Molecular basis of the rhythmical expression of the genes in the gastrointestinal tract

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: University of Shizuoka
• Principal Investigator: GODA Toshinao University of Shizuoka, 食品栄養科学部, 教授 (70195923)
• Co-Investigator(Kenkyū-buntansha): MOCHIZUKI Kazuki 静岡県立大学, 食品栄養科学部, 助教 (80423838)
• Project Period (FY): 2008 – 2010
• Keywords: 時計遺伝子 / 小腸 / 日内リズム / 転写調節 / マイクロアレイ

Research Abstract

Restricted feeding schedule led to a shift of the peak of the expression of clock gene (per 2) and a hexose transporter gene (sglt1) to just prior to the start of feeding. The binding of BMAL1 and acetylated histone H3 and H4 to the transcribed regions of these genes was associated with changes in their expressions. These results suggest that diurnal changes in expression of hexose transporter genes are regulated by a feedback loop of clock genes.

Research Products

• [Journal Article] Clock genes regulate the feeding schedule-dependent diurnal rhythm changes in hexose transporter gene expressions through the binding of BMAL1 to the promoter/enhancer and transcribed regions. (2011)
  • Author(s): Iwashina, I., Mochizuki, K., Inamochi, Y., Goda, T.
  • Journal Title: J.Nutr.Biochem. Vol.22 Pages: 334-343
• [Journal Article] Histone H3 methylation at lysine 4 on the SLC2A5 gene in intestinal Caco-2 cells is involved in SLC2A5 expression. (2010)
  • Author(s): Inamochi, Y., Mochizuki, K., Osaki, A., Ishii, T., Nakayama, T., Goda, T.
  • Journal Title: Biochem.Biophys.Res.Commu. Vol.392 Pages: 16-21
• [Journal Article] Inductions of histone H3 acetylation at lysine 9 on SGLT1 gene and its expression by feeding mice a high carbohydrate/fat ratio diet. (2008)
  • Author(s): Honma, K., Mochizuki, K., Goda, T.
  • Journal Title: Nutrition Vol.25 Pages: 40-44
• [Presentation] ヒストンコードとmRNA伸長因子を介した小腸消化吸収関連遺伝子発現の日内リズム発振機構 (2010)
  • Author(s): 山内浩之、望月和樹、合田敏尚
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: 徳島
  • Year and Date: 2010-05-23
• [Presentation] マウス脂肪組織におけるヒストンコードを介した脂肪合成関連遺伝子発現の日内リズム (2009)
  • Author(s): 五十嵐信人、望月和樹、合田敏尚
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Clock genes regulate circadian rhythmical change of sglt1 gene expression according to feeding schedule through binding of BMAL1 on the promoter region. (2009)
  • Author(s): Goda, T., Iwashina, I., Mochizuki K.
  • Organizer: Experimental Biology 2009
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-04-20
• [Book] 臨床栄養医学 (2009)
  • Author(s): 合田敏尚
  • Total Pages: 96-101
  • Publisher: 南山堂