2010 Fiscal Year Final Research Report
STR genotyping of the DNA fragments from fingerprint-lines captured by using a laser microdissection method
| Project/Area Number |
20590684
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Saitama Medical University |
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Principal Investigator |
HARA Masaaki Saitama Medical University, 医学部, 講師 (50129160)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Kazuyuki 埼玉医科大学, 医学部, 教授 (10215535)
TAKADA Aya 埼玉医科大学, 医学部, 講師 (30245196)
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| Project Period (FY) |
2008 – 2010
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| Keywords | レーザーマイクロダイゼクション / 指紋 / STR / リアルタイムPCR |
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| Research Abstract |
The short tandem repeat (STR) genotyping of the extracted DNA from the single print of tape adhere the fingerprint (200mm^2) were performed, resulted in STR genotyping of the single print could be identified easily. Next study, to reveal the DNA extracted from refined single line of fingerprint on the microslide glass, STR genotyping of DNA sample from the single line was performed. A STR genotyping was identifiable 12～6 luci in 10x6mm, 7～2 loci in 7x4mm and 4～1 loci in 7x2mm.
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Research Products
(13 results)
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[Journal Article] A novel method for efficient analysis of STR loci from a single sperm captured by laser microdissection.2008
Author(s)
T. Miyazaki, M.Hara, A. Ichiki, Y. Yamamoto, A.Takada, A.Kido, M. Nodera, H. Yanagisawa, H. Suzuki, K Saito
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Journal Title
Forensic Science International, Genetics Supplement Series 1
Pages: 437-438
Peer Reviewed
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