2010 Fiscal Year Final Research Report
Establishment of a method inducing embryonic stem cells to differentiate to hematopoietic stem cells (HSC) for therapeutic HSC transplantation
| Project/Area Number |
20390291
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
TSUJI Kohichiro The University of Tokyo, 医科学研究所, 准教授 (50179991)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
EBIHARA Yasuhiro 東京大学, 医科学研究所, 助教 (40302608)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | ヒト胚性幹細胞(ES細胞) / 造血幹細胞 / ストローマ細胞 |
|---|
| Research Abstract |
We challenged to establish a method inducing human embryonic stem (ES) cells to differentiate into hematopoietic stem cells without animal cells and serum for their clinical application. In brief, we first transferred human ES cells onto gelatin-coated culture dishes and cultured with 5% human platelet lysate or serum-containing media to induce their differentiation into stromal cells. Then, human ES cells were cocultured with the estabshished stromal cells under animal serum-free condition for 2 weeks to generate multipotent hematopoietic cells.Thus, we succeeded the establishment of a novel method inducing human ES cells to differentiate into multipotent hematopoietic cells without anima cells and seru,.
|
-
[Journal Article] Sero-prevalence of pandemic (H1N1) 2009 influenza A virus among schoolchildren and their parents inTokyo, Japan.2011
Author(s)
Iwatsuki-Horimoto K, Horimoto T, Tamura D, Kiso M, Kawakami E, Hatakeyama S, Ebihara Y, Koibuchi T, Fujii T, Takahashi K, Shimojima M, Sakai-Tagawa Y, Ito M, Sakabe S, Iwasa A, Takahashi K, Ishii T, Gorai T, Tsuji K, Iwamoto A, Kawaoka Y.
-
Journal Title
J Clinic Microbiol (掲載確定)
Peer Reviewed
-
-
-
[Journal Article] A 5'untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model.2010
Author(s)
Nagamachi A, Htun PW, Ma F, Miyazaki K, Yamasaki N, Kanno M, Inaba T, Honda Z, Okuda T, Oda H, Tsuji K, Honda H H(*equally contributed)
-
Journal Title
Dev Biol 345
Pages: 226-236
Peer Reviewed
-
-
-
-
[Journal Article] Cardiovascular toxicity of cryopreserved cord blood cell infusion.2008
Author(s)
Konuma T, Konuma T, Ooi J, Takahashi S, Tomonari A, Tsukada N, Kobayashi T, Sato A, Kato S, Kasahara S, Ebihara Y, Nagamura-Inoue T, Tsuji K, Tojo A, Asano S.
-
Journal Title
Bone Marrrow Transplant 41
Pages: 861-865
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Remarks] ホームページ等
-
-
-
-
-
