Molecular Mechanisms of Establishment, Maintenance and Breakdown of Viral Persistent Infection

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590478
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Chubu University
• Principal Investigator: ITO Yasuhiko Chubu University, 生命健康科学部, 教授 (00022872)
• Co-Investigator(Kenkyū-buntansha): ITO Morihiro 中部大学, 生命健康科学部, 准教授 (10281081)
• Project Period (FY): 2008 – 2010
• Keywords: パラミクソウイルス / 持続感染 / センダイウイルス / 細胞傷害 / インターフェロン

Research Abstract

We tried to devise a quantitative method for analyzing establishing-efficiency of persistent viral infection. Subsequently, we analyzed factors of Sendai virus contributing to persistent infection by applying the quantitative method, indicating that L protein plays a determinative role in establishment of and M protein has an additional ability to induce persistent infection Fpi protein also contributes to persistent viral infection, but HNpi protein shows suppressive effect on establishment of persistent viral infection. HPIV2 induced cell toxicity, that is, giant cell formation, was suppressed by Rho kinase inhibitor, indicating that Rho kinase plays a positive role in HPIV2 induced cell toxicity and C kinase inhibitor enhanced HPIV2 induced cell toxicity. Intriguingly, the protein kinases showed intracellular networks.

Research Products

• [Journal Article] Identification of domains on the fusion (F) protein trimer that influence the hemagglutinin-neuraminidase specificity of the f protein in mediating cell-cell fusion. (2011)
  • Author(s): Tsurudome M, Ito M, Nishio M, Nakahashi M, Kawano M, Komada H, Nosaka T, Ito Y.
  • Journal Title: J Virol. 85(7) Pages: 3153-3161
  • Peer Reviewed
• [Journal Article] Completion of the full-length genome sequence of human parainfluenza virus types 4A and 4B : sequence analysis of the large protein genes and gene start, intergenic and end sequences. (2010)
  • Author(s): Komada H, Kawano M, Uefuji A, Ito M, Tsurudome M, Hatakeyama E, Nakanishi M, Sakue S, Joh C, Suzumura E, Tamaki T, Tomioka T, Nishio M, Tsumura H, Uematsu J, Yamamoto H, O'Brien M, Bando H, Ito Y
  • Journal Title: Arch Virol. 56(1) Pages: 161-166
  • Peer Reviewed
• [Journal Article] Spontaneous Formation of Multinucleated Giant Cells with Bone Resorbing Activity by Long Culture of Human Peripheral Blood CD14-positive Monocytes in vitro (2010)
  • Author(s): Yuasa K, Avnet S, Salerno M, Mori K, Ishikawa H, Sudo A, Baldini N, Uchida A, Ito Y
  • Journal Title: Cell Communication & Adhesion 17(1) Pages: 13-22
  • Peer Reviewed
• [Journal Article] Analysis of N-glycans in embryonated chicken egg chorioallantoic and amniotic cells responsible for binding and adaptation of human and avian influenza viruses. (2009)
  • Author(s): Sriwilaijaroen N, Kondo S, Yagi H, Wilairat P, Hiramatsu H, Ito M, Ito Y, Kato K, Suzuki Y.
  • Journal Title: Glycoconj J. 26(4) Pages: 433-443
  • Peer Reviewed
• [Journal Article] Effects of multiple amino acids of the parainfluenza virus 5 fusion protein on its haemagglutinin-neuraminidase-independent fusion activity. (2009)
  • Author(s): Ito M, Nishio M, Kawano M, Komada H, Ito Y, Tsurudome M.
  • Journal Title: J Gen Virol. 90(Pt 2) Pages: 405-413
  • Peer Reviewed
• [Journal Article] A quantitative method for analyzing establishing-efficiency of persistent viral infection. (2009)
  • Author(s): Ito M, Yamakawa I, Nishio M, Tsurudome M, Kawano M, Komada H, Ito Y.
  • Journal Title: Microbiol Immunol. 53(5) Pages: 259-265
  • Peer Reviewed
• [Journal Article] Effects of hemagglutinin-neuraminidase protein mutations on cell-cell fusion mediated by human parainfluenza type 2 virus (2008)
  • Author(s): Tsurudome M, Nishio M, Ito M, Tanahashi S, Kawano M, Komada H, Ito Y
  • Journal Title: J Virol. 82(17) Pages: 8283-8295
  • Peer Reviewed