Mechanism for maintenance of homeostasis of several functions by connexin 43

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390470
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MORITA Ikuo Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 教授 (60100129)
• Co-Investigator(Kenkyū-buntansha): NAKAHAMA Kenichi 東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (60281515) ; AKIYOSHI Kazunari 京都大学, 工学系研究科, 教授 (90201285) ; 市野瀬 志津子 東京医科歯科大学, 医歯科学究支援センター, 助教 (60014156) ; 小野寺 光江 東京医科歯科大学, 歯学部, 講師 (50376703)
• Project Period (FY): 2008 – 2010
• Keywords: コネキシン / ギャップ結合 / 薬物導入法 / annular gap / 細胞分化 / リポソーム

Research Abstract

To clarify a role of connexin 43 (Cx43) in vivo, we produced the substrate in which only two cells are able to be adjacent, the mutated Cx43 proteins, and Cx43 integrated liposomes. By using these techniques, it is demonstrated that the amino-acid sequence between C325 and C342 in Cx43 protein and Caveolin-1 are involved in the incorporation of Cx43-plaques. These findings show a novel mechanism for cell-cell interaction and takes part in the maintenance of homeostasis in several functions.

Research Products

• [Journal Article] TGF-b regulates isoform switching of FGF receptors and epithelial-mesenchymal transition. (2011)
  • Author(s): Shirakihara T, Horiguchi K, Miyazawa K, Ehata E, Shibata T, Morita I, Miyazono K, Saitoh M.
  • Journal Title: EMBO J 30(4) Pages: 783-789
• [Journal Article] Preparation of connexin43-integrated giant liposomes by a baculovirus expression-liposome fusion method. (2010)
  • Author(s): Kamiya K, Tsumoto K, Arakawa S, Shimizu S, Morita I, Yoshimura T, Akiyoshi K.
  • Journal Title: Biotechnology and Bioengineering 107(5) Pages: 836-843
• [Journal Article] Direct integration of cell-free synthesized connexin-43 into liposomes and hemichannel formation. (2010)
  • Author(s): Moritani, Y, Nomura S, Morita I, Akiyoshi K.
  • Journal Title: FEBS Journal. 277(16) Pages: 3343-3352
• [Journal Article] Therapeutic Angiogenesis by Implantation of a Capillary Structure Constituted of Human Adipose Tissue Microvascular Endothelial Cells. (2010)
  • Author(s): Yoshida T, Komaki M, Hattori H, Negishi J, Kishida A, Morita I, Abe M.
  • Journal Title: Arterioscler Thromb Vasc Biol. 30(7) Pages: 1300-1306
• [Journal Article] Pigpen, a nuclear coiled body component protein, is involved in angiogenesis. (2010)
  • Author(s): Yoshida T, Sato Y, Morita I, Abe M.
  • Journal Title: Cancer Sci. 101(5) Pages: 1170-1176
• [Journal Article] Transcriptome Remodeling in Hypoxic Inflammation. (2010)
  • Author(s): Safronova O, Morita I
  • Journal Title: J Dent Res.(Review) 89(5) Pages: 430-444
• [Journal Article] Cathepsin L in bone marrow-derived cells is required for retinal and choroidal neovascularization. (2010)
  • Author(s): Shimada N, Ohno-Matsui K, Iseki S, Koike M, Uchiyama Y, Wang J, Yoshida T, Sato T, Peters C, Mochizuki M, Morita I.
  • Journal Title: Amer.J Pathol. 176(5) Pages: 2571-2580
• [Journal Article] The effects of polyunsaturated fatty acids and their metabolites on osteoclastogenesis in vitro. (2010)
  • Author(s): Yuana J, Akiyama M, Nakahama K, Sato T, Uematsu H, Morita I
  • Journal Title: Prostaglandins and Other Lipid Mediators 92 Pages: 85-90
• [Journal Article] Increased Bone Mass in Adult Prostacyclin Deficient Mice. (2010)
  • Author(s): Nakalekha C, Yokoyama C, Miura Y, Alles N, Aoki K, Ohya K, Morita I.
  • Journal Title: J.Endocrinology 204(2) Pages: 125-133
• [Journal Article] Implantation of capillary structure engineered by optical lithography improves hindlimb ischemia in mice (2010)
  • Author(s): Akahori T, Kobayashi A, Komaki M, Hattori H, Kahahama K, Ichinose S, Abe M, Takeda S, Morita I.
  • Journal Title: Tissue Engendering, Part A. 16(3) Pages: 953-959
• [Journal Article] Potential enhancement of osteoclastogenesis by severe acute 3 respiratory syndrome coronavirus 3a/X1 protein (2009)
  • Author(s): Obitsu S, Hironori NA, Hasegawa A, Nakahama K, Morita I, Nishigaki N, Hayashi, Masuda T, Kannagi M.
  • Journal Title: Arch Virol 154(9) Pages: 1457-1464
• [Journal Article] Transcriptional Pathways in Hypoxic Inflammation (Review) (2009)
  • Author(s): Safronova O, Ikuo Morita.
  • Journal Title: Recent Advances and Research Updates. 10 Pages: 45-149, 2009
• [Journal Article] Regulation of chemokine gene expression by hypoxia via cooperative activation of NFkB and histone deacetylase. (2009)
  • Author(s): Safronova O, Pluemsampant S, Nakahama K, Morita I
  • Journal Title: Int J Biochem Cell Biology 41(11) Pages: 2270-2280
• [Journal Article] Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes. (2009)
  • Author(s): Kaneda M, Nomura, MS, Ichinose S, Kondo S, Nakahama k, Akiyoshi K, Morita I.
  • Journal Title: Biomaterials 30(23-24) Pages: 3971-3977
• [Journal Article] Amyloid-β up-regulates complement factor B in retinal pigment epithelial cells through cytokines released from recruited macrophages/microglia ; Another mechanism of complement activation in age-related macular degeneration (2009)
  • Author(s): Wang J, Ohno-Matsui K, Yoshida T, Shimada N, Ichinose S, Sato T, Mochizuki M, Morita I.
  • Journal Title: J Cell Phsiol 220(1) Pages: 119-128
• [Journal Article] The steady-state expression of connexin43 is maintained by the PI3K/Akt in osteoblasts. (2009)
  • Author(s): Bhattacharjee R, Kaneda M, Nakahama K, Morita I.
  • Journal Title: Biochem Biophys Res Commun. 382(2) Pages: 440-444
• [Journal Article] The reduction in pigment epithelium-derived factor is a sign of malignancy in ovarian cancer expressing low-level of vascular endothelial growth factor. (2009)
  • Author(s): Tsuchiya T, Nakahama K, Asakawa Y, Maemura T, Tanaka M, Takeda S, Morita M, Morita I
  • Journal Title: Gyncol Endocrinol 25(2) Pages: 104-109
• [Journal Article] The role of Mac-1 (CD11b/CD18) in osteoclast differentiation induced by receptor activator of nuclear factor- B ligand. (2008)
  • Author(s): Hayashi H, Nakahama K, Sato T, Tuchiya T, Asakawa Y, MaemuraT, Tanaka M, Morita I
  • Journal Title: FEBS Letters 82(21-22) Pages: 3243-3248
• [Journal Article] Vascular endothelial growth factor promotes proliferation and function of hepatocyte-like cells in embryoid bodies formed from mouse embryonic stem cells. (2008)
  • Author(s): Fujimori H, Asahina K, Shimizu-Saito K, Ikeda R, Tanaka Y, Teramoto K, Morita I, Teraoka H.
  • Journal Title: J.Hepatol 48(6) Pages: 962-973
• [Journal Article] Altered function of factor I caused by amyloid beta: implication for pathogenesis of age-related macular degeneration from Drusen. (2008)
  • Author(s): Wang J, Ohno-Matsui K, Yoshida T, Kojima A, Shimada N, Nakahama K, Safranova O, Iwata N, Saido TC, Mochizuki M, Morita I.
  • Journal Title: J Immunol. 181(1) Pages: 712-720
• [Journal Article] Vitamin K(2) suppresses malignancy of HuH7 hepatoma cells via inhibition of connexin 43. (2008)
  • Author(s): Kaneda M, Zhang D, Bhattacharjee R, Nakahama K, Arii S, Morita I.
  • Journal Title: Cancer Lett 263(1) Pages: 53-60
• [Journal Article] A comparison of the tube forming potentials of early and late endothelial progenitor cells. (2008)
  • Author(s): Mukai N, Akahori T, Komaki M, Li Q, Kanayasu-Toyoda T, Ishii-Watabe A, Kobayashi A, Yamaguchi T, Abe M, Amagasa T, Morita I.
  • Journal Title: Exp Cell Res 314(3) Pages: 430-440
• [Journal Article] Connexin 43 contributes to differentiation of retinal pigment epithelial cells via cyclic AMP signaling. (2008)
  • Author(s): Kojima A, Nakahama K, Ohno-Matsui K, Shimada N, Mori K, Iseki S, Sato T, Mochizuki M, Morita I.
  • Journal Title: Biochem Biophys Res Commun 366(2) Pages: 532-538
• [Journal Article] Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation. (2008)
  • Author(s): Huang, C, Wang J, Kikkawa U, Mukai H, Shen M, Morita I, Chen B, Chang WC
  • Journal Title: Oncogene 27(17) Pages: 2422-2429
• [Journal Article] Protein kinase CK2 is a key activator of histone deacetylase in hypoxia-associated tumors. (2008)
  • Author(s): Pluemsampant S, Safronova O, Nakahama K, Morita I.
  • Journal Title: Int J Cancer 122(2) Pages: 333-341
• [Journal Article] Cementum protein 1 (CEMP1) induces a cementoblastic phenotype and reduces osteoblastic differentiation in periodontal ligament cells.
  • Author(s): Komaki M, Iwasaki K, Arzate H, Sampath Narayanan A, Izumi Y, Morita I.
  • Journal Title: J Cell Physiol. in press
• [Journal Article] Topographic Analyses of Shape of Eyes with Pathologic Myopia by High-Resolution Three-Dimensional Magnetic Resonance Imaging.
  • Author(s): Moriyama M, Ohno-Matsui K, Hayashi K, Shimada N, Yoshida T, Tokoro T, Morita I.
  • Journal Title: Ophthalmology in press
• [Journal Article] Amyloidβ Enhances Migration of Endothelial Progenitor Cells by Upregulating CX3CR1 in Response to Fractalkine, Which May Be Associated With Development of Choroidal Neovascularization.
  • Author(s): Wang J, Ohno-Matsui K, Nakahama KI, Okamoto A, Yoshida T, Shimada N, Mochizuki M, Morita I.
  • Journal Title: Arterioscler Thromb Vasc Biol. in press
• [Journal Article] Cell-printing and transfer technology applications for bone defects in mice.
  • Author(s): Tsugawa J, Komaki M, Yoshida T, Nakahama K, Amagasa T, Morita I.
  • Journal Title: J Tissue Eng Regen Med in press
• [Presentation] ギャップジャンクションを介した破骨細胞分化抑制作用の検討 (2010)
  • Author(s): 中浜健一、小沢仁、鈴木岳史、森田育男
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-20101210
• [Presentation] ギャップ結合の調節機序とDDSへの応用創剤フォーラム (2009)
  • Author(s): 森田育男
  • Organizer: 第15回シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-10-23
• [Presentation] The steady-state expression level of connexin43 is maintained by the PI3K/Act pathway (2009)
  • Author(s): Bhattacharjee R, Kaneda M, Nakahama K, Morita I
  • Organizer: 10th International Symposium on Mechanism of Vasodilatation
  • Place of Presentation: 宮城(松島)
  • Year and Date: 2009-06-01
• [Presentation] The role of Mac-1 (CD11/CD18) in osteoclast differentiation induced by receptor activator of nuclear factor-κB ligand (RANKLE). (2008)
  • Author(s): Nakahama K, Hayashi H, Sato T, Morita I
  • Organizer: 48th The American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20081213-20081217
• [Presentation] Phosphatidylinositol 3- kinase akt regulates connexin43 expression through mRNA stability osteoblasts. (2008)
  • Author(s): Bhattacharjee R, Kaneda M, Nakahama K, Morita I
  • Organizer: 48th The American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20081213-20081217
• [Presentation] Roles of prostacyclin on bone metabolism. (2008)
  • Author(s): Chalida N, Yokoyama C, Miura H, Alles NR, Aoki K,Ohya k, Morita I
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-20081212
• [Presentation] Analysis of acceleratedosteoclast formation by metabolic acidosis. (2008)
  • Author(s): Kato K, Morita I
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-20081212
• [Remarks] ホームページ等
  • URL: http://www.tmd.ac.jp/dent/cell/