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2010 Fiscal Year Final Research Report

The role of CM2 ion channel protein in influenza C virus replication and pathogenesis

Research Project

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Project/Area Number 20590465
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionYamagata University

Principal Investigator

HONGO Seiji  Yamagata University, 医学部, 教授 (90229245)

Co-Investigator(Kenkyū-buntansha) MURAKI Yasushi  金沢医科大学, 医学部, 准教授 (00241688)
Project Period (FY) 2008 – 2010
KeywordsC型インフルエンザウイルス / イオンチャネル / CM2 / 増殖 / 病原性
Research Abstract

Quantification of vRNA in the VLPs by real-time PCR revealed that the CM2-deficient VLPs contain approximately one-third of the vRNA found in wild-type VLPs, suggesting that CM2 is involved in the genome packaging process into VLPs. Furthermore, a smaller amount of vRNA was detected in the nuclear fraction of CM2-deficient VLP-infected cells than in that of wild-type VLP-infected cells, suggesting that the uncoating process of the CM2-deficient VLPs in the infected cells did not proceed in an appropriate manner. Taken together, it is suggested that CM2 has a potential role in the genome packaging and uncoating processes of the virus replication.

  • Research Products

    (23 results)

All 2011 2010 2009 2008

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (14 results)

  • [Journal Article] Palmitoylation of CM2 is dispensable to influenza C virus replication.2011

    • Author(s)
      Muraki Y, Okuwa T, Furukawa T, Matsuzaki Y, Sugawara K, Himeda T, Hongo S, Ohara Y
    • Journal Title

      Virus Res. 157(1)

      Pages: 99-105

    • Peer Reviewed
  • [Journal Article] Role of the CM2 protein in the influenza C virus replication cycle.2011

    • Author(s)
      Furukawa T, Muraki Y, Noda T, Takashita E, Sho R, Sugawara K, Matsuzaki Y, Shimotai Y, Hongo S
    • Journal Title

      J Virol. 85(3)

      Pages: 1322-9

    • Peer Reviewed
  • [Journal Article] The molecular virology and reverse genetics of influenza C virus.2010

    • Author(s)
      Muraki Y, Hongo S
    • Journal Title

      Jpn J Infect Dis. 63(3)

      Pages: 157-65

    • Peer Reviewed
  • [Journal Article] Influenza C virus NS1 protein upregulates the splicing of viral mRNAs.2010

    • Author(s)
      Muraki Y, Furukawa T, Kohno Y, Matsuzaki Y, Takashita E, Sugawara K, Hongo S
    • Journal Title

      J Virol. 84(4)

      Pages: 1957-66

    • Peer Reviewed
  • [Journal Article] Development and evaluation of a whole virus-based enzyme-linked immunosorbent assay for the detection of human metapneumovirus antibodies in human sera.2010

    • Author(s)
      Okamoto M, Sugawara K, Takashita E, Muraki Y, Hongo S, Mizuta K, Itagaki T, Nishimura H, Matsuzaki Y
    • Journal Title

      J Virol Methods. 164(1-2)

      Pages: 24-9

    • Peer Reviewed
  • [Journal Article] Longitudinal course of human metapneumovirus antibody titers and reinfection in healthy adults.2010

    • Author(s)
      Okamoto M, Sugawara K, Takashita E, Muraki Y, Hongo S, Nishimura H, Matsuzaki Y
    • Journal Title

      J Med Virol. 82(12)

      Pages: 2092-6

    • Peer Reviewed
  • [Journal Article] Cross-antigenicity among EV71 strains from different genogroups isolated in Yamagata, Japan, between 1990 and 2007.2009

    • Author(s)
      Mizuta K, Aoki Y, Suto A, Ootani K, Katsushima N, Itagaki T, Ohmi A, Okamoto M, Nishimura H, Matsuzaki Y, Hongo S, Sugawara K, Shimizu H, Ahiko T
    • Journal Title

      Vaccine. 27(24)

      Pages: 3153-8

    • Peer Reviewed
  • [Journal Article] Intracellular localization of influenza C virus NS2 protein (NEP) in infected cells and its2009

    • Author(s)
      Kohno Y, Muraki Y, Matsuzaki Y, Takashita E, Sugawara K, Hongo S
    • Journal Title

      incorporation into virions. Arch Virol. 154(2)

      Pages: 235-43

    • Peer Reviewed
  • [Journal Article] Stability of the seven hexon hypervariable region sequences of adenovirus types 1-6 isolated in Yamagata, Japan between 1988 and 2007.2009

    • Author(s)
      Mizuta K, Matsuzaki Y, Hongo S, Ohmi A, Okamoto M, Nishimura H, Itagaki T, Katsushima N, Oshitani H, Suzuki A, Furuse Y, Noda M, Kimura H, Ahiko T
    • Journal Title

      Virus Res. 140(1-2)

      Pages: 32-9

    • Peer Reviewed
  • [Presentation] Influenza C virus NS1 protein up-regulates viral mRNA splicing.2010

    • Author(s)
      Hongo S
    • Organizer
      Options for the Control of Influenza VII
    • Place of Presentation
      HongKong SAR, China
    • Year and Date
      20100903-20100907
  • [Presentation] The role of the CM2 protein in the influenza C virus replication.2010

    • Author(s)
      Furukawa T
    • Organizer
      Options for the Control of Influenza VII
    • Place of Presentation
      HongKong SAR, China
    • Year and Date
      20100903-20100907
  • [Presentation] Role of palmitoylation of CM2 in the influenza C virus replication.2010

    • Author(s)
      Muraki Y
    • Organizer
      Options for the Control of Influenza VII
    • Place of Presentation
      HongKong SAR, China
    • Year and Date
      20100903-20100907
  • [Presentation] Overexpression of UBE2L6 gene inhibits the propagation of influenza virus.2010

    • Author(s)
      Shimotai Y
    • Organizer
      Options for the Control of Influenza VII
    • Place of Presentation
      HongKong SAR, China
    • Year and Date
      20100903-20100907
  • [Presentation] The role of the influenza C virus CM2 protein in virus replication.2009

    • Author(s)
      Furukawa T
    • Organizer
      山形大学グローバルCOE平成21年度国際シンポジウム
    • Place of Presentation
      山形
    • Year and Date
      2009-11-06
  • [Presentation] C型インフルエンザウイルスの増殖過程におけるCM2蛋白の役割2009

    • Author(s)
      古川孝俊
    • Organizer
      第57回日本ウイルス学会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-26
  • [Presentation] C型インフルエンザウイルスのCM2蛋白のアシル化の意義2009

    • Author(s)
      村木靖
    • Organizer
      第57回日本ウイルス学会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-25
  • [Presentation] C型インフルエンザウイルスのCM2蛋白のアシル化の意義2009

    • Author(s)
      村木靖
    • Organizer
      第62回日本細菌学会東北支部総会
    • Place of Presentation
      盛岡
    • Year and Date
      2009-08-21
  • [Presentation] C型インフルエンザウイルスの増殖過程におけるCM2蛋白の役割の解析2009

    • Author(s)
      古川孝俊
    • Organizer
      第62回日本細菌学会東北支部総会
    • Place of Presentation
      盛岡
    • Year and Date
      2009-08-21
  • [Presentation] C型インフルエンザウイルスの粒子形成機構に関する一考察2009

    • Author(s)
      村木靖
    • Organizer
      第23回インフルエンザ研究者交流の会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2009-07-03
  • [Presentation] C型インフルエンザウイルスの増殖におけるCM2蛋白の役割2009

    • Author(s)
      古川孝俊
    • Organizer
      第23回インフルエンザ研究者交流の会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2009-07-03
  • [Presentation] C型インフルエンザウイルスの転写後調節2008

    • Author(s)
      本郷誠治
    • Organizer
      第56回日本ウイルス学会学術集会
    • Place of Presentation
      岡山市
    • Year and Date
      2008-10-26
  • [Presentation] C型インフルエンザウイルスNS1蛋白のスプライシング調節能2008

    • Author(s)
      本郷誠治
    • Organizer
      第62回日本細菌学会東北支部総会
    • Place of Presentation
      十和田市
    • Year and Date
      2008-08-21
  • [Presentation] C型インフルエンザウイルスの増殖過程におけるCM2蛋白の役割の解析2008

    • Author(s)
      古川孝俊
    • Organizer
      第62回日本細菌学会東北支部総会
    • Place of Presentation
      十和田市
    • Year and Date
      2008-08-21

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Published: 2012-01-26   Modified: 2016-04-21  

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