Real time and in situ observation of actin structure in migrating leukocyte

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500402
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kawasaki University of Medical Welfare
• Principal Investigator: KATAOKA Noriyuki Kawasaki University of Medical Welfare, 医療技術学部, 准教授 (20250681)
• Co-Investigator(Kenkyū-buntansha): TACHIBANA Hiroyuki 川崎医療福祉大学, 医療技術学部, 講師 (00241216) ; KAJIYA Fumihiko 川崎医療福祉大学, 医療技術学部, 教授 (70029114) ; HIRAMATSU Osamu 川崎医療福祉大学, 医療技術学部, 准教授 (50208849)
• Co-Investigator(Renkei-kenkyūsha): HASHIMOTO Ken 川崎医科大学, 医学部, 講師 (80341080) ; TSUJIOKA Katsuhiko 川崎医科大学, 医学部, 元教授 (30163801)
• Project Period (FY): 2008 – 2010
• Keywords: 白血球 / 内皮細胞 / 情報伝達 / ナノバイオロジー・ナノメディスン

Research Abstract

It is well known that one of the critical events in early atherosclerosis is the recruitment of blood monocytes to proatherogenic vascular regions and subsequent transmigration across vascular endothelial cells. It is still unknown how monocytes infiltrate into between and underneath EC monolayer. To elucidate this phenomenon, direct observation of cytoskeletal structure and/or signaling molecules in migrating monocytes under the controlled culture condition is necessary. In this study, we have established the observation system of migration of monocytic cell line induced by chemoattractant from micropipette. We have estimated the migration of HL-60 and THP-1 seeded on gelatin or fibronectin, to fMLP or MCP-1. In our experiments, THP-1 cells seeded on fibronectin actively migrated to the tip of micropipette. fMLP was more effective than MCP-1. In addition, we have found that PECAM-1 in EC is one of the key molecules that controls the leukocytes trans-endothelial migration and SHP-2 exists in the upstream of signaling transduction.

Research Products

• [Journal Article] Monocyte trans-endothelial migration augments subsequent transmigratory activity with increased PECAM-1 and decreased VE-cadherin at endothelial junctions (2011)
  • Author(s): K.Hashimoto, N.Kataoka, E.Nakamura, K.Hagihara, M.Hatano. T.Okamoto, H.Kanouchi, Y.Minatogawa, S.Mohri, K.Tsujioka, F.Kajiya
  • Journal Title: International Journal of Cardiology vol.149 No.2 Pages: 232-239
  • Peer Reviewed
• [Journal Article] Experimental Models for Studying Molecular Dynamics of Platelet-Endothelial Cell Adhesion Molecule-1 and Tubulin during Mechanical Interactions between Monocytes Vascular Endothelial Cells (2010)
  • Author(s): K.Hashimoto, N.Kataoka, E.Nakamura, T.Okamoto, H.Kanouchi, Y.Minatogawa, K.Tsujioka, S.Mohri, F.Kajiya
  • Journal Title: Journal of Biomechanical Science and Engineering vol.5, No.4 Pages: 281-290
  • Peer Reviewed
• [Journal Article] Endothelial Barrier Protective Effect of FTY720 under the Hyperglycemic Condition : Involvement of Focal Adhesion Kinase, Small GTPases and Adherens Junction Proteins (2009)
  • Author(s): K.Sarai, K.Shikata, Y.Shikata, K.Omori, N.Watanabe, M Sasaki, S.Nishishita, J.Wada, N Goda, N Kataoka, H.Makino
  • Journal Title: American Journal of Physiology-Cell Physiology Vol.297 No.4 Pages: C945-54
  • Peer Reviewed
• [Presentation] 単球の内皮下浸潤によって誘発される次の単球浸潤の促進 (2010)
  • Author(s): 片岡則之
  • Organizer: 第25回生体・生理工学シンポジウム
  • Place of Presentation: 岡山大学
  • Year and Date: 2010-09-25
• [Presentation] Monocyte trans-endothelial migration induces PECAM-1 accumulation and VE-cadherin reduction at the surface of vascular endothelial cells (2010)
  • Author(s): 片岡則之
  • Organizer: 6th World Congress on Biomechanics
  • Place of Presentation: Singapore Suntec Convention Centre
  • Year and Date: 2010-08-05
• [Presentation] Experimental Model for Studying Molecular Behavior in Endothelial Cells during Interaction with Monocytes (2010)
  • Author(s): 片岡則之
  • Organizer: 第49回日本生体医工学会
  • Place of Presentation: 大阪交流国際センター
  • Year and Date: 2010-06-26
• [Presentation] 細胞間隙を介する単球の浸潤により誘発される内皮細胞PECAM-1の浸潤部位への持続的集積 (2010)
  • Author(s): 橋本謙
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2010-01-09
• [Presentation] Role of dynamic recruitment of Endothelial PECAM-1 to Transmigrating Monocytes (2009)
  • Author(s): 片岡則之
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティ徳島
  • Year and Date: 2009-11-01
• [Presentation] Local Dynamic Recruitment of Endothelial PECAM-1 to Transmigrating Monocytes (2008)
  • Author(s): 片岡則之
  • Organizer: The 13th International Conference on Biomedical Engineering
  • Place of Presentation: Singapore Suntec Convention Centre
  • Year and Date: 2008-12-05
• [Remarks] ホームページ等