Proteomic investigation of oxidized proteins in cerebrospinal fluid for developing novel biomarkers of Alzheimer's disease.

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20590594
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology (2009-2012); Tokyo Metropolitan Institute of Gerontology (2008)
• Principal Investigator: TODA Tosifusa Tokyo Metropolitan Institute of Gerontology, 東京都健康長寿医療センター研究所, 研究副部長 (80133635)
• Project Period (FY): 2008 – 2012
• Keywords: 臨床検査医学

Research Abstract

(1)二次元電気泳動および質量分析に基づく新たな酸化蛋白質のプロテオーム解析技術を開発し、臨床検査に適用可能なヒト髄液中の酸化修飾蛋白質の定量的分析技術を確立する。
(2)新たに開発された酸化蛋白質のプロテオーム解析技術を用いてアルツハイマー病患者および軽度認知障害(MCI)患者の髄液中の酸化蛋白質を多角的に分析し、早期診断マーカーの候補となる蛋白質を絞り込む。

Research Products

• [Journal Article] Proteomic analysis of plasma proteins in Japanese semisuper centenarians (2011)
  • Author(s): Miura Y, Sato Y, Arai Y, Abe Y, Takayama M, Toda T, Hirose N, Endo T.
  • Journal Title: Experimental Gerontology. 46 Pages: 81-85
  • Peer Reviewed
• [Journal Article] Proteomic approaches to oxidative protein modifications implicated in the mechanism of aging (2010)
  • Author(s): Toda T, Nakamura M, Morisawa H, Hirota M, Nishigaki R, Yoshimi Y.
  • Journal Title: Geriatrics Gerontology International 10(Supplement 1) Pages: S25-S31
  • Peer Reviewed
• [Journal Article] Proteomic analysis of protein complexes in human SH-SY5Y neuroblastoma cells by using blue-native gel electrophoresis : An increase in lamin A/C associated with heat shock protein 90 in response to 6-hydroxydopamine-induced oxidative stress (2009)
  • Author(s): Nakamura M, Morisawa H, Imajoh-Ohmi S, Takamura C, Fukuda H, Toda T
  • Journal Title: Experimental.Gerontology 44(6-7) Pages: 375-382
  • Peer Reviewed
• [Presentation] 脳脊髄液タンパク質の酸化還元状態の多角的プロテオーム解析 (2010)
  • Author(s): 戸田年総、岩本真知子、森澤 拓、吉澤利弘、芝崎 太
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会連合大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] 酸化ストレスによるラミンのリン酸化と分子シャペロン複合体形成のプロテオーム解析 (2010)
  • Author(s): 戸田年総、中村 愛
  • Organizer: 日本電気泳動学会第62回総会
  • Place of Presentation: 札幌
  • Year and Date: 20100918-19
• [Presentation] Proteome Analysis of Carbonyl Proteins in Cerebrospinal Fluid by Cy5-Hydrazide Labeling (2009)
  • Author(s): Tosifusa Toda, Megumi Nakamura, Futoshi shibasaki, Toshihiro Yoshizawa
  • Organizer: HUPO 8th Annual World Congress
  • Place of Presentation: Tronto、Canada
  • Year and Date: 20090926-30
• [Presentation] 二次元目にTris-Tricine緩衝液系SDS-電気泳動を用いた場合のスタッキング効果に影響を及ぼす緩衝液条件の検討:ミニゲル二次元電気泳動のための泳動条件の最適化 (2009)
  • Author(s): 戸田年総, 中村愛, 廣田三佳子, 森澤拓
  • Organizer: 第60回日本電気泳動学会総会
  • Place of Presentation: 松本
  • Year and Date: 20090919-20090920
• [Presentation] Tokyo Metropolitan Geriatric Hospital Autopsy Resource (TMGH-AR) (2009)
  • Author(s): Motoji Sawabe, Tomio Arai, Yuko Saito, Takahiko Shimizu, Tosifusa Toda, Shigeo Murayama, Masashi Tonaka, Kaiyo Takubo, Makiko Naka, Noriko Tanaka
  • Organizer: 19th IAGG World Congress of Gerontology and Geriatrics
  • Place of Presentation: Paris、France
  • Year and Date: 20090705-20090709
• [Presentation] シアニン系蛍光標識試薬を用いたタンパク質フリーチオールレベルの定量分析法に関する基礎的検討 (2008)
  • Author(s): 戸田年総, 中村愛, 森澤拓, 岩本真知子
  • Organizer: 第59回日本電気泳動学会総会
  • Place of Presentation: 東京
  • Year and Date: 20081115-20081116
• [Presentation] An approach to the quantitative analysis of free thiol levels in human CSF proteins by the method of 2-D gel fluoroimaging with using cyanine dyes and fluorescent gel stain (2008)
  • Author(s): Tosifusa Toda, Megumi Nakamura, Hiraku Morisawa, Machiko Iwamoto, Junpei Ohkata, Hajime Sato, Hiroshi Tanaka, Kazutomi Kanemaru
  • Organizer: MPSA2008 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 20080826-20080829