2010 Fiscal Year Final Research Report

Elucidation of genetic mechanisms that generate structural diversity of a group of sesquiterpenes involved in plant-microbe interactions

Research Project

Project/Area Number	20580117
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Bioproduction chemistry/Bioorganic chemistry
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	KIMURA Makoto 独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員 (20261167)
Research Collaborator	富永直樹東京理科大学, 理工学部, 修士前田一行明治大学, 農学部・農学科, 博士
Project Period (FY)	2008 – 2010
Keywords	Biosynthesis / Deacetylase / Fusarium graminearum / Nivalenol / Trichothecenes
Research Abstract	Fusarium graminearum, a head blight fungus that causes devastating disease of wheat and other important cereal crops, produces trichothecene mycotoxins, a diverse group of sesquiterpene secondary metabolites. They are also known to act as a virulence factor of this pathogen. In an attempt to elucidate the mechanisms that generate the diversity of side chains, we have identified and characterized a C-4 deaetylase gene, Tri104, outside of the gene trichothecene cluster. Tri104 was expressed when the fungus was grown on solid rice(SR), but not on liquid rice flour(RF) medium. This result coincides with the fact that the fungus produces nivalenol, a trichothecene with a hydroxyl at C-4, only on SR(but not on RF) medium.

(1 results)