2011 Fiscal Year Final Research Report
Evaluation of the Embryo Quality for Elective Single Embryo Transfer
| Project/Area Number |
20680026
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2011
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| Keywords | バイオメカニクス / ヒト生殖補助医療 |
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| Research Abstract |
To enable improved success rate of IVF, we developed the technology to optimize embryo selection, having the highest implantation potential while ensuring no damage to embryos using zona elasticity as selection criteria. First, "zona hardening" was demonstrated mechanically following fertilization at the PN stage, Young's modulus decreased gradually as the embryo developed. Evaluation of the quality of expanded blastocysts(EPB) shows that the quality of EPBs could also be evaluated from elasticity parameters. Furthermore observations indicated that ZPs of embryos generated in vivo were significantly harder than those of embryos generated in vitro at each stage. We examined the correlations between subsequent ZP elasticity changes after IVF treatment and quality of embryos and the results showed that the pregnancy rate after MTS-eSET was almost the same with that after double-embryo transfer. It was also examined that both theoretically and experimentally the MTS test does not cause any damage to embryos. Furthermore, we developed a cryo-nano-hole vitrification container with a low concentration of CPA solution with no serum and mouse and human blastocysts can be cryopreserved successfully.
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Research Products
(10 results)
