
Pages: 2553-2559
Peer Reviewed
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[Journal Article] Thefusing ability of sperm is bestowed byCD9-containing vesicles released fromeggs in mice2008
Author(s)
Miyado K, Yoshida K, Yamagata K, Sakakibara K, Okabe M, Wang X, MiyamotoK, Akutsu H, Kondo T, Takahashi Y, BanT, Ito C, Toshimori K, Nakamura A, ItoM, Miyado M, Mekada E, Umezawa A
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Journal Title
Proc Natl Acad Sci USA
Volume: 105
Pages: 12921-12926
Peer Reviewed
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[Journal Article] Double deficiency oftetraspanins CD9 and CD81 alters cellmotility and protease production of macrophages and causes COPD-likephenotype in mice2008
Author(s)
Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, Suzuki M, Minami S, Iwasaki T, Goya S, Kijima T, Kumagai T, Yoshida M, Osaki T, Komori T, Mekada E, Kawase I.
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Journal Title
J Biol Chem
Volume: 283
Pages: 26089-26097
Peer Reviewed
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[Journal Article] Possible involvement of CD81 in acrosome reaction of sperm inmice2008
Author(s)
Tanigawa M, Miyamoto K, KobayashiS, Sato M, Akutsu H, Okabe M, MekadaE, Sakakibara K, Miyado M, Umezawa A, Miyado K
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Journal Title
Mol Reprod Dev
Volume: 75
Pages: 150-155
Peer Reviewed
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