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2010 Fiscal Year Final Research Report

The study on the role of extrinsic proteins in assembly of photosystem 2 complex

Research Project

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Project/Area Number 20608002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 光生命科学
Research InstitutionThe University of Tokyo

Principal Investigator

MIZUSAWA Naoki  The University of Tokyo, 大学院・総合文化研究科, 助教 (80342856)

Project Period (FY) 2008 – 2010
Keywords植物生理 / シアノバクテリア / 光合成 / 光化学系II / 表在性蛋白質
Research Abstract

Mechanism of assembly of photosystem II complex (PSII) has been investigated focusing on the role of extrinsic proteins in the assembly process of PSII. Psb28 is a newly identified extrinsic protein bound to PSII but its function was unknown. In this study, we analyzed properties of PSII in a disruption mutant of psb28 gene of cyanobacterium. It was found that, in the psb28 mutant, PSII assembly intermediates were accumulated and that a repair process of PSII was significantly inhibited. These results suggest that Psb28 plays an important role in the repair process of PSII.

  • Research Products

    (20 results)

All 2011 2010 2009 2008

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (14 results) Book (1 results)

  • [Journal Article] Synthesis of fatty acids de novo is required for photosynthetic acclimation of Synechocystis sp.PCC 6803 to high temperature.2010

    • Author(s)
      Nanjo Y., Mizusawa N., Wada H., Slabas A.R., Hayashi H., Nishiyama Y.
    • Journal Title

      Biochim.Biophys.Acta 1797

      Pages: 1483-1490

    • Peer Reviewed
  • [Journal Article] Purification and characterization of photosystem I complex from Synechocystis sp.PCC 6803 by expressing histidine-tagged subunits.2010

    • Author(s)
      Kubota H., Sakurai I., Katayama K., Mizusawa N., Ohashi S., Kobayashi M., Zhang P., Aro E.M., Wada H.
    • Journal Title

      Biochim.Biophys.Acta 1797

      Pages: 98-105

    • Peer Reviewed
  • [Journal Article] Involvement of digalactosyldiacylglycerol in cellular thermotolerance of Synechocystis sp.PCC 6803.2009

    • Author(s)
      Mizusawa N., Sakata S., Sakurai I., Sato N., Wada H.
    • Journal Title

      Arch.Microbiol. 191

      Pages: 595-601

    • Peer Reviewed
  • [Journal Article] Lack of digalactosyldiacylglycerol increases the sensitivity of Synechocystis sp.PCC 6803 to high light stress.2009

    • Author(s)
      Mizusawa N., Sakurai I., Sato N., Wada H.
    • Journal Title

      FEBS Lett. 783

      Pages: 718-722

    • Peer Reviewed
  • [Journal Article] Role of phosphatidylglycerol in oxygen-evolving complex of photosystem II.2008

    • Author(s)
      Mizusawa N., Sakurai I., Kubota H., Wada H.
    • Journal Title

      In Energy from the Sun(Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B., eds.)(Springer.)

      Pages: 463-466

    • Peer Reviewed
  • [Presentation] 高温ストレス下でのSynechocystis sp.PCC 6803におけるPsb28の機能に関する研究2011

    • Author(s)
      水澤直樹、酒田慎也、久保田寿子、桜井勇、和田元
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      東北大学川内キャンパス、仙台
    • Year and Date
      20110320-20110322
  • [Presentation] Essential role of digalactosyldiacylglycerol for photosynthetic growth in Synechocystis sp.PCC 6803 under high-light and high-temperature stresses.2011

    • Author(s)
      Mizusawa N., Sakata S., Sakurai I., Kubota H., Sato N., Wada H.
    • Organizer
      日本-フィンランド交換セミナー2010
    • Place of Presentation
      岡山ロイヤルホテル、岡山
    • Year and Date
      20110302-20110305
  • [Presentation] Roles of anionic phospholipids, phosphatidylglycerol and cardiolipin, in photosynthetic organisms.2011

    • Author(s)
      Wada H., Katayama K., Tanoue R., Mizusawa N.
    • Organizer
      日本-フィンランド交換セミナー2010
    • Place of Presentation
      岡山ロイヤルホテル、岡山
    • Year and Date
      2011-03-03
  • [Presentation] Essential role of digalactosyldiacylglycerol for photosynthetic growth in Synechocystis sp.PCC 6803 under high-temperature stress.2010

    • Author(s)
      Mizusawa N., Sakata S., Sakurai I., Kubota H., Sato N., Wada H.
    • Organizer
      15th International congress on photosynthesis.
    • Place of Presentation
      BeiJing friendship hotel, 北京
    • Year and Date
      20100822-20100827
  • [Presentation] Synechocystis sp.PCC 6803の光化学系IにおけるDGDGの機能2010

    • Author(s)
      久保田寿子、水澤直樹、和田元
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      熊本大学黒髪北キャンパス、熊本
    • Year and Date
      20100318-20100321
  • [Presentation] 高温ストレス下でのシアノバクテリアの光合成におけるジガラクトシルジアシルグリセロールの役割2009

    • Author(s)
      水澤直樹、酒田慎也、桜井勇、久保田寿子、佐藤直樹、和田元
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2009」
    • Place of Presentation
      かずさアカデミアホール、木更津
    • Year and Date
      20091204-20091205
  • [Presentation] シアノバクテリアにおける光化学系II表在性タンパク質の脂質修飾に関する研究2009

    • Author(s)
      久保田寿子、栗原梓、水澤直樹、和田元
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Year and Date
      20090321-20090324
  • [Presentation] ジガラクトシルジアシルグリセロールの欠乏はSynechocystis sp.PCC 6803の熱感受性を増大させる2009

    • Author(s)
      水澤直樹、酒田慎也、桜井勇、佐藤直樹、和田元
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Year and Date
      20090321-20090324
  • [Presentation] 光化学系II複合体アセンブリーに関与するPsb27タンパク質の機能解析2009

    • Author(s)
      内海真希、久保田寿子、水澤直樹、和田元
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Year and Date
      20090321-20090324
  • [Presentation] シアノバクテリアにおけるPsb28タンパク質の機能解析2009

    • Author(s)
      酒田慎也、久保田寿子、桜井勇、水澤直樹、和田元
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Year and Date
      20090321-20090324
  • [Presentation] 光化学系I複合体の簡易精製と新規成分の同定2009

    • Author(s)
      和田元、久保田寿子、片山健太、桜井勇、水澤直樹
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2009」
    • Place of Presentation
      かずさアカデミアホール、木更津
    • Year and Date
      2009-12-05
  • [Presentation] 光化学系IIの高温適応における脂肪酸合成の役割2009

    • Author(s)
      西山佳孝、南條洋平、水澤直樹、和田元、林秀則
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2009」
    • Place of Presentation
      かずさアカデミアホール、木更津
    • Year and Date
      2009-12-05
  • [Presentation] 光合成を支える脂質の機能2009

    • Author(s)
      水澤直樹、桜井勇、酒田慎也、久保田寿子、佐藤直樹、和田元
    • Organizer
      ラン藻ゲノム研究交流会
    • Place of Presentation
      東京大学教養学部、東京
    • Year and Date
      2009-07-04
  • [Presentation] Simple purification and characterization of PSI complex from the Synechocystis sp.PCC 6803 strains expressing His-tagged subunit.2008

    • Author(s)
      Kubota H., Sakurai I., Mizusawa N., Zhang P., Aro E.M., Wada H.
    • Organizer
      7th European workshop on molecular biology of cyanobacteria
    • Place of Presentation
      University of South Bohemia, Ceske Budejovice, Czech Republic
    • Year and Date
      20080831-20080904
  • [Book] Lipids in Photosynthesis : Essential and Regulatory Function(Wada, H., Murata, N. eds.)(The role of phosphatidylglycerol in photosynthesis.)2009

    • Author(s)
      Wada H., Mizusawa N.
    • Total Pages
      243-263
    • Publisher
      Springer

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Published: 2012-01-26   Modified: 2016-04-21  

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