2010 Fiscal Year Final Research Report
Pediatric hematological disorder caused by in utero facors
| Project/Area Number |
20390302
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MIZUTANI Shuuki Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 教授 (60126175)
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| Co-Investigator(Kenkyū-buntansha) |
高木 正稔 東京医科歯科大学, 講師 (10406267)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ATM / BCR / ABL / CML / γδT細胞 / LOH / DNA damage |
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| Research Abstract |
Genetic and environmental factors on the fetus were studied for the possible causative mechanism of childhood leukemia. As the experimental model etoposide was injected into pregnant mice and DNA damage response was monitored in mononuclear cells in fetal liver and bone marrow of the mother. As the result DNA damage was much more extensive in fetal liver than the corresponding mother's bone marrow. Oncogenic effect by active oncogene was analyzed in ATM+/+ and ATM+/- mice. As the result haploinsufficiency of ATM was shown to cause higher frequency of leukemia than wild type background. This was the first evidence to demonstrate the biological effect of haploinsufficiency of ATM in vivo.
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