2010 Fiscal Year Final Research Report
Development of highly sensitive simultaneous bio- and chemi-luminescent enzyme immunoassay for periodontal pathogens genes
| Project/Area Number |
20590584
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Showa University |
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Principal Investigator |
ITO Katsutoshi Showa University, 薬学部, 准教授 (20223141)
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| Project Period (FY) |
2008 – 2010
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| Keywords | イクオリン / ルシフェラーゼ / ペルオキシダーゼ / 生物発光 / 化学発光 / 同時検出 / イムノアッセイ / 歯周病菌遺伝子 |
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| Research Abstract |
We have developed simultaneous luminometric assay for aequorin, firefly luciferase and horseradish peroxidase. In this study, we applied this analysis method to the detection of the polymerase chain reaction (PCR) products. We selected the periodontal pathogens genes as a measurement genes. Among putative periodontal pathogens, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Treponema denticola are related most as etiological agents in periodontal disease. Therefore, detection of those three pathogens are useful to diagnosis for periodontal disease. We developed simultaneous detection of multiplex PCR products of three periodontal pathogens by using the bio- and chemi-luminescent enzyme immunoassay (BCLEIA). The simultaneous BCLEIA could be highly sensitive detection compared with conventional agarose gel electrophoresis.
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Research Products
(9 results)
