2011 Fiscal Year Final Research Report
Analysis of molecular and cellular actions of general anesthetics on inhibitory synaptic transmission using genetically altered animals.
| Project/Area Number |
20390412
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Wakayama Medical University (2011)
Gunma University (2008-2010) |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2011
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| Keywords | GABA / 抑制シナプス / 鎮静 / 疼痛 / 遺伝子改変マウス / イオンチャネル / 神経毒性 / トランスポーター |
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| Research Abstract |
Genetically altered mice are very useful to analyze the molecular and cellular mechanisms of actions of general anesthetics and analgesics. Using GFP knock-in mice, we examined the effects of sevoflurane exposure to the developing brain, focusing on GABAergic neurons. In addition, we also investigated the possible influence of downregulation of vesicular GABA transporter(VGAT) on pain threshold and behavioral responses in mice. Our results provide genetic, behavioral and electrophysiological evidence that reduction of VGAT-mediated inhibitory drive in the spinal cord alters very specific forms of sensory processing : those related to pain processing.
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