Development of diagnostic technology for cancer detection using FRET and heparanase specificity

2010 Fiscal Year Final Research Report

• Project/Area Number: 20510200
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Tottori University
• Principal Investigator: TAMURA Jun-Ichi Tottori University, 地域学部, 教授 (30221401)
• Project Period (FY): 2008 – 2010
• Keywords: 糖鎖工学 / オリゴ糖 / がん / 診断薬

Research Abstract

We planned to synthesize heparan sulfate oligosaccharides labeled with fluorescencegroups to show FRET. This would be able to diagnose the cancer in early stage conveniently. In this research period we established a tereoselective synthetic route for the heparan sulfate tetrasaccharide in high yield. We are trying to equip the fluorescence groups on the heparin sulfate tetrasaccharide as a substrate.

Research Products

• [Journal Article] Down-regulation of Chondroitin 4-O-sulfotransferase-1 by Wnt Signaling Triggers Diffusion of Wnt-3a. (2011)
  • Author(s): S.Nadanaka, H.Kinouchi, K.Taniguchi-Morita, J.Tamura, H.Kitagawa
  • Journal Title: J.Biol.Chem. 286(6) Pages: 4199-4208
  • Peer Reviewed
• [Journal Article] Synthesis of the glycosaminoglycan-protein linkage tetraosyl peptide moieties of betaglycan, which serve as a hexosamine acceptor for enzymatic glycosyl transfer. (2010)
  • Author(s): J.Tamura, T.Nakamura-Yamamoto, Y.Nishimura, S.Mizumoto, J.Takahashi, K.Sugahara
  • Journal Title: Carbohydr. Res. 345 Pages: 2115-2123
  • Peer Reviewed
• [Journal Article] Anti-arthritic Activity of Synthesized Chondroitin Sulfate E Hexasaccharide. (2010)
  • Author(s): T.Matsumoto, A.Takahashi, A.Higuchi, K.Taniguchi-Morita, M.Miyasaka, J.Tamura
  • Journal Title: Arznei. Forsch. Drug Res. 60(12) Pages: 754-759
  • Peer Reviewed
• [Journal Article] Biosynthesis of Heparan Sulfate in EXT1-deficient cells. (2010)
  • Author(s): M.Okada, S.Nadanaka, N.Shoji, J.Tamura, H.Kitagawa
  • Journal Title: Biochem. J. 428(3) Pages: 463-471
  • Peer Reviewed
• [Journal Article] FAM20b is a Kinase that Phosphorylates Xylose in the Glycosaminoglycan-Protein Linkage Region. (2009)
  • Author(s): T.Koike, T.Izumikawa, J.Tamura, H.Kitagawa
  • Journal Title: Biochem. J. 421(2) Pages: 157-162
  • Peer Reviewed
• [Journal Article] Sulfation Patterns and the Amounts of Chondroitin Sulfate in Diamond squid, Thysanoteuthis rhombus. (2009)
  • Author(s): J.Tamura, K.Arima, A.Imazu, N.Tsutsumishita, H.Fujita, M.Yamane, Y.Matsumi
  • Journal Title: Biosci. Biotech. Biochem. 73(6) Pages: 1387-1391
  • Peer Reviewed
• [Journal Article] Chondroitin Sulfate E Fragments Enhance CD44 Cleavage and CD44-Dependent Motility in Tumor Cells. (2008)
  • Author(s): K.N.Sugahara, T.Hirata, T.Tanaka, S.Ogino, M.Takeda, H.Terasawa, I.Shimada, J.Tamura, G.B.ten Dam, T.H.van Kuppevelt, M.Miyasaka
  • Journal Title: Cancer Res. 68(17) Pages: 7191-7199
  • Peer Reviewed
• [Journal Article] 2-O-Phosphorylation of Xylose and 6-O-Sulfation of Galactose in the Protein Linkage Region of Glycosaminoglycans Influence the Glucuronyltransferase-I activity Involved in the Linkage Region's Synthesis. (2008)
  • Author(s): Y.Tone, L.C.Pedersen, T.Yamamoto, T.Izumikawa, H.Kitagawa, J.Nishihara, J.Tamura, M.Negishi, K.Sugahara
  • Journal Title: J.Biol.Chem. 283(24) Pages: 16801-16807
  • Peer Reviewed
• [Journal Article] Identification of Chondroitin Sulfate Glucuronyltransferase as Chondroitin Synthase-3 Involved in Chondroitin Polymerization : CHONDROITIN POLYMERIZATION IS ACHIEVED BY MULTIPLE ENZYME COMPLEXES CONSISTING OF CHONDROITIN SYNTHASE FAMILY MEMBERS. (2008)
  • Author(s): T.Izumikawa, T.Koike, S.Shiozawa, K.Sugahara, J.Tamura, H.Kitagawa
  • Journal Title: J.Biol.Chem. 283(17) Pages: 11396-11406
  • Peer Reviewed
• [Journal Article] Synthesis of Chondroitin Sulfate E Octasaccharide in a Repeating Region Involving an Acetamide Auxiliary. (2008)
  • Author(s): J.Tamura, Y.Nakada, K.Taniguchi, M.Yamane
  • Journal Title: Carbohydr. Res. 343 Pages: 39-47
  • Peer Reviewed
• [Presentation] ビオチンリンカーに結合したコンドロイチン硫酸オリゴ糖の合成と細胞との相互作用 (2011)
  • Author(s): 田村純一、川野愛美、加藤早紀、武田尚子、Herbert Geller、片桐康博
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] HNK-1硫酸基転移酵素はグリコサミノグリカン-タンパク質結合領域に硫酸基を転移する (2011)
  • Author(s): 橋口太志、水本秀二、西村裕子、田村純一、山田修平、菅原一幸
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-28
• [Presentation] 魚介類由来グリコサミノグリカンの含有量と組成分析 (2011)
  • Author(s): 松下健也、奥原隆司、林宏樹、湯村健、渡辺彩子、増田紳哉、石原幸雄、田村純一
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-27
• [Presentation] Synthesis of the glycosaminoglycan-protein linkage tetraosyl peptide moieties of betaglycan, which serve as a hexosamine acceptor for enzymatic glycosyl transfer. (2011)
  • Author(s): 田村純一
  • Organizer: PepCon-2011,(Invited)
  • Place of Presentation: Beijing (China)
  • Year and Date: 2011-03-23
• [Presentation] コンドロイチン硫酸Eの脂肪細胞分化抑制作用 (2010)
  • Author(s): 柘植亜衣子、保坂善真、田村純一、上原正人
  • Organizer: 第150回獣医学会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-17
• [Presentation] ナマコ由来糖鎖の脂肪細胞分化抑制活性 (2010)
  • Author(s): 保坂善真、田村純一、上原正人
  • Organizer: 第150回獣医学会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-17
• [Presentation] Synthesis of Heparan Sulfate Oligosaccharides for Heparanase Detection (2010)
  • Author(s): 武田尚子, 大瀧未来, 池田礼美奈, 南條美輪, 田村純一
  • Organizer: 25th International Carbohydrate Symposium
  • Place of Presentation: Makuhari (Japan)
  • Year and Date: 2010-08-04
• [Presentation] Synthesis of Oligosaccharides with Protected Sulfate Groups (2010)
  • Author(s): 松下健也, 船本真司, 田村純一
  • Organizer: 25th International Carbohydrate Symposium
  • Place of Presentation: Makuhari (Japan)
  • Year and Date: 2010-08-04
• [Presentation] Contents and Compositional Analyses of Glycosaminoglycans Derived from Fish (2010)
  • Author(s): J.Tamura, K.Arima, H.Fujita, M.Kitagaki, A.Nakamura, R.Hatto, Y.Hieda, S.Morimoto, S.Masuda, Y.Ishihara, S.Yoshioka, S.Yoshioka
  • Organizer: 25th International Carbohydrate Symposium
  • Place of Presentation: Makuhari (Japan)
  • Year and Date: 2010-08-04
• [Presentation] 亜臨界水処理による硫酸化多糖の低分子化 (2010)
  • Author(s): 森本稔、林哲也、森大貴、川本仁志、伊福伸介、田村純一、南三郎、斎本博之
  • Organizer: 第59高分子学会年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-05-26
• [Presentation] 植物毒-radicinin-の生合成-第4報? (2010)
  • Author(s): 鈴木大進、作野えみ、石原亨、田村純一、中島廣光
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 酵素活性検出用基質糖鎖の合成 (2010)
  • Author(s): 武田尚子、谷口-森田佳代、大瀧未来、池田礼美奈、南條美輪、田村純一
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] ビオチンリンカーに結合したコンドロイチン硫酸オリゴ糖の合成と特異タンパクとの相互作用 (2010)
  • Author(s): 田村純一、堤下奈緒、中尾康裕、川野愛美、灘中里美、北川裕之
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 位置特異的保護硫酸化オリゴ糖の合成 (2010)
  • Author(s): 松下健也、船本真司、田村純一
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 山陰産魚介類由来グリコサミノグリカンの含有量と組成分析 (2010)
  • Author(s): 藤田裕之、北垣雅大、中村歩、八東龍也、稗田優香、森本慎、川野愛美、田中晴奈、松下健也、増田紳哉、石原幸雄、吉岡忍、吉岡俊介、高森吉守、田村純一
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 水熱処理により低分子化されたコンドロイチン硫酸の硫酸基分布 (2010)
  • Author(s): 森大貴、林哲也、田村純一、殿井貴之、伊福伸介、森本稔、斎本博之
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-27
• [Presentation] コンドロイチンオリゴ糖への硫酸基の位置選択的導入 (2009)
  • Author(s): 田村純一、松下健也、武田尚子、船本真司
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 20090300
• [Presentation] Synthesis of glycosaminoglycan oligosaccharides equipped with fluorogenic groups for FRET (2009)
  • Author(s): 武田尚子, 大瀧未来, 池田礼美奈, 南條美輪, 田村純一
  • Organizer: XXth International Symposium on Glycoconjugates
  • Place of Presentation: San Juan (Puerto Rico)
  • Year and Date: 2009-12-01
• [Presentation] Synthesis of Primers for Biosynthesis of Proteoglycan (2009)
  • Author(s): J.Tamura, T.Nakamura, A.Imazu
  • Organizer: XXth International Symposium on Glycoconjugates
  • Place of Presentation: San Juan (Puerto Rico)
  • Year and Date: 2009-12-01
• [Presentation] Fam20Bはグリコサミノグリカンの生合成に関わるキシロースリン酸化酵素である (2009)
  • Author(s): 小池敏靖、泉川友美、田村純一,北川裕之
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 高山
  • Year and Date: 2009-09-10
• [Presentation] 酵素活性検出用オリゴ糖鎖の合成 (2009)
  • Author(s): 田村純一、大瀧未来、池田礼美奈、南條美輪、武田尚子
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 高山
  • Year and Date: 2009-09-10
• [Presentation] 保護硫酸基を利用するオリゴ糖鎖の合成 (2009)
  • Author(s): 田村純一、船本真司、松下健也
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 高山
  • Year and Date: 2009-09-10
• [Presentation] 鳥取大学における知的財産動向の分析を踏まえた活用評価システムの検討 (2009)
  • Author(s): 田端海、山岸大輔、清水克彦、田村純一、佐々木茂雄
  • Organizer: 産学連携学会第7回大会
  • Place of Presentation: 福井
  • Year and Date: 2009-08-17
• [Presentation] 植物毒-radicinin-の生合成-第3報 (2009)
  • Author(s): 鈴木大進、松永祥吾、作野えみ、田村純一、中島廣光
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] 魚介類由来コンドロイチン硫酸の含有量と組成分析 (2009)
  • Author(s): 田村純一、石原幸雄、増田紳哉、中山清、川野愛美、田中晴奈、松下健也、藤田裕之
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] 植物毒-radicinin-の生合成-第2報 (2008)
  • Author(s): 鈴木大進、松永祥吾、作野えみ、田村純一、中島廣光
  • Organizer: 日本農芸化学会中四国支部第22回講演会
  • Place of Presentation: 鳥取
  • Year and Date: 2008-09-12
• [Presentation] 魚類由来コンドロイチン硫酸の組成分析 (2008)
  • Author(s): 田村純一、川野愛美、田中晴奈、藤田裕之、松下健也、今津綾夏、堤下奈緒、有馬和也、石原幸雄、中山清
  • Organizer: 日本農芸化学会中四国支部第22回講演会
  • Place of Presentation: 鳥取
  • Year and Date: 2008-09-12
• [Presentation] 4種のコンドロイチン硫酸合成酵素ファミリーの酵素複合体によるコンドロイチン硫酸の合成機構 (2008)
  • Author(s): 小池敏靖、泉川友美、塩澤章子、菅原一幸、田村純一,北川裕之
  • Organizer: 第28回日本糖質学会年会、つくば
  • Year and Date: 2008-08-19
• [Presentation] プロテオグリカン生合成プライマーの合成 (2008)
  • Author(s): 田村純一、仲村友美、今津綾夏
  • Organizer: 第28回日本糖質学会年会、つくば、
  • Year and Date: 2008-08-19
• [Presentation] Identification of Chondroitin Sulfate Glucuronyltransferase as Chondroitin Synthase-3 Involved in Chondroitin Polymerization : CHONDROITIN POLYMERIZATION IS ACHIEVED BY MULTIPLE ENZYME COMPLEXES CONSISTING OF CHONDROITIN SYNTHASE FAMILY MEMBERS (2008)
  • Author(s): 泉川友美, 小池敏靖, 塩澤章子, 菅原一幸, 田村純一, 北川裕之
  • Organizer: XIXth International Carbohydrate Symposium
  • Place of Presentation: Oslo (Norway)
  • Year and Date: 2008-08-02
• [Presentation] Effective Synthesis of the Chondroitin Sulfate E Repeating Oligosaccharide via Acetamide Auxiliary and the Pharmaceutical effect for Collagen-Induced Arthritis in Mice (oral presentation) (2008)
  • Author(s): 田村純一
  • Organizer: XIXth International Carbohydrate Symposium
  • Place of Presentation: Oslo (Norway)
  • Year and Date: 2008-08-02
• [Presentation] Isolation and Characterization of Chondroitin Sulfate from Marine Sources (oral presentation) (2008)
  • Author(s): J.Tamura, K.Nakayama, N.Takeda, Y.Nakao, H.Wang, A.Imazu, N.Tsutsumishita, K.Arima
  • Organizer: XIXth International Carbohydrate Symposium
  • Place of Presentation: Oslo (Norway)
  • Year and Date: 2008-08-02
• [Presentation] プロテオグリカンの化学合成と生物活性 (2008)
  • Author(s): 田村純一
  • Organizer: 糖鎖機能研究会(招待講演)
  • Place of Presentation: 岡崎
  • Year and Date: 2008-05-22
• [Presentation] コンドロイチン合成酵素複合体によるコンドロイチン鎖の合成機構 (2008)
  • Author(s): 泉川友美、小池敏靖、塩澤章子、菅原一幸、田村純一、北川裕之
  • Organizer: 第9回関西グライコサイエンスフォーラム
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-17
• [Book] 複合糖質の化学と最新応用技術(第2編第5章プロテオグリカンの化学合成をめざして)(正田晋一郎、稲津敏行監修) (2009)
  • Author(s): 田村純一, 他
  • Total Pages: 154-160
  • Publisher: シーエムシー出版
• [Book] 地域環境学への招待(5章地域の未利用資源の活用2節水産系不可食部の高付加価値化)(岡田昭明編) (2009)
  • Author(s): 田村純一, 他
  • Total Pages: 95-102
  • Publisher: 三恵社
• [Book] Experimental Glycoscience Glycochemistry(Part 2 Chemical Synthesis of Sugar Chains, Section VI Chemical Synthesis of Sugar Chains Glycosaminoglycans) (2008)
  • Author(s): 田村純一, et. al.
  • Total Pages: 186-190
  • Publisher: Springer