2010 Fiscal Year Final Research Report
Pulmonary collectin regulates drug-induced lung injury.
Project/Area Number |
20590930
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Respiratory organ internal medicine
|
Research Institution | Sapporo Medical University |
Principal Investigator |
TAKAHASHI Hiroki Sapporo Medical University, 医学部, 教授 (60231396)
|
Co-Investigator(Kenkyū-buntansha) |
KUROKI Yoshio 札幌医科大学, 医学部, 教授 (70161784)
|
Co-Investigator(Renkei-kenkyūsha) |
SHIRATORI Masanori 札幌医科大学, 医学部, 講師 (40295366)
CHIBA Hirofumi 札幌医科大学, 医学部, 助教 (40347175)
KUDO Kazumi 札幌医科大学, 医学部, 助教 (90438002)
KURONUMA Koji 札幌医科大学, 医学部, 助教 (40563250)
|
Project Period (FY) |
2008 – 2010
|
Keywords | 薬剤性肺障害 / ブレオマイシン / 肺コレクチン / 肺サーファクタント蛋白質A / トル様受容体 |
Research Abstract |
The purposes of this project were to investigate the mechanisms of regulation by which lung collectins, surfactant proteins A and D (SP-A and SP-D), and Toll-like receptor (TLR) function against inflammation on the development of drug induced lung injury, and to establish the molecular basis for the clinical application of these proteins. First, the mouse model of lung injury was established using drugs, and SP-A^<-/-> mice had significantly higher mortality rates than WT mice. SP-A inhibited drug-induced inflammatory cytokine production from rat alveolar macrophages. SP-A blocked sTLR2-bleomycin binding in the dose dependent manner. These results suggest that bleomycin signals are transmitted through TLR2 dependent pathway and regulated by the molecular interaction of SP-A.
|
Research Products
(11 results)
-
[Journal Article] Pulmonary Collectins Protect Macrophages against Pore-forming Activity of Legionella pneumophila and Suppress Its Intracellular Growth.2010
Author(s)
Sawada K, Ariki S, Kojima T, Saito A, Yamazoe M, Nishitani C, Shimizu T, Takahashi M, Mitsuzawa H, Yokota S, Sawada N, Fujii N, Takahashi H, Kuroki Y.
-
Journal Title
J Biol Chem 285
Pages: 8434-8443
Peer Reviewed
-
-
[Journal Article] Pulmonary surfactant protein D binds MD-2 through the carbohydrate recognition domain.2008
Author(s)
Nie X, Nishitani C, Yamazoe M, Ariki S, Takahashi M, Shimizu T, Mitsuzawa H, Sawada K, Smith K, Crouch E, Nagae H, Takahashi H, Kuroki Y.
-
Journal Title
Biochemistry 47
Pages: 12878-12885
Peer Reviewed
-
[Journal Article] Pulmonary Surfactant Protein D Inhibits Lipopolysaccharide (LPS)-induced Inflammatory cell responses by altering LPS binding to its receptors.2008
Author(s)
Yamazoe M, Nishitani C, Takahashi M, Katoh T, Ariki S, Shimizu T, Mitsuzawa H, Sawada K, Voelker DR, Takahashi H, Kuroki Y.
-
Journal Title
J Biol Chem 283
Pages: 35878-35888
Peer Reviewed
-
-
-
[Presentation] Pulmonary collectins exhibit bactericidal activity against Legionella pneumophila.2008
Author(s)
Sawada K, Ariki S, Yamazoe M, Nishitani C, Shimizu T, Takahashi M, Yokota S, Fujii N, Takahashi H, Kuroki Y.
Organizer
In : The 8^<th> Awaji International Forum on Infection and Immunity
Place of Presentation
Awaji, Japan
Year and Date
20080907-20080912
-
-
-
-
[Remarks] ホームページ等