Analysis of calcification mechanism in Haptophyta, using cDNA macroarray

2011 Fiscal Year Final Research Report

• Project/Area Number: 20570059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: FUJIWARA Shoko 東京薬科大学, 生命科学部, 准教授 (30266895)
• Co-Investigator(Kenkyū-buntansha): TSUZUKI Mikio 東京薬科大学, 生命科学部, 教授 (70155430)
• Project Period (FY): 2008 – 2011
• Keywords: 石灰化 / cDNAアレイ / ハプト藻 / 円石藻 / バイオミネラリゼーション

Research Abstract

cDNA macroarray analyses were performed in a pair of coccolith-bearing and naked strains of Pleurochrysis haptonemofera, cultures grown under various conditions to alter calcification rate, and wild type and coccolith-deficient mutants, PS2^-and PS3^-, of P. carterae. The results suggested that genes including those for carbonic anhydrase and keratin-associated protein are involved in coccolith formation.

Research Products

• [Journal Article] Effects of granule-bound starch synthase I-defective mutation on the morphology and structure of pyrenoidal starch in Chlamydomonas (2011)
  • Author(s): Izumo, A., S. Fujiwara, T. Sakurai, S. G. Ball, Y. Ishii, H. Ono, M. Yoshida, N. Fujita, Y. Nakamura, A. Buleon and M. Tsuzuki
  • Journal Title: Plant Sci Volume: 180 Pages: 238-45
• [Journal Article] Rapid biotransformation of arsenate into oxo-arsenosugars by a freshwater unicellular green alga, Chlamydomonas reinhardtii. (2011)
  • Author(s): Miyashita, S., S. Fujiwara, M. Tsuzuki and T. Kaise
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 75 Pages: 522-30
• [Journal Article] The primitive rhodophyte Cyanidioschyzon merolae contains a semiamylopectin-type but not an amylose-type α-glucan. (2010)
  • Author(s): Hirabaru, C., A. Izumo, S. Fujiwara, Y. Tadokoro, T. Shimonaga, M. Konishi, M. Yoshida, N. Fujita, Y. Nakamura, M. Yoshida, T. Kuroiwa and M. Tsuzuki
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 682-93
• [Journal Article] Carbohydrate metabolism in the mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis (2010)
  • Author(s): Suzuki, E., H. Ohkawa, K. Moriya, T. Matsubara, Y. Nagaike, I. Iwasaki, S. Fujiwara, M. Tsuzuki and Y. Nakamura
  • Journal Title: Appl. Environ. Microbiol Volume: 76 Pages: 3153-9
• [Journal Article] Effects of Ca and Mg on growth and calcification of the coccolithophorid Pleurochrysis haptonemofera : Ca-requirement for cell division in coccolith-bearing cells and for normal coccolith-formation with acidic polysaccharides (2010)
  • Author(s): Katagiri, F., Y. Takatsuka, S. Fujiwara and M. Tsuzuki
  • Journal Title: Mar. Biotechnol Volume: 12 Pages: 42-51
• [Journal Article] Structural and physiological studies on the storage β-polyglucan of haptophyte Pleurochrysis haptonemofera (2008)
  • Author(s): Hirokawa, Y., S. Fujiwara, M. Suzuki, T. Akiyama, M. Sakamoto, S. Kobayashi and M. Tsuzuki
  • Journal Title: Planta Volume: 227 Pages: 589-599
• [Journal Article] Variation in storage α-glucans of the Porphyridiales(Rhodophyta) (2008)
  • Author(s): Shimonaga, T., M. Konishi, Y, Oyama, S. Fujiwara, A. Satoh, N. Fujita, C. Colleoni, A. Buleon, J.-L. Putaux, S. G. Ball, A. Yokoyama, Y. Hara, Y. Nakamura and M. Tsuzuki
  • Journal Title: Plant Cell Physiol Volume: 49 Pages: 103-116
• [Presentation] 円石藻Pleurochrysis carteraeにおけるココリス形成の分子生物学的解析 (2011)
  • Author(s): 遠藤博寿、笠島大貴、藤原祥子、都筑幹夫、猿渡和子、小暮敏博、長澤寛道
  • Organizer: 第6回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京
  • Year and Date: 20111200
• [Presentation] 円石藻のココリスの有機基板に含まれるタンパク質の同定と発現解析 (2010)
  • Author(s): 笠島大貴、遠藤博寿、長坂征治、藤原祥子、都筑幹夫、長澤寛道
  • Organizer: 第5回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京
  • Year and Date: 20101100
• [Presentation] 海産性植物プランクトン、円石藻の石灰化(ココリス形成) (2009)
  • Author(s): 藤原祥子
  • Organizer: 第4回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京
  • Year and Date: 20091200
• [Presentation] 円石藻Pleurochrysisの石灰化における2価金属イオンと重炭酸イオンの影響 (2009)
  • Author(s): 高塚由紀子、藤原祥子、片桐史朗、須田邦裕、柴田大輔、田畑哲之、都筑幹夫
  • Organizer: 第3回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京
  • Year and Date: 20091200
• [Presentation] 円石藻Pleurochrysis haptonemoferaの円石形成関連遺伝子の網羅的解析と環境因子の影響 (2009)
  • Author(s): 高塚由紀子、藤原祥子、須田邦裕、中島大輔、岡崎孝映、柴田大輔、田畑哲之、都筑幹夫
  • Organizer: 第12回マリンバイオテクノロジー学会
  • Place of Presentation: 東京
  • Year and Date: 20090500
• [Presentation] 円石藻Pleurochrysis haptonemoferaの石灰化に及ぼす環境因子の影響 (2009)
  • Author(s): 高塚由紀子、藤原祥子、片桐史朗、柴田大輔、田畑哲之、都筑幹夫
  • Organizer: 日本藻類学会第33回大会
  • Place of Presentation: 那覇
  • Year and Date: 20090300
• [Presentation] マクロアレイを用いた円石藻の円石形成細胞特異的発現遺伝子の網羅的解析 (2008)
  • Author(s): 藤原祥子、広川安孝、高塚由紀子、須田邦裕、浅水恵理香、高柳賢利、柴田大輔、田畑哲之、都筑幹夫
  • Organizer: 第11回マリンバイオテクノロジー学会大会
  • Place of Presentation: 京都
  • Year and Date: 20080500
• [Book] Coccolith formation in the Haptophyta. In Marine Phytoplankton (2009)
  • Author(s): Fujiwara, S. and M. Tsuzuki
  • Publisher: Nova Science Publishers, Inc
• [Book] Arsenic metabolism in cyanobacteria. In Cyanobacteria : Toxicity, Ecology and Management
  • Author(s): Miyashita S., K. Kondo, M. Tsuzuki and S. Fujiwara
  • Publisher: Nova Science Publishers, Inc.(in press)
• [Book] 藻類ハンドブック(渡邊信監修)、第2章代謝と物質、第1節代謝と物質、5糖類-デンプン他
  • Author(s): 藤原祥子、都筑幹夫
  • Publisher: 株式会社エヌ・ティー・エス(in press)