2010 Fiscal Year Final Research Report
Exploration for novel murine hematopoietic stem cells by the use of intra-bone marrow injection methods
| Project/Area Number |
20591158
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
SASAKI Yutaka Kansai Medical University, 医学部, 講師 (80425066)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SONODA Yoshiaki 関西医科大学, 医学部, 教授 (60206688)
HASE Makoto 関西医科大学, 医学部, 助教 (50425203)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 造血幹細胞 / 骨髄内移植 / c-kit / 移植・再生医療 |
|---|
| Research Abstract |
Intra-bone marrow injection (IBMI) is a powerful method that enables highly efficient hematopoietic stem cell (HSC) engraftment in recipients' bone marrow. Using this method, novel HSCs that are not included in the c-kit^+Sca-1^+Lineage^-(KSL) compartment are explored. IBMI did not detect reproducibly the HSC activity in c-kit^-Sca^-1^+Linfraction, although in some rare cases, long-term hematopoietic reconstitution was observed in the fraction. Therefore, possibility remains that very rare HSCs exist in c-kit-fraction. On the other hand, c-kit^low fraction was proved to contain HSCs in low frequency. These HSCs were characterized.
|
Research Products
(6 results)
