2010 Fiscal Year Final Research Report

Analysis of mechanism for blister formation in pemphigus foliaceus using novel monoclonal antibody with pathogenic activity

Research Project

Project/Area Number	20591329
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Dermatology
Research Institution	Keio University
Principal Investigator	ISHIKO Akira Keio University, 医学部, 講師 (10202988)
Co-Investigator(Kenkyū-buntansha)	FUNAKOSHI Takeru 慶應義塾大学, 医学部, 助教 (80365353) ISHII Ken 慶應義塾大学, 医学部, 助教 (50296670) OUCHI Takeshi 慶應義塾大学, 医学部, 助教 (30528419) SHIMIZU Atsushi 慶應義塾大学, 医学部, 共同研究員 (60383733)
Project Period (FY)	2008 – 2010
Keywords	自己免疫 / 免疫電顕 / 水疱症 / デスモソーム / 天疱瘡
Research Abstract	Pemphigus foliaceus (PF) is an autoimmune disease characterized by blister and erosion formation over the whole body surface. The mechanism of blister formation after antibody binding to the antigen has not been fully elucidated. In this study, we observed ultrastructural change after injection of monoclonal antibody derived from the patient with PF, to the organ cultured normal human skin. As results, number of desmosomes was markedly decreased in 2 hours, and disappearance of desmosome and cell detachment were observed 22 hours afterward. This result suggested the presence of the pathway of cell detachment with the decrease of desmosome.

(1 results)