2010 Fiscal Year Final Research Report
The next generation structural biology using a new pulsed-neutron diffractometer
| Project/Area Number |
20612002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Quantum beam science
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| Research Institution | Ibaraki University |
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Principal Investigator |
TANAKA Ichiro Ibaraki University, 工学部, 准教授 (20354889)
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| Co-Investigator(Kenkyū-buntansha) |
TOMOYORI Katsuaki 茨城大学, フロンティア応用原子科学研究センター, 産学官連携研究員 (10455909)
YAMADA Taro 茨城大学, フロンティア応用原子科学研究センター, 助教 (40455910)
KUSAKA Katsuhiro 茨城大学, フロンティア応用原子科学研究センター, 准教授 (10414591)
OHNISHI Yuuki 茨城大学, 工学部, 産学官連携研究員 (50455911)
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| Co-Investigator(Renkei-kenkyūsha) |
NIIMURA Nobuo 茨城大学, フロンティア応用原子科学研究センター, 特任教授 (50004453)
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| Project Period (FY) |
2008 – 2010
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| Keywords | J-PARC / 生体高分子用中性子回折計(iBIX) / 中性子結晶解析 / 水素 / 水素結合 / タンパク質 / 水和水 |
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| Research Abstract |
A new diffractometer for protein crystallography (iBIX) at the next generation pulsed-neutron source at J-PARC, has been completed and operational since the end of 2008. After various commissionings, data collection of ribonuclease A could be done at the accelerator power of 120kW for a crystal of 5 mm3 in volume for about 10 days as the first protein sample at iBIX. As a result of structural analysis, hydrogen position and orientation of waters for a purpose could be confirmed. According to the result, it turned out that it is possible to collect a full data set of a protein in about 3 days for a crystal of 1 mm3 in volume when J-PARC accelerator power becomes 1MW and 30 detectors of iBIX are provided as a total as scheduled.
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