2010 Fiscal Year Final Research Report
Analysis of yeast Cch1, a homologue of animal voltage-gated calcium channel pore subunit, and its regulatory factor Mid1.
| Project/Area Number |
20570158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
IIDA Kazuko Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (40151229)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Hidetoshi 東京学芸大学, 教育学部, 教授 (70124435)
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| Research Collaborator |
TENG Jinfeng 群馬大学, 医学系研究科, 博士課程院生
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| Project Period (FY) |
2008 – 2010
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| Keywords | 生体膜 / 受容体 / カルシウムチャネル |
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| Research Abstract |
Cch1 is a yeast homologue of the pore-forming α1 subunit of animal voltage-gated calcium channels (VGCC), and Mid1 is an essential component together with Cch1 for the high-affinity calcium uptake in yeast. We tried to detect electric currents through the Cch1/Mid1 channel expressed in cultured mammalian cells or in yeast spheroplasts by the patch-clump method. Mid1 is heavily N-glycosylated allover the length, and Mid1 has a cysteine-rich motif in the C-terminal region. These characteristics suggest that Mid1 is a functional homologue of the α_2/δsubunit of animal VGCCs.
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[Journal Article] MCA1 and MCA2 that mediate Ca^<2+> uptake have distinct and overlapping roles in Arabitopsis thaliana.2010
Author(s)
Yamanaka T., Nakagawa Y., Mori K., Nakano M., Imamura T., Kataoka H., Terashima A., Iida K., Kojima I., Katagiri T., Shinozaki K., Iida H.
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Journal Title
Plant Physiol. 152
Pages: 1284-1296
Peer Reviewed
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