2009 Fiscal Year Final Research Report
Quantitative analysis of reciprocal connection between the thalamus and neocortex by using lentiviral vectors
| Project/Area Number |
20700315
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto University |
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Principal Investigator |
HIOKI Hiroyuki Kyoto University, 大学院・医学研究科, 助教 (00402850)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 神経回路網 |
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| Research Abstract |
We developed novel lentiviral vectors by using "Tet-Off system" and succeeded in achieving high-level and neuron-specific gene transduction in vivo. One week after viral injection into the rat neostriatum, the GFP expression was almost completely neuron-specific and about 40 times higher than the expression of a conventional lentiviral vector. High transcriptional activity and neuronal specificity were sustained for up to 8 weeks. Furthermore, neuronal processes of the infected neurons were efficiently visualized by adding a plasma membrane-targeting signal to GFP. These results suggest that the present method is valuable for strong gene transduction and clear visualization of neurons in vivo.
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[Journal Article] Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells.2010
Author(s)
Ohira K, Furuta T, Hioki H, Nakamura KC, Kuramoto E, Tanaka Yasuyo, Funatsu N, Shimizu K, Oishi T, Hayashi M, Miyakawa T, Kaneko T, Nakamura S
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Journal Title
Nature Neuroscience 13(2)
Pages: 173-179
Peer Reviewed
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[Journal Article] Inhibitory and ex citatory subtypes of cochlear nucleus neurons are defined by distinct bHLH transcription factors, Ptf1a and Atoh1.2009
Author(s)
Fujiyama T, Yamada M, Terao M, Terashima T, Hioki H, Inoue YU, Inoue T, Masuyama N, Obata K, Yanagawa Y, Kawaguchi Y, Nabeshima Y, *Hoshino M
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Journal Title
Development 136(12)
Pages: 2049-2058
Peer Reviewed
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[Presentation] 成熟期大脳皮質に存在する神経前駆細胞2009
Author(s)
大平耕司, 古田貴寛, 日置寛之, 中村公一, 倉本恵梨子, 船津宣雄, 清水慶子, 大石高生, 林基治, 宮川剛, 金子武嗣, 中村俊
Organizer
第32回日本神経科学学会
Place of Presentation
名古屋
Year and Date
2009-09-17
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[Presentation] 大脳皮質興奮性細胞から皮質視床投射細胞への皮質内入力の定量2009
Author(s)
田中康裕, 田中康代, 今野美知輝, 藤山文乃, 岡本ー古田敬子, 薗村貴弘, 亀田浩司, 日置寛之, 古田貴寛, 中村公一, 金子武嗣
Organizer
第32回日本神経科学学会
Place of Presentation
名古屋
Year and Date
2009-09-17
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