2010 Fiscal Year Final Research Report
Analysis of cell nuclear dynamics in thermotolerance development
| Project/Area Number |
20390330
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Nara Medical University |
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Principal Investigator |
TAKAHASHI Akihisa Nara Medical University, 先端科学研究指導者育成ユニット, 准教授 (60275336)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 放射線治療学 / 温熱治療 / 温熱耐性 / DNA二本鎖切断 / 細胞核ダイナミクス |
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| Research Abstract |
After heat treatment at 45.5℃ for 20 min, DNA DSB recognizing proteins were partially localized with γH2AX foci. Here we show that there were slight differences in the localization pattern among these proteins, such as a disappearance from the nucleus (phospho-ATM) and translocation to the cytoplasm (phospho-NBS1) at 30 min after heat treatment, and some foci (phospho- DNA-PKcs and phospho-Chk2) appeared at 8 h after heat treatment. In addition, we demonstrated that not NHEJ deficient cells but HR deficient cells is heat-sensitivity compared with these proficient cells. Polβ functions as a critical elements involved in thermotolerance. Moreover, we indicated that heat-induced foci of γH2AX and these DNA DSB recognizing proteins were suppressed in thermotolerance development.
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