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2010 Fiscal Year Annual Research Report

尿路病原性大腸菌が産生するUSPの作用解析と粘膜ワクチンの開発

Research Project

Project/Area Number 20590436
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

倉園 久生  国立大学法人帯広畜産大学, 畜産学部, 教授 (90186487)

Co-Investigator(Kenkyū-buntansha) 山本 新吾  兵庫医科大学, 医学部, 教授 (80322741)
山崎 栄樹  国立大学法人 帯広畜産大学, 畜産学部, 助教 (40514708)
Keywords細菌 / 尿路感染症 / 蛋白質 / 免疫学 / 粘膜
Research Abstract

尿路病原性大腸菌(UPEC)が産生するUSPは、1)それ自体で尿路感染を惹起し、2)Immunity proteinと推定される蛋白と共に発現されて周囲の細菌を殺してその菌がdominantになり、3)トランスポゾン様に周囲の大腸菌(あるいは他種の細菌にも)に伝搬され、4)分子疫学的にもUPECに局在し、5)愛玩動物の尿路感染症由来株のUPECもヒトと全く同様の性状および挙動を示す。これらの研究結果と他の細菌性疾患同様、尿路感染症で分離されるUPECも薬剤耐性菌が増加の一途をたどっている事により、UPECが特異的に分泌していると考えられるUSPに対する粘膜ワクチンを開発することで、UPECが保持されている腸管並びに感染部位である尿路系でのUPECの増殖を抑制してUPECによる尿路感染症を減少させる事を目的とする。
昨年度、1アミノ酸置換無毒化USP融合蛋白(mUSP-GST)を高純度に精製して抗mUSP-GST家兎血清を調整し、この抗血清を更にmUSP-GSTを結合させたAffinityカラムで精製してmUSP-GST特異IgGを調整した。この特異IgGを用いたSandwich-ELISA系を構築して精製mUSP-GSTに対する検出感度を検討したところ、検出限界は500pg/mlであった。このELISA系を用いて臨床分離UPEC株のUSP産生量を調べたところ感度不足により検出できなかった。このため、本年度は特異IgGからFab'を調整して更に感度の高いBead-ELISA系を構築した。このELISA系の検出感度は100pg/mlで5倍も感度を上げることができた。現在、この検出系を用いてUPEC臨床分離株のUSP産生量の調査を行っている。

  • Research Products

    (20 results)

All 2011 2010

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (8 results)

  • [Journal Article] Helicobacter pylori VacA reduces cellular expression of STAT3 and pro-survival Bcl-2 family proteins, Bcl-2 and Bcl-X_L, leading to apoptosis in gastric epithelial cells2011

    • Author(s)
      Matsumoto A, Isomoto H, Nakayama M, Hisatsune J, Nishi Y, Nakashima Y, Matsushima K, Kurazono H, Nakao K, Hirayama T, Kohno S
    • Journal Title

      Digestive Diseases and Science

      Volume: 56 Pages: 999-1006

    • Peer Reviewed
  • [Journal Article] Hybrid & EI Tor variant biotypes of Vibrio cholerae O1 in Thailand2011

    • Author(s)
      Na-Ubol, M, Srimanote P, Chongsa-nguan M, Indrawattana N, Sookrung N, Tapchaisri P, Yamazaki S, Bodhidatta L, Eampokalap B, Kurazono H, Hayashi H, Nair GB, Takeda Y, Chaicumpa W
    • Journal Title

      Indian Journal of Medical Research

      Volume: 133 Pages: 387-394

    • Peer Reviewed
  • [Journal Article] A single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urological clean and clean-contaminated surgery2011

    • Author(s)
      Higuchi Y, Takesue Y, Yamada Y, Ueda Y, Suzuki T, Aihara K, Mane yama T, Kondoh N, Nojima M, Yamamoto S
    • Journal Title

      Journal of Infection and Chemotheraphy

      Volume: 17 Pages: 219-222

    • Peer Reviewed
  • [Journal Article] Double short-time exposure to pirarubicin produces higher cytotoxity against T24 bladder cancer cells2011

    • Author(s)
      Maruyama T, Higuchi Y, Suzuki T, Qiu J, Yamamoto S, Shima H
    • Journal Title

      Journal of Infection and Chemotheraphy

      Volume: 17 Pages: 11-16

    • Peer Reviewed
  • [Journal Article] The nationwide study of bacterial pathogens associated with urinary tract infections conducted by the Japanese Society of Chemotherapy2011

    • Author(s)
      Ishikawa K, Matsumoto T, Yasuda M, Uehara S, Muratani T, Yagisawa M, Sato J, Niki Y, Totsuka K, Sunakawa K, Hanaki H, Hattori R, Terada M, Kozuki T, Maruo A, Morita K, Ogasawara K, Takahashi Y, Matsuda K, Hirose T, Miyao N, Hayashi T, Takeyama K, Kiyota H, Tomita M, Yusu H, Koide H, Kimura S, Yanaoka M, Sato H, Ito T, Deguchi T, Fujimoto Y, Komeda H, Asano Y, Takahashi Y, Ishihara S, Arakawa S, Nakano Y, Tanaka K, Fujisawa M, Matsui T, Fujii A, Yamamoto S, Nojima M, Higuchi Y, Ueda Y, Kanamaru S, Monden K, Tsushima T, Seno Y, Tsugawa M, Takenaka T, Hamasuna R, Fujimoto N, Sho T, Takahashi K, Inatomi H, Takahashi N, Ikei Y, Hayami H, Yamane T, Nakagawa M, Kariya S, Arima T
    • Journal Title

      Journal of Infection and Chemotheraphy

      Volume: 17 Pages: 126-138

    • Peer Reviewed
  • [Journal Article] A V_HH that neutralizes the zinc-metalloproteinase activity of botulinum neurotoxin type A2010

    • Author(s)
      Thanongsaksrikul J, Srimanote P, Maneewatch S, Choowongkomon K, Tapchaisri P, Makino S-I, Kurazono H, Chaicumpa W
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 9657-9666

    • Peer Reviewed
  • [Journal Article] Helicobacter pylori CagA Inhibits Endocytosis of Cytotoxin VacA in Host Cells2010

    • Author(s)
      Akada KJ, Aoki H, Torigoe Y, Kitagawa T, Kurazono H, Hoshida H, Nishikawa J, Terai S, Matsuzaki M, Hirayama T, Nakazawa T, Akada R, Nakamura K
    • Journal Title

      Disease Models & Mechanisms

      Volume: 3 Pages: 605-617

    • Peer Reviewed
  • [Journal Article] Far-infrared rays control prostate cancer cells in vitro and in vivo2010

    • Author(s)
      Shima H, Yamamoto S, Qiu J, Shin cho M, Hirota S, Yoshikawa Y, Yoshikawa R, Hashimoto-Tamaoka T
    • Journal Title

      Central European Journal of Biology

      Volume: 5 Pages: 178-189

    • Peer Reviewed
  • [Journal Article] Prognostic of Japanese metastatic renal cell carcinoma (mRCC) patients in the cytokine era-a cooperative group report of 1,463 mRCC patients2010

    • Author(s)
      Naito S, Yamamoto N, Takayama T, Muramoto M, Shinohara N, Nishiyama K, Takahashi A, Maruyama R, Saika T, Hoshi S, Nagao K, Yamamoto S, Sugimura I, Umemura H, Koga S, Takahashi M, Ito F, Ozono S, Terachi T, Naito S, Tomita Y
    • Journal Title

      European Journal of Urology

      Volume: 57 Pages: 317-325

    • Peer Reviewed
  • [Journal Article] A transient increase of calcineurin phosphatase activity in living-donor kidney transplant recipient with acute rejection2010

    • Author(s)
      Fukudo M, Yano I, Katsuya T, Ito N, Yamamoto S, Kamoto T, Ogawa O, Inui K
    • Journal Title

      Drug Metab pharmacokinet

      Volume: 25 Pages: 411-417

    • Peer Reviewed
  • [Journal Article] The antitumour effect of γ δ T Cells is enhanced by valproic acid-induced up-regulation of NKG2D ligands2010

    • Author(s)
      Suzuki T, Terao S, Acharya B, Naoe M, Yamamoto S, Okamura H, Gotoh A
    • Journal Title

      Anticancer Research

      Volume: 30 Pages: 4509-4514

    • Peer Reviewed
  • [Journal Article] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity2010

    • Author(s)
      Wada A, Hasegawa M, Wong P F, Shirai E, Shirai N, Tan L J, Llanes R, Hojo H., Yamasaki E, Ichinose A, Ichinose Y, Senba M
    • Journal Title

      Glycobiology

      Volume: 20 Pages: 668-678

    • Peer Reviewed
  • [Presentation] A randomized clinical trial to evaluate the preventive effect of cranberry juice(UR65)for patients with recurrent urinary tract infection2011

    • Author(s)
      Takahashi S, Hamasuna R, Yasuda M, Arakawa S, Tanaka K, Ishikawa K, Kiyota H, Hayami H, Yamamoto S, Matsumoto T
    • Organizer
      IICC/ECCMID 2011
    • Place of Presentation
      Milan, Italy
    • Year and Date
      2011-05-07
  • [Presentation] Analysis of Pthogenicity island of Uropathogenic Escherichia coli which encodes the USP2010

    • Author(s)
      Kurazono H
    • Organizer
      SPU Forum : Updates on Common Viral and Bacterial Infections
    • Place of Presentation
      Universiti Malaysia Sabah, Malaysia
    • Year and Date
      2010-10-25
  • [Presentation] Education for Sustainable Development (ESD) on Infectious Diseases and Global Environmental Issues2010

    • Author(s)
      Smith M, Kurazono H
    • Organizer
      14^<th> UNESCO-APEID International Conference Education for Human Resource Development
    • Place of Presentation
      Imperial Queen's Park Hotel, Bangkok, Thailand
    • Year and Date
      2010-10-23
  • [Presentation] Optimal treatment with antibiotics : single dose, short course(3 days), or standard course(7 days)therapy2010

    • Author(s)
      Yamamoto S
    • Organizer
      10th Asian Congress of Urology, Urological Association of Asia
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2010-08-29
  • [Presentation] Assessment of a single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urologic surgery2010

    • Author(s)
      Yamamoto S
    • Organizer
      German-Japanese Confederation of Urology 9th Scientific Meeting
    • Place of Presentation
      Munster, Germany
    • Year and Date
      2010-06-19
  • [Presentation] Enhancement of γ δ T cell antitumor activity against prostate cancer cell through Toll-like receptor 2 and 3 ligand dependent activation2010

    • Author(s)
      Suzuki T, Tacharya B, Terao S, Nojima M, Yamamoto S, Gotoh A
    • Organizer
      AUA Annual Meeting'10
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2010-05-29
  • [Presentation] Assessment of a single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urologic surgery2010

    • Author(s)
      Higuchi Y, Yamada Y, Ueda Y, Suzuki Y, Aihara K, Maruyama T, Noiima M, Yamamoto S
    • Organizer
      AUA Annual Meeting'10
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2010-05-29
  • [Presentation] Antimicrobial Prophylaxis in Urological Surgery2010

    • Author(s)
      Yamamoto S
    • Organizer
      8^<th> Annual Congress of the KAUTII
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2010-05-01

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Published: 2012-07-19  

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