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2010 Fiscal Year Final Research Report

A new strategy for antibody-therapy against infectious diseases and bacterial terrorism using lymphocyte-chip

Research Project

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Project/Area Number 20390286
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Infectious disease medicine
Research InstitutionUniversity of Toyama

Principal Investigator

MURAGUCHI Atsushi  University of Toyama, 大学院・医学薬学研究部, 教授 (20174287)

Co-Investigator(Kenkyū-buntansha) KISHI Hiroyuki  富山大学, 大学院・医学薬学研究部, 准教授 (60186210)
OZAWA Tatsuhiko  富山大学, 大学院・医学薬学研究部, 助教 (10432105)
KITAJIMA Isao  富山大学, 大学院・医学薬学研究部, 教授 (50214797)
NIKAIDO Toshio  富山大学, 大学院・医学薬学研究部, 教授 (50180568)
Project Period (FY) 2008 – 2010
Keywordsリンパ球チップ / 抗体医療 / 抗体遺伝子
Research Abstract

We developed ISAAC (Immunospot assay for antibody-secreting cell) method that can detect antibody-secreting cells in a microwell using murine system. We then succeeded in generating high-quality human monoclonal antibodies for hepatitis B virus and influenza virus from vaccinated healthy volunteers using the ISAAC method in a very short time ; within one week. We further developed an in vitro assay system to examine the HBV-neutralizing activity of human anti-HBs antibodies and clarified the relationship between neutralizing activity of human mAbs and epitopes of HBs antigen.

  • Research Products

    (22 results)

All 2011 2010 2009 2008

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (15 results)

  • [Journal Article] Generation of TRAIL-receptor 1-specific human monoclonal Ab by a combination of immunospot array assay on a chip and human Ab-producing mice.2010

    • Author(s)
      Jin A., Ozawa T., Tajiri K., Lin Z., Obata T., Ishida I., Kishi H., Muraguchi A.
    • Journal Title

      Eur.J.Immunol. 40

      Pages: 3591-3593

    • Peer Reviewed
  • [Journal Article] Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine.2010

    • Author(s)
      Tajiri K., Ozawa T., Jin A., Tokimitsu Y., Minemura M., Kishi H., Sugiyama T., Muraguchi A.
    • Journal Title

      Antiviral.Res. 87

      Pages: 40-49

    • Peer Reviewed
  • [Journal Article] Post-translational modification of TRAIL receptor type 1 on various tumor cells and the susceptibility of tumors to TRAIL-induced apoptosis.2010

    • Author(s)
      Lin Z., Jin A., Ozawa T., Tajiri K., Obata T., Ishida I., Jin F., Kishi H., Muraguchi A.
    • Journal Title

      Biochem.Biophys.Res.Commun. 395

      Pages: 251-257

    • Peer Reviewed
  • [Journal Article] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood.2009

    • Author(s)
      Jin A.-S., Ozawa T., Tajiri K., Obata T., Kondo S., Kinoshita K., Kadowaki S., Takahashi K., Sugiyama T., Kishi H., Muraguchi A.
    • Journal Title

      Nat.Med. 15

      Pages: 1088-1092

    • Peer Reviewed
  • [Journal Article] Identification of antigen-specific B cells by concurrent monitoring of intracellular Ca^<2+> mobilization and antigen binding with microwell array chip system equipped with a CCD imager.2009

    • Author(s)
      Kinoshita K., Ozawa T., Tajiri K., Kadowaki S., Kishi H., Muraguchi A.
    • Journal Title

      Cytometry Part A 75A

      Pages: 682-687

    • Peer Reviewed
  • [Journal Article] SFMAC : A novel method for analyzing multiple parameters on lymphocytes with a single fluorophore in cell-microarray system.2009

    • Author(s)
      Tajiri K., Kishi H., Ozawa T., Sugiyama T., Muraguchi A.
    • Journal Title

      Cytometry Part A 75A

      Pages: 282-288

    • Peer Reviewed
  • [Journal Article] MAC-CCD system : a novel lymphocyte microwell-array chip system equipped with CCD scanner to generate human monoclonal antibodies against influenza virus.2009

    • Author(s)
      Ozawa T., Kinoshita K., Kadowaki S., Tajiri K., Kondo S., Honda R., Ikemoto M., Piao L.-X., Morisato A., Fukurotani K., Kishi H., Muraguchi A.
    • Journal Title

      Lab on a Chip 9

      Pages: 158-163

    • Peer Reviewed
  • [Presentation] Characterization of human anti-influenza M2 antibody obtained from peripheral blood of vaccinated volunteer.2011

    • Author(s)
      Muraguchi A., Ozawa T., Jin A., Tajiri K., Takemoto M., Okuda T., Shiraki K., Kishi H.
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Keystone, USA.
    • Year and Date
      20110206-20110211
  • [Presentation] Isolation and characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus.2010

    • Author(s)
      Ozawa T., Jin A., Tajiri K., Takemoto M., Okuda T., Shiraki K., Kishi H., Muraguchi A.
    • Organizer
      Cold Spring Harbor Conferences Asia
    • Place of Presentation
      Suzhou, China.
    • Year and Date
      20101107-20101110
  • [Presentation] Enhancement of TRAIL-induced apoptosis with novel TRAIL-receptor 1 specific human mAbs generated by Immunospot array assay on a chip.2010

    • Author(s)
      Jin A., Ozawa T., Tajiri K., Lin Z., Kishi H., Muraguchi A.
    • Organizer
      Cold Spring Harbor Conferences Asia
    • Place of Presentation
      Suzhou, China.
    • Year and Date
      20101107-20101110
  • [Presentation] Enhancement of TRAIL-induced apoptosis with novel TRAIL-R1-specific human mAbs generated by immunospot array assay on a chip.2010

    • Author(s)
      Kishi H., Jin A., Ozawa T., Tajiri K., Lin Z., Obata T., Ishida I., Muraguchi A.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe.
    • Year and Date
      20100822-20100827
  • [Presentation] Characterization of human anti-influenza M2 antibody obtained from peripheral blood of vaccinated volunteer.2010

    • Author(s)
      Ozawa T., Jin A., Tajiri K., Shiraki K., Kishi H., Muraguchi A.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe.
    • Year and Date
      20100822-20100827
  • [Presentation] Detection of antigen-specific T-cell response on the single cell-basis with microwell array chip.2010

    • Author(s)
      Kishi H., Tajiri K., Hatakeyama S., Kobayashi E., Horii M., Jin A., Ozawa T., Muraguchi A.
    • Organizer
      4th Measuring Antigen-Specific Immune Response (MASIR) Conference
    • Place of Presentation
      Mykonos, Greece.
    • Year and Date
      20100609-20100612
  • [Presentation] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood.2009

    • Author(s)
      小澤龍彦, 金艾順, 田尻和人, 小幡勤, 近藤佐千子, 木下耕史, 門脇慎一, 高橋和郎, 杉山敏郎, 岸裕幸, 村口篤
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20091209-20091212
  • [Presentation] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood.2009

    • Author(s)
      金艾順, 小澤龍彦, 田尻和人, 岸裕幸, 村口篤
    • Organizer
      IBC's 20th Annual International Conference Antibody Engineering
    • Place of Presentation
      San Diego, USA.
    • Year and Date
      20091206-20091210
  • [Presentation] 単一細胞レベルにおけるB細胞受容体シグナル解析2009

    • Author(s)
      岸裕幸, 小澤龍彦, 田尻和人, 村口篤
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      20091202-20091204
  • [Presentation] Isolation of human anti-influenza M2 antibody from peripheral blood of healthy volunteer.2009

    • Author(s)
      小澤龍彦, 田尻和人, 金艾順, 岸裕幸, 村口篤
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      20091202-20091204
  • [Presentation] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood.2009

    • Author(s)
      金艾順, 小澤龍彦, 田尻和人, 岸裕幸, 村口篤
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      20091202-20091204
  • [Presentation] Extracellular loop domain of small (S)-HBsAg play a major role in preventing the infection of HBV : the result from omprehensive analysis of monoclonal antibodies obtained from S-HBsAg-vaccinated healthy individuals.2009

    • Author(s)
      Tajiri K., Ozawa T., Jin A.-S., Tokimitsu Y., Nakayama Y., Minemura M., Kishi H., Sugiyama T., Muraguchi A.
    • Organizer
      The 8th JSH Single Topic Conference
    • Place of Presentation
      Tokyo.
    • Year and Date
      20091121-20091122
  • [Presentation] B-cell receptor signal analysis at single cell levels using a microwell-array chip.2009

    • Author(s)
      Kinoshita K., Kishi H., Fukurotani K., Honda R., Ozawa T., Tajiri K., Muraguchi A.
    • Organizer
      Cold Spring Harbor Meeting
    • Place of Presentation
      New York.
    • Year and Date
      20091111-20091114
  • [Presentation] Microwell arrays for detecting single, antigen-specific B-lineage cells.2008

    • Author(s)
      岸裕幸
    • Organizer
      IBC's 19th Annual International Conference Antibody Engineering
    • Place of Presentation
      San Diego.
    • Year and Date
      20081207-20081211
  • [Presentation] マイクロウェルアレイチップを用いた抗原特異的T細胞の検出2008

    • Author(s)
      岸裕幸, 田尻和人, 小澤龍彦, 金艾順, 村口篤
    • Organizer
      第38回日本免疫学会総会・学術集会
    • Place of Presentation
      京都
    • Year and Date
      20081201-20081203

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Published: 2012-01-26   Modified: 2016-04-21  

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