2010 Fiscal Year Final Research Report
Development of a novel cell source for regenerative medicine in elderly patients
| Project/Area Number |
20590707
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Nihon University |
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Principal Investigator |
MATSUMOTO Taro Nihon University, 医学部, 教授 (50366580)
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| Co-Investigator(Kenkyū-buntansha) |
KANO Koichiro 日本大学, 生物資源科学部, 准教授 (80271039)
IRIBE Yuji 日本大学, 医学部, 研究員 (20348618)
ISHIGE Mika 日本大学, 医学部, 助教 (90420950)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 老年医学 / 再生医療 / 脂肪細胞 / 脱分化脂肪細胞 / 血管新生 / 間葉系幹細胞 |
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| Research Abstract |
We examined efficient induction of osteogenic and angiogenic differentiation in mature adipocyte-derived dedifferentiated fat (DFAT) cells, which were prepared from adipose tissue of patients of various ages. The results showed that DFAT cells prepared form bone marrow adipocytes exhibited high osteogenic potential regardless of donor age. Conditioned media from cultured DFAT cells under low (1%) oxygen condition showed strong angiogenic activity. DFAT cell transplantation increased bone density in an animal model of osteoporosis induced by overiectomy. In a mouse ischemic hind limb model, an increase in blood flow was observed in DFAT cell-transplanted mice, compared to that in peripheral blood mononuclear cell-transplanted mice. These results suggest that DFAT cells may be an attractive cell source for cell-based therapy in elderly patients with osteoporosis and peripheral artery disease.
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