2010 Fiscal Year Final Research Report
Neurobehavioral changes in metallothionein-null mice postnatal exposed to low levels mercury vapor.
| Project/Area Number |
20590611
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Hachinohe University |
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Principal Investigator |
YOSHIDA Minoru Hachinohe University, 人間健康学部, 教授 (80081660)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Masahiko 愛知学院大学, 薬学部, 教授 (20256390)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Chiho 東京大学, 大学院・医学研究科, 教授 (70220902)
YASUTAKE Akira 国立水俣病総合研究センター, 生化学室長 (20344418)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 神経行動毒性 / 水銀蒸気曝露 / 新生児 / メタロチオネイン / マイクロアレイ |
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| Research Abstract |
Metallothionein MT-null and wild-type mice were repeatedly exposed to Hg^0 at 0.057 mg/m^3, which were similar to the current threshold value (TLV), until the 20th of postpartum. Hg^0-exposed mice of both strains did not show behavioral changes such as total locomotor activity, learning ability and spatial learning ability. However, Hg^0 exposure resulted in significant alterations in gene expression in the brain of both strains using DNA microarray analysis. A number of altered genes in MT-null mice were more than those in wild-type mice. When the exposure levels elevated to 0.198mg/m^3, MT-null mice showed a significant decrease in total locomotor activity in female, though learning ability and spatial learning ability were not affected. A number of altered genes were further increased by elevation of Hg^0 exposure levels. DNA microarray analysis may provide useful information to elucidate development of behavioral toxicity following a low-level exposure to Hg^0.
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