2010 Fiscal Year Final Research Report
Establishment of rhodopsin transgenic rabbit line and analysis of retinal degeneration
| Project/Area Number |
20592075
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Nagoya University |
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Principal Investigator |
KONDO Mineo Nagoya University, 大学院・医学系研究科, 准教授 (80303642)
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| Co-Investigator(Kenkyū-buntansha) |
TERASAKI Hiroko 名古屋大学, 大学院・医学系研究科, 教授 (40207478)
ITO Yasuki 名古屋大学, 大学院・医学系研究科, 特任准教授 (10313991)
KACHI Shu 名古屋大学, 医学部附属病院, 講師 (30345904)
KOMEIMA Keiichi 名古屋大学, 大学院・医学系研究科, 助教 (40362256)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 網膜色素変性 / 動物モデル / ウサギ / 網膜電図 / トランスジェニック |
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| Research Abstract |
We have generated a rabbit model of retinitis pigmentosa (RP), the rhodopsin P347L transgenic (Tg) rabbit, by using bacterial artificial chromosome (BAC) transgenesis. These rabbits showed a rod-dominant, progressive retinal degeneration with a marked regional variations in the loss of photoreceptors. All of the electroretinogram components of the Tg rabbits decrease progressively with the a-wave more affected than the b-wave, and the oscillatory potentials (OPs) were the best preserved. Interestingly, the OP amplitudes of young Tg rabbits were significantly larger than those of wild-type rabbits. Pharmacological experiments showed that the significantly larger OPs in young Tg rabbits resulted from secondary alterations in the inner retinal function.
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[Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade.2010
Author(s)
Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A, Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T.
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Journal Title
Proc Natl Acad Sci USA. 107
Pages: 332-337
Peer Reviewed
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[Journal Article] Pikachurin, a dystroglycan ligand, is essential for photoreceptor ribbon synapse formation.2008
Author(s)
Sato S, Omori Y, Katoh K, Kondo M, Kanagawa M, Miyata K, Funabiki K, Koyasu T, Kajimura N, Miyoshi T, Sawai H, Kobayashi K, Tani A, Toda T, Usukura J, Tano Y, Fujikado T, Furukawa T.
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Journal Title
Nat Neurosci. 11
Pages: 923-931
Peer Reviewed
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