2010 Fiscal Year Final Research Report
Gene therapy using sonoporation for maxillofacial distraction
| Project/Area Number |
20592340
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Kyushu Dental College |
|---|
Principal Investigator |
YOSHIOKA Izumi Kyushu Dental College, 医学部, 准教授 (10305823)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAKODA Sumio 宮崎大学, 医学部, 教授 (20118079)
IGAWA Kaori 宮崎大学, 医学部, 助教 (90423722)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 顎骨延長法 / BMP |
|---|
| Research Abstract |
Bone morphogenetic protein-2 (BMP-2) is expected to be utilized to fill bone defects and promote healing of fractures. However, it is unable to generate an adequate clinical response for use in bone regeneration. Recently, it was reported that glycosaminoglycans, including heparin, heparan sulfate, keratan sulfate, dermatan sulfate, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid (HA), regulate BMP-2 activity, though the mechanism by which HA regulates osteogenic activities has not been fully elucidated. The aim of this study was to investigate the effects of HA on osteoblast differentiation induced by BMP-2.Monolayer cultures of osteoblastic lineage MG63 cells were incubated with BMP-2 and HA for various time periods. To determine osteoblastic differentiation, alkaline phosphatase (ALP) activity in the cell lysates was quantified. Phosphorylation of Smad 1/5/8, p38, and ERK proteins was determined by Western blot analysis. To elucidate the nuclear translocation of phosphorylated Smad 1/5/8, stimulated cells were subjected to immunofluorescence microscopy. To further elucidate the role of HA in enhancement of BMP-2-induced Smad signaling, mRNA expressions of the BMP-2 receptor antagonists noggin and follistatin were detected using real-time RT-PCR.BMP-2-induced ALP activation, Smad 1/5/8 phosphorylation, and nuclear translocation were up-regulated when MG63 cells were cultured with both BMP-2 and HA. Western blot analysis revealed that phosphorylation of ERK protein was diminished by HA. Furthermore, the mRNA expressions of noggin and follistatin induced by BMP-2 were preferentially blocked by HA.These results indicate that HA enhanced BMP-2 induces osteoblastic differentiation in MG63 cells via down-regulation of BMP-2 antagonists and ERK phosphorylation.
|
-
-
[Journal Article] Relationship Between Inferior Alveolar Nerve Canal Position at Mandibular Second Molar in Patients With Prognathism and Possible Occurrence of Neurosensory2010
Author(s)
Yoshioka I, Tanaka T, Khanal A, Habu M, Kito S, Kodama M, Oda M, Wakasugi-Sato N, Matsumoto-Takeda S, Fukai Y, Tokitsu T, Tomikawa M, Seta Y, Tominaga K, Morimoto Y
-
Journal Title
Disturbance After Sagittal Split Ramus Osteotomy J Oral Maxillofac Surg. 68
Pages: 3022-3027
-
-
-
-
-
-
-
-
[Remarks] ホームページ等
