2010 Fiscal Year Final Research Report

Biomonitoring of water contamination based on analysis of the action potential of killifish

Research Project

Project/Area Number	20510157
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Social systems engineering/Safety system
Research Institution	Kyushu University
Principal Investigator	YOSHITOMI Kuniaki Kyushu University, 日本エジプト科学技術連携センター, 教授 (30150501)
Project Period (FY)	2008 – 2010
Keywords	メダカ / 水質汚染監視 / 活動電位
Research Abstract	In this study, based on the fact that respiratory impairment in fish serves as a good indicator of toxicant exposure stress, the electric field resulting from respiratory muscle action potentials were measured and analyzed. In order to detect the electric field accurately, titanium alloy electrodes were used. The toxicant used were potassium cyanide and insecticide Sumithion (fenitrothion) . Acute and sublethal toxic effect was indicated by increased breathing activity. The time characteristic of the power spectrum of the action potential showed that killifish is able to detect a cyanide concentration of 0.1mg/L and a Sumithion of 1mg/L in an hour.