2011 Fiscal Year Final Research Report
Pressure controlling of DNA structure and transcription
| Project/Area Number |
20680027
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
KIMURA Tsuyoshi 東京医科歯科大学, 生体材料工学研究所, 助教 (10393216)
|
|---|
| Project Period (FY) |
2008 – 2011
|
| Keywords | 静水圧 / 高次構造 / 凝縮 / 転写 / 遺伝子送達 |
|---|
| Research Abstract |
In this study, we have developed a novel compaction method of DNA using high hydrostatic pressure technology for gene delivery. It was founded that the compaction and secondary structure of DNA was controlled by the regulation of the high hydrostatic pressuring condition. When the compacted DNA by high hydrostatic pressure was applied for in vitro and in vivo transfection, the gene transcription and expression were delayed, suggesting that the controlled compaction of DNA by high hydrostatic pressurization would be regulate the transgene expression.
|
