2010 Fiscal Year Final Research Report
Oncogenic role of APC/C ubiquitin ligase inhibitor, Emi1
| Project/Area Number |
20689033
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
KUDO Yasusei Hiroshima University, 大学院・医歯薬学総合研究科, 助教 (50314753)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 癌 / 細胞周期 / ユビキチン分解 / 細胞分裂 |
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| Research Abstract |
The abnormality of checkpoint and cell division during cell cycle is thought to be a trigger of carcinogenesis. APC/C ubiquitin ligase complex regulates the protein level of many key molecules for cell division. Emi1, an inhibitor of APC/C, inhibits APC/C activity from S phase to the beginning of M phase. In the present study, we found that APC/C inhibitor, Emi1 was frequently overexpressed in cancer cell lines and head and neck cancer cases. Moreover, we found a cancer cell line that constitutively expressed Emi1 during cell cycle progression. In this cell line, Ser310 and Thr315 of Emi1 were phosphorylated by ERK-RSK pathway and this phosphorylation inhibited ubiquitin-mediated proteolysis. Constitutive Emi1 expression inhibited APC/C activity during cell cycle progression and induced overexpression of APC/C substrates.In summary, Emi1 overexpression caused by the defects of degradation induced the constitutive inhibition of APC/C activity and was involved in abnormal regulation of cell cycle and carcinogenesis.
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Research Products
(32 results)
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[Journal Article] miR-22 provides a direct link between celular senescence and tumorgenesis.2011
Author(s)
Xu, D., Takeshita, F., Hino, Y., Fukunaga, S., Kudo, Y., Tamaki, A., Matsunaga, J., Takahashi, R., Takata, T., Shimamoto, A., Ochiya, T., Tahara, H.
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Journal Title
J Cell Biol 93
Pages: 409-424
Peer Reviewed
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[Journal Article] Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via crosstalk between integrin β1 and CD63.2011
Author(s)
Iizuka, S., Kudo, Y., Yoshida, M., Tsunematsu, T., Yoshiko, Y., Uchida, T., Ogawa, I., Miyauchi, M., Takata, T.
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Journal Title
Mol Cell Biol Corresponding author 31
Pages: 783-792
Peer Reviewed
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[Journal Article] N-cadherin expression is correlated with metastasis of spindle cell carcinoma of head and neck region.2011
Author(s)
Nguyen, P.T., Kudo, Y., Yoshida, M., Iizuka, S., Ogawa, I., Takata, T.
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Journal Title
J Oral Pathol Med Corresponding author 40
Pages: 77-82
Peer Reviewed
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[Journal Article] N-cadherin expression is involved in malignant behavior of head and neck cancer in relation to epithelial-mesenchymal transition.2011
Author(s)
Nguyen, P.T., Kudo, Y., Yoshida, M., Kamata, N., Ogawa, I., Takata, T.
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Journal Title
Histology and Histopathology Corresponding author 26
Pages: 147-156
Peer Reviewed
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[Journal Article] Nuclear Survivin expression is correlated with malignant behaviors of head and neck cancer together with Aurora-B.2010
Author(s)
Qi G, Kudo, Y., Ando T, Tsunematsu T, Shimizu N, Siriwardena S, Yoshida M, Keikhaee M, Ogawa I, Takata T.
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Journal Title
Oral Oncol Corresponding author 46
Pages: 263-270
Peer Reviewed
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[Journal Article] Oncogenic role of nuclear accumulated Aurora-A.2009
Author(s)
Tatsuka, M., Sato, S., Kanda, A., Miki, T., Kamata, N., Kitajima, S., Kudo, Y., Takata, T.
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Journal Title
Mol Carcinogen 48
Pages: 810-820
Peer Reviewed
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[Journal Article] RUNX3 has an oncogenic role in head and neck cancer.2009
Author(s)
Tsunematsu, T., Kudo, Y., Iizuka, S., Ogawa, I., Fujita, T., Kurihara, H., Abiko, Y., Takata, T.
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Journal Title
PLoS ONE Corresponding author 4
Pages: e5892
Peer Reviewed
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[Journal Article] SCF^BTrcp mediates stress-induced Cdc25B ubiquitylation through cooperation of an atypical consensus sequence and PEST.
Author(s)
Uchida, S., Watanabe, N., Kudo, Y., Yoshioka, K., Matsunaga, T., Ishizuka, Y., Nakagama, H., Poon, R.Y.C., Yamashita, K.
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Journal Title
Peer Reviewed
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